9EHD: N-SH2 domain of SHP2

Crystal structure of N-SH2 domain of SHP2 bound to GAB1 tyrosine phosphorylated peptide (624-633) QVEpYLDLDLD. Determined by X-ray diffraction at 1.59 Å resolution. Released 7 Jan 2026.

Method: X-ray diffraction
Resolution: 1.59 Å
Organism: Homo sapiens
Chains: 2
Atoms: 945
Mol. weight: 13.51 kDa
Released: 7 Jan 2026

Explore 9EHD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9EHD contains 2 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	7	1	1
α-helix	13-23	11
β-strand	28-33	6	1
β-strand	41-47	7	1
β-strand	50-56	7	1
β-strand	57-58	2	2
β-strand	63-64	2	2
β-strand	71	1	2
α-helix	74-83	10
β-strand	89	1	3
β-strand	90	1	4
β-strand	95	1	3
β-strand	100-101	2	1

Chain B: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	8	1	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 1 of Tyrosine-protein phosphatase non-receptor type 11	A	protein	109	Homo sapiens	Q06124 (AlphaFold model)
Phosphorylated peptide from GRB2-associated-binding protein 1	B	protein	10	Homo sapiens	Q13480 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9EHD_1 Isoform 1 of Tyrosine-protein phosphatase non-receptor type 11 (chains A)
GSGMTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQ
NTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCAD

Sequence of entity 2 (B), FASTA

>9EHD_2 Phosphorylated peptide from GRB2-associated-binding protein 1 (chains B)
QVEYLDLDLD

Primary citation

Phosphatase SHP2 pathogenic mutations enhance activity by altering conformational sampling. Glaser, A.W., Padua, R.A.P., Ojoawo, A.M. et al. Proc Natl Acad Sci U S A (2026) 123:e2513851123-e2513851123. DOI 10.1073/pnas.2513851123 · PubMed

Other PDB entries of the same protein (UniProt Q06124 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9EIK 1.25 Å, Crystal structure of N-SH2 domain of SHP2 with T42A mutation bound to GAB1 tyrosine…
3ZM1 1.4 Å, Catalytic domain of human SHP2
4JMG 1.4 Å, Crystal structure of the synthetic protein in complex with pY peptide
7PPM 1.48 Å, SHP2 catalytic domain in complex with IRS1 (889-901) phosphopeptide (pSer-892, pTyr-896)
3ZM0 1.5 Å, Catalytic domain of human SHP2
3ZM2 1.5 Å, Catalytic domain of human SHP2
3ZM3 1.5 Å, Catalytic domain of human SHP2
7PPL 1.53 Å, SHP2 catalytic domain in complex with IRS1 (625-639) phosphopeptide (pTyr-632, pSer-636)
9EIC 1.58 Å, Crystal structure of unbound N-SH2 domain of SHP2 with T42A mutation
3B7O 1.6 Å, Crystal structure of the human tyrosine phosphatase SHP2 (PTPN11) with an accessible…
4RDD 1.6 Å, Co-crystal structure of SHP2 in complex with a Cefsulodin derivative
5EHR 1.7 Å, Non-receptor Protein Tyrosine Phosphatase SHP2 in Complex with Allosteric Inhibitor SHP099

Browse structure collections

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