9GG5: Thromboxane A2 receptor-miniGq protein complex

Cryo-EM structure of Thromboxane A2 receptor-miniGq protein complex bound to U46619. Determined by electron microscopy at 3.26 Å resolution. Released 27 Aug 2025.

Method: Electron microscopy
Resolution: 3.26 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,944
Mol. weight: 112.72 kDa
Ligands: CLR, PUC
Released: 27 Aug 2025

Explore 9GG5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9GG5 contains 30 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-39	6	2
β-strand	69-75	7	2
β-strand	80-86	7	2
α-helix	96-100	5
β-strand	105-111	7	2
α-helix	118-129	12
α-helix	132-134	3
β-strand	138-144	7	2
α-helix	146-154	9
α-helix	160-162	3
α-helix	165-167	3
α-helix	178-179	2
α-helix	184-203	20
β-strand	211-214	4	2
α-helix	223-245	23

Chain B: 4 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-40	3
β-strand	47-49	3	3
β-strand	52	1	4
β-strand	58-63	6	5
β-strand	70-74	5	5
β-strand	78-82	5	5
β-strand	89-94	6	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	121-125	5	6
β-strand	135-139	5	6
β-strand	146-151	6	7
β-strand	156-161	6	7
β-strand	165-170	6	7
β-strand	175-181	7	7
β-strand	191-192	2	8
β-strand	198-202	5	8
β-strand	207-212	6	8
β-strand	218-223	6	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	262-264	3	9
α-helix	272	1
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	293-298	6	10
β-strand	304-309	6	10
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335	1	4
β-strand	336-339	4	3

Chain C: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	11-23	13
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain R: 11 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	19-24	6
α-helix	29-55	27
α-helix	62-90	29
α-helix	104-135	32
α-helix	147-164	18
α-helix	166-169	4
β-strand	174-177	4	1
β-strand	182-185	4	1
α-helix	191-227	37
α-helix	238-269	32
α-helix	284-305	22
α-helix	306-310	5
α-helix	313-319	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Thromboxane A2 receptor	R	protein	343	Homo sapiens	P21731 (AlphaFold model)
Engineered miniGq	A	protein	246	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>9GG5_1 Thromboxane A2 receptor (chains R)
MWPNGSSLGPCFRPTNITLEERRLIASPWFAASFCVVGLASNLLALSVLAGARQGGSHTR
SSFLTFLCGLVLTDFLGLLVTGTIVVSQHAALFEWHAVDPGCRLCRFMGVVMIFFGLSPL
LLGAAMASERYLGITRPFSRPAVASQRRAWATVGLVWAAALALGLLPLLGVGRYTVQYPG
SWCFLTLGAESGDVAFGLLFSMLGGLSVGLSFLLNTVSVATLCHVYHGQEAAQQRPRDSE
VEMMAQLLGIMVVASVCWLPLLVFIAQTVLRNPPAMSPAGQLSRTTEKELLIYLRVATWN
QILDPWVYILFRRAVLRRLQPRLSTRPRSLSLQPQLTQRSGLQ

Sequence of entity 2 (A), FASTA

>9GG5_2 Engineered miniGq (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 3 (B), FASTA

>9GG5_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>9GG5_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	4
PUC	(5Z)-7-{(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-oxabicyclo[2.2.1]hep…	C21 H34 O4	1

Primary citation

Structural and dynamic insights into agonist recognition and function of the thromboxane A 2 receptor. Krawinski, P., Matzov, D., Ryder, A. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-69844-9 · PubMed

Other PDB entries of the same protein (UniProt P21731 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6IIU 2.5 Å, Crystal structure of the human thromboxane A2 receptor bound to ramatroban
6IIV 3.0 Å, Crystal structure of the human thromboxane A2 receptor bound to daltroban
8XJN 3.06 Å, Cloprosetnol bound Thromboxane A2 receptor-Gq Protein Complex
8XJO 3.11 Å, U46619 bound Thromboxane A2 receptor-Gq Protein Complex
9GGG 3.25 Å, Cryo-EM structure of Thromboxane A2 receptor-miniGq Protein Complex bound to I-BOP

Browse structure collections

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