9ECJ: Human P2RY8

Human P2RY8 bound to S-geranylgeranyl-L-glutathione in complex with miniG13. Determined by electron microscopy at 2.9 Å resolution. Released 5 Nov 2025.

Method: Electron microscopy
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 3
Atoms: 6,323
Mol. weight: 112.34 kDa
Ligands: A1BIG
Released: 5 Nov 2025

Explore 9ECJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9ECJ contains 25 α-helices and 37 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 16 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	11-16	6
α-helix	22-45	24
α-helix	46-50	5
α-helix	57-84	28
α-helix	93-126	34
α-helix	130-133	4
α-helix	137-154	18
α-helix	157-160	4
β-strand	164-167	4	1
β-strand	172-175	4	1
α-helix	187-198	12
α-helix	199-203	5
α-helix	204-222	19
α-helix	229-267	39
α-helix	272-283	12
α-helix	285-289	5
α-helix	290-295	6
α-helix	298-301	4

Chain C: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	42-45	4
β-strand	49-53	5	2
α-helix	61-71	11
β-strand	207-213	7	2
β-strand	216-222	7	2
α-helix	234-237	4
β-strand	242-248	7	2
α-helix	252-266	25
α-helix	269-272	4
β-strand	275-281	7	2
α-helix	283-289	7
α-helix	296-298	3
α-helix	309-321	13
β-strand	332-336	5	2
α-helix	344-363	20

Chain D: 0 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	43	1	3
β-strand	47-52	6	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	91-94	4	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	120-125	6	6
β-strand	134-140	7	6
β-strand	146-151	6	7
β-strand	156-161	6	7
β-strand	166-170	5	7
β-strand	175-180	6	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	209-212	4	8
β-strand	218-221	4	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
β-strand	273-278	6	3
β-strand	284-289	6	3
β-strand	294-298	5	3
β-strand	306-308	3	3
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	335-339	5	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
P2Y purinoceptor 8	B	protein	384	Homo sapiens	Q86VZ1 (AlphaFold model)
Guanine nucleotide-binding protein subunit alpha-13	C	protein	240	Homo sapiens	Q14344 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	D	protein	370	Homo sapiens	P62873 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>9ECJ_1 P2Y purinoceptor 8 (chains B)
FADYKDDDDMQVPNSTGPDNATLQMLRNPAIAVALPVVYSLVAAVSIPGNLFSLWVLCRR
MGPRSPSVIFMINLSVTDLMLASVLPFQIYYHCNRHHWVFGVLLCNVVTVAFYANMYSSI
LTMTCISVERFLGVLYPLSSKRWRRRRYAVAACAGTWLLLLTALSPLARTDLTYPVHALG
IITCFDVLKWTMLPSVAMWAVFLFTIFILLFLIPFVITVACYTATILKLLRTEEAHGREQ
RRRAVGLAAVVLLAFVTCFAPNNFVLLAHIVSRLFYGKSYYHVYKLTLCLSCLNNCLDPF
VYYFASREFQLRLREYLGCRRVPRDTLDTRRESLFSARTTSVRSEAGAHPEGMEGATRPG
LQRQESVFGSSGLEVLFQGPGGSG

Sequence of entity 2 (C), FASTA

>9ECJ_2 Guanine nucleotide-binding protein subunit alpha-13 (chains C)
MGSTVSAEDKAAAERSKEIDKCLSREKTYVKRLVKILLLGADNSGKSTFLKQMRIIHGGS
GGSGGTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVDSSDFDQVLM
EDRLTNRLTESLNDFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPH
CLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENARLIFRDVKDTILHDNLKQLMLQ

Sequence of entity 3 (D), FASTA

>9ECJ_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
MHHHHHHLEVLFQGPEDQVDPRLIDGKGSSGSELDQLRQEAEQLKNQIRDARKACADATL
SQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKV
HAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCR
FLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKL
WDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNII
CGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATG
SWDSFLKIWN

Ligands and cofactors

ID	Name	Formula	Copies
A1BIG	L-gamma-glutamyl-S-[(2Z,6E)-3,7,15-trimethyl-11-methylidenehexadeca-2,6,14-trie…	C30 H49 N3 O6 S	1

Primary citation

Human P2RY8 bound to S-geranylgeranyl-L-glutathione in complex with miniG13. LaFlam, T.N., Billesboelle, C.B., Dihn, T. et al. To be published.

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