9GC3: Yeast TFIIIC TauB subcomplex

yeast TFIIIC TauB subcomplex bound to a tRNA gene. Determined by electron microscopy at 2.46 Å resolution. Released 27 Nov 2024.

Method: Electron microscopy
Resolution: 2.46 Å
Organism: Saccharomyces cerevisiae
Chains: 5
Atoms: 15,046
Mol. weight: 305.47 kDa
Released: 27 Nov 2024

Explore 9GC3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9GC3 contains 78 α-helices and 109 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 35 helices, 23 β-strands

Element	Residues	Length	Sheet
α-helix	7-18	12
β-strand	24-26	3	1
α-helix	27-34	8
α-helix	43-54	12
β-strand	59-63	5	1
β-strand	66-67	2	1
α-helix	72-77	6
β-strand	83-87	5	1
α-helix	89-97	9
α-helix	108-120	13
α-helix	121-123	3
β-strand	125-126	2	2
α-helix	127-134	8
α-helix	138-146	9
α-helix	149-151	3
β-strand	152-159	8	2
β-strand	162-169	8	2
α-helix	170-172	3
α-helix	177-178	2
α-helix	185-187	3
α-helix	189-198	10
β-strand	203-205	3	3
α-helix	206-212	7
α-helix	215-217	3
α-helix	219-234	16
β-strand	238-244	7	3
β-strand	252-258	7	3
α-helix	334-344	11
α-helix	346-348	3
β-strand	350-351	2	4
α-helix	352-359	8
α-helix	362-364	3
α-helix	365-372	8
β-strand	376-378	3	4
β-strand	391-398	8	4
β-strand	401-408	8	4
α-helix	409-416	8
α-helix	421-426	6
α-helix	428-432	5
α-helix	438-444	7
α-helix	448-450	3
β-strand	453-457	5	5
β-strand	463-467	5	5
α-helix	473-475	3
β-strand	540	1	6
α-helix	542-544	3
β-strand	547	1	6
α-helix	550-565	16
β-strand	569-571	3	7
α-helix	574-583	10
α-helix	589-591	3
α-helix	592-604	13
β-strand	609-612	4	7
β-strand	619-622	4	7
α-helix	628-638	11
β-strand	648-650	3	3
β-strand	656	1	3
α-helix	660-667	8

Chain B: 17 helices, 41 β-strands

Element	Residues	Length	Sheet
β-strand	138-140	3	8
β-strand	144	1	9
α-helix	149-153	5
α-helix	160-162	3
α-helix	166-174	9
α-helix	178-191	14
α-helix	200-203	4
α-helix	216-218	3
α-helix	223-226	4
β-strand	234-238	5	10
α-helix	240-246	7
α-helix	253	1
β-strand	254-258	5	11
β-strand	261-265	5	11
β-strand	270-272	3	12
β-strand	282-287	6	12
β-strand	291-298	8	10
β-strand	303	1	13
β-strand	306	1	13
β-strand	310-318	9	10
α-helix	327-329	3
β-strand	339-347	9	10
β-strand	353-361	9	10
β-strand	366-371	6	14
β-strand	380-388	9	14
β-strand	393-399	7	14
α-helix	400-401	2
β-strand	407-413	7	10
β-strand	416-418	3	14
β-strand	420	1	15
β-strand	423	1	15
β-strand	425-430	6	16
β-strand	435-440	6	16
β-strand	444-449	6	16
β-strand	458-461	4	16
β-strand	467-473	7	17
β-strand	481-486	6	17
β-strand	490-494	5	17
α-helix	499-502	4
β-strand	504-508	5	17
β-strand	517-520	4	18
α-helix	521-523	3
β-strand	525-529	5	18
β-strand	534-539	6	18
β-strand	547-551	5	18
β-strand	556-560	5	11
β-strand	567-572	6	11
β-strand	576-581	6	11
α-helix	582-584	3
α-helix	593-596	4
β-strand	598-608	11	11
β-strand	613-617	5	11
β-strand	621-624	4	11
α-helix	625-626	2
α-helix	629-631	3
β-strand	642-647	6	12
β-strand	656-661	6	12
β-strand	665-670	6	12

Chain C: 26 helices, 45 β-strands

Element	Residues	Length	Sheet
α-helix	2-4	3
β-strand	5	1	19
α-helix	6	1
β-strand	7-10	4	20
β-strand	13	1	21
β-strand	21-23	3	22
β-strand	29-31	3	22
β-strand	34	1	21
β-strand	37-42	6	22
β-strand	51	1	19
α-helix	52-54	3
β-strand	56-61	6	22
β-strand	65-67	3	8
β-strand	71	1	9
β-strand	88-93	6	23
β-strand	100-104	5	23
β-strand	109-113	5	23
β-strand	116-120	5	23
α-helix	127-130	4
β-strand	132-137	6	24
β-strand	143-148	6	24
β-strand	152-160	9	24
β-strand	166-175	10	24
α-helix	178	1
α-helix	183-184	2
β-strand	185-191	7	25
β-strand	194-199	6	25
β-strand	204-208	5	25
α-helix	216-217	2
β-strand	218-222	5	25
β-strand	231-235	5	26
β-strand	238-243	6	26
β-strand	246-251	6	26
β-strand	257-261	5	26
α-helix	267-268	2
β-strand	269-272	4	27
β-strand	280-284	5	27
β-strand	289-293	5	27
β-strand	299-302	4	27
α-helix	307-321	15
α-helix	323-325	3
β-strand	329-338	10	20
β-strand	344-353	10	20
α-helix	361-363	3
β-strand	365-372	8	20
α-helix	386-393	8
α-helix	398-401	4
α-helix	410-411	2
α-helix	412-415	4
α-helix	423-431	9
α-helix	434-445	12
α-helix	452-463	12
α-helix	466-468	3
α-helix	472-485	14
β-strand	495-499	5	28
β-strand	504-508	5	28
α-helix	509-513	5
β-strand	518-520	3	29
β-strand	526-528	3	29
β-strand	529	1	30
α-helix	530	1
β-strand	536	1	30
β-strand	542-544	3	31
β-strand	551-553	3	31
α-helix	555-557	3
α-helix	565-573	9
β-strand	577	1	32
α-helix	583	1
β-strand	584	1	32
α-helix	585	1
β-strand	586-587	2	31

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Transcription factor tau 138 kDa subunit	A	protein	1201	Saccharomyces cerevisiae	P34111 (AlphaFold model)
Transcription factor tau 91 kDa subunit	B	protein	685	Saccharomyces cerevisiae	Q06339 (AlphaFold model)
Transcription factor tau 60 kDa subunit	C	protein	588	Saccharomyces cerevisiae	Q12308 (AlphaFold model)
DNA (40-mer)	D	DNA	40	Saccharomyces cerevisiae
DNA (40-mer)	E	DNA	40	Saccharomyces cerevisiae

Sequence of entity 1 (A), FASTA

>9GC3_1 Transcription factor tau 138 kDa subunit (chains A)
MVLTIYPDELVQIVSDKIASNKGKITLNQLWDISGKYFDLSDKKVKQFVLSCVILKKDIE
VYCDGAITTKNVTDIIGDANHSYSVGITEDSLWTLLTGYTKKESTIGNSAFELLLEVAKS
GEKGINTMDLAQVTGQDPRSVTGRIKKINHLLTSSQLIYKGHVVKQLKLKKFSHDGVDSN
PYINIRDHLATIVEVVKRSKNGIRQIIDLKRELKFDKEKRLSKAFIAAIAWLDEKEYLKK
VLVVSPKNPAIKIRCVKYVKDIPDSKGSPSFEYDSNSADEDSVSDSKAAFEDEDLVEGLD
NFNATDLLQNQGLVMEEKEDAVKNEVLLNRFYPLQNQTYDIADKSGLKGISTMDVVNRIT
GKEFQRAFTKSSEYYLESVDKQKENTGGYRLFRIYDFEGKKKFFRLFTAQNFQKLTNAED
EISVPKGFDELGKSRTDLKTLNEDNFVALNNTVRFTTDSDGQDIFFWHGELKIPPNSKKT
PNKNKRKRQVKNSTNASVAGNISNPKRIKLEQHVSTAQEPKSAEDSPSSNGGTVVKGKVV
NFGGFSARSLRSLQRQRAILKVMNTIGGVAYLREQFYESVSKYMGSTTTLDKKTVRGDVD
LMVESEKLGARTEPVSGRKIIFLPTVGEDAIQRYILKEKDSKKATFTDVIHDTEIYFFDQ
TEKNRFHRGKKSVERIRKFQNRQKNAKIKASDDAISKKSTSVNVSDGKIKRRDKKVSAGR
TTVVVENTKEDKTVYHAGTKDGVQALIRAVVVTKSIKNEIMWDKITKLFPNNSLDNLKKK
WTARRVRMGHSGWRAYVDKWKKMLVLAIKSEKISLRDVEELDLIKLLDIWTSFDEKEIKR
PLFLYKNYEENRKKFTLVRDDTLTHSGNDLAMSSMIQREISSLKKTYTRKISASTKDLSK
SQSDDYIRTVIRSILIESPSTTRNEIEALKNVGNESIDNVIMDMAKEKQIYLHGSKLECT
DTLPDILENRGNYKDFGVAFQYRCKVNELLEAGNAIVINQEPSDISSWVLIDLISGELLN
MDVIPMVRNVRPLTYTSRRFEIRTLTPPLIIYANSQTKLNTARKSAVKVPLGKPFSRLWV
NGSGSIRPNIWKQVVTMVVNEIIFHPGITLSRLQSRCREVLSLHEISEICKWLLERQVLI
TTDFDGYWVNHNWYSIYESTENLYFQSMSASAWSHPQFEKGGGSGGGSGGSAWSHPQFEK
S

Sequence of entity 2 (B), FASTA

>9GC3_2 Transcription factor tau 91 kDa subunit (chains B)
HHHHHHENLYFQGMAVIPAKKRGRPRKSVVAEVPYDSLASPVSENSGSKRPRRNASKKAV
ANFAQLVHAGRDDVINTTQVNNVDDTDDDDFVLNDEGDGEESDNVEIEFENELESTKNEV
ADLNSSGSGASVRPSGRRNTVQKLRLKKNSTKNMKSSSPGSSLGQKGRPIRLLKDLSSAR
DKIERIYGLNKEKLLLLAKVKEGFETSVFDFPFKNIQPDSPYFVCLDPPCKKESAYNKVI
GDKNRTVYHEINKTEFENMIKLRTKRLKLLIGEVDAEVSTGDKIEFPVLANGKRRGFIYN
VGGLVTDIAWLNIEENTDIGKDIQYLAVAVSQYMDEPLNEHLEMFDKEKHSSCIQIFKMN
TSTLHCVKVQTIVHSFGEVWDLKWHEGCHAPHLVGCLSFVSQEGTINFLEIIDNATDVHV
FKMCEKPSLTLSLADSLITTFDFLSPTTVVCGFKNGFVAEFDLTDPEVPSFYDQVHDSYI
LSVSTAYSDFEDTVVSTVAVDGYFYIFNPKDIATTKTTVSRFRGSNLVPVVYCPQIYSYI
YSDGASSLRAVPSRAAFAVHPLVSRETTITAIGVSRLHPMVLAGSADGSLIITNAARRLL
HGIKNSSATQKSLRLWKWDYSIKDDKYRIDSSYEVYPLTVNDVSKAKIDAHGINITCTKW
NETSAGGKCYAFSNSAGLLTLEYLS

Sequence of entity 3 (C), FASTA

>9GC3_3 Transcription factor tau 60 kDa subunit (chains C)
MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE
FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN
LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG
SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV
LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV
TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI
ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS
LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS
IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT
HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM

Sequence of entity 4 (D), FASTA

>9GC3_4 DNA (40-MER) (chains D)
GCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCATTTT

Sequence of entity 5 (E), FASTA

>9GC3_5 DNA (40-MER) (chains E)
AAAATGCCATCTCCTAGAATCGAACCAGGGTTTCATCGGC

Primary citation

Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC. Seifert-Davila, W., Chaban, A., Baudin, F. et al. Nucleic Acids Res (2025) 53. DOI 10.1093/nar/gkae1174 · PubMed

Other PDB entries of the same protein (UniProt P34111 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AIM 1.4 Å, Crystal structure of T138 central eWH domain
9GCK 3.7 Å, yeast TFIIIC TauA subcomplex bound to a tRNA gene
8FFZ 3.8 Å, TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

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