9GGG: Thromboxane A2 receptor-miniGq Protein Complex

Cryo-EM structure of Thromboxane A2 receptor-miniGq Protein Complex bound to I-BOP. Determined by electron microscopy at 3.25 Å resolution. Released 27 Aug 2025.

Method: Electron microscopy
Resolution: 3.25 Å
Organisms: Homo sapiens, synthetic construct
Chains: 5
Atoms: 8,656
Mol. weight: 140.23 kDa
Ligands: CLR, A1IK0
Released: 27 Aug 2025

Explore 9GGG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9GGG contains 35 α-helices and 67 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	2
β-strand	71-76	6	2
β-strand	79-84	6	2
α-helix	97-100	4
β-strand	105-109	5	2
β-strand	110-111	2	3
α-helix	118-129	12
α-helix	132-134	3
β-strand	138-140	3	2
β-strand	143-144	2	3
α-helix	146-155	10
α-helix	160-162	3
α-helix	165-169	5
α-helix	184-202	19
β-strand	211-212	2	2
β-strand	215	1	3
α-helix	224-245	22

Chain B: 4 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-40	3
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	70-74	5	5
β-strand	78-83	6	5
β-strand	88-94	7	5
β-strand	102-105	4	6
β-strand	111-115	5	6
β-strand	120-125	6	6
β-strand	134-140	7	6
β-strand	146-151	6	7
β-strand	157-161	5	7
β-strand	165-170	6	7
β-strand	175-181	7	7
β-strand	187	1	8
β-strand	191-192	2	8
β-strand	198-203	6	8
β-strand	207-212	6	8
β-strand	218-222	5	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	260-264	5	9
α-helix	272-273	2
β-strand	276	1	10
β-strand	284	1	11
β-strand	287-288	2	10
β-strand	294-297	4	10
β-strand	298	1	11
β-strand	304-307	4	10
β-strand	315-318	4	4
β-strand	329-331	3	4
β-strand	336-339	4	4

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-45	16
α-helix	56-58	3

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	19-24	6
α-helix	29-55	27
α-helix	61-89	29
α-helix	95-98	4
α-helix	103-135	33
α-helix	147-165	19
α-helix	166-169	4
β-strand	174-177	4	1
β-strand	182-185	4	1
α-helix	191-227	37
α-helix	239-270	32
α-helix	274	1
α-helix	281-283	3
α-helix	284-297	14
α-helix	299-305	7
α-helix	306-310	5
α-helix	313-319	7

Chain S: 4 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	12
β-strand	11-12	2	13
β-strand	17-25	9	12
α-helix	29-31	3
β-strand	33-39	7	14
β-strand	45-51	7	14
α-helix	53-55	3
β-strand	58-60	3	14
β-strand	68-73	6	12
β-strand	78-84	7	12
α-helix	88-90	3
β-strand	92-99	8	14
β-strand	110-111	2	14
β-strand	115-117	3	14
β-strand	118-119	2	13
β-strand	129	1	15
β-strand	134-135	2	16
β-strand	143-148	6	15
β-strand	154	1	17
β-strand	160	1	17
β-strand	162-167	6	16
β-strand	174-178	5	16
β-strand	182-183	2	16
β-strand	191-195	5	15
β-strand	199-204	6	15
β-strand	214-219	6	16
α-helix	225	1
β-strand	226-227	2	16
β-strand	231-233	3	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Thromboxane A2 receptor	R	protein	343	Homo sapiens	P21731 (AlphaFold model)
Engineered miniGq	A	protein	246	Homo sapiens	A0A804HIH4 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
Antibody fragment scFv16	S	protein	256	synthetic construct

Sequence of entity 1 (R), FASTA

>9GGG_1 Thromboxane A2 receptor (chains R)
MWPNGSSLGPCFRPTNITLEERRLIASPWFAASFCVVGLASNLLALSVLAGARQGGSHTR
SSFLTFLCGLVLTDFLGLLVTGTIVVSQHAALFEWHAVDPGCRLCRFMGVVMIFFGLSPL
LLGAAMASERYLGITRPFSRPAVASQRRAWATVGLVWAAALALGLLPLLGVGRYTVQYPG
SWCFLTLGAESGDVAFGLLFSMLGGLSVGLSFLLNTVSVATLCHVYHGQEAAQQRPRDSE
VEMMAQLLGIMVVASVCWLPLLVFIAQTVLRNPPAMSPAGQLSRTTEKELLIYLRVATWN
QILDPWVYILFRRAVLRRLQPRLSTRPRSLSLQPQLTQRSGLQ

Sequence of entity 2 (A), FASTA

>9GGG_2 Engineered miniGq (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 3 (B), FASTA

>9GGG_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>9GGG_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (S), FASTA

>9GGG_5 Antibody fragment scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	4
A1IK0	(~{Z})-7-[(1~{S},2~{R},3~{R},4~{R})-3-[(~{E},3~{R})-4-(4-iodanylphenoxy)-3-oxid…	C23 H29 I O5	1

Primary citation

Structural and dynamic insights into agonist recognition and function of the thromboxane A 2 receptor. Krawinski, P., Matzov, D., Ryder, A. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-69844-9 · PubMed

Other PDB entries of the same protein (UniProt P21731 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6IIU 2.5 Å, Crystal structure of the human thromboxane A2 receptor bound to ramatroban
6IIV 3.0 Å, Crystal structure of the human thromboxane A2 receptor bound to daltroban
8XJN 3.06 Å, Cloprosetnol bound Thromboxane A2 receptor-Gq Protein Complex
8XJO 3.11 Å, U46619 bound Thromboxane A2 receptor-Gq Protein Complex
9GG5 3.26 Å, Cryo-EM structure of Thromboxane A2 receptor-miniGq protein complex bound to U46619

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