Full-lenght Nedd4-2 E3 ubiquitin ligase in presence of Ca2+. Determined by electron microscopy at 4.11 Å resolution. Released 4 Jun 2025.
Explore 9GIM in 3D Show helices and sheets RCSB PDB PDBe
9GIM contains 27 α-helices and 31 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 20-30 | 11 | 1 |
| β-strand | 43-51 | 9 | 2 |
| β-strand | 57-63 | 7 | 2 |
| β-strand | 76-82 | 7 | 1 |
| β-strand | 87-95 | 9 | 2 |
| β-strand | 104-111 | 8 | 2 |
| β-strand | 129-132 | 4 | 1 |
| β-strand | 134 | 1 | 2 |
| β-strand | 145-151 | 7 | 1 |
| α-helix | 192-194 | 3 | |
| β-strand | 199-203 | 5 | 3 |
| β-strand | 209-213 | 5 | 3 |
| β-strand | 218-220 | 3 | 3 |
| α-helix | 223-225 | 3 | |
| α-helix | 233-251 | 19 | |
| β-strand | 253 | 1 | 4 |
| β-strand | 534-539 | 6 | 5 |
| β-strand | 543-548 | 6 | 5 |
| β-strand | 553-554 | 2 | 5 |
| α-helix | 570-573 | 4 | |
| α-helix | 577-587 | 11 | |
| β-strand | 598-602 | 5 | 6 |
| α-helix | 607-612 | 6 | |
| α-helix | 621-625 | 5 | |
| α-helix | 627 | 1 | |
| β-strand | 628-632 | 5 | 6 |
| α-helix | 643-655 | 13 | |
| α-helix | 658-660 | 3 | |
| β-strand | 663-665 | 3 | 7 |
| β-strand | 673-675 | 3 | 7 |
| α-helix | 679-682 | 4 | |
| α-helix | 686-703 | 18 | |
| α-helix | 713-719 | 7 | |
| α-helix | 726-729 | 4 | |
| α-helix | 734-745 | 12 | |
| α-helix | 748-750 | 3 | |
| β-strand | 754 | 1 | 8 |
| β-strand | 757-761 | 5 | 9 |
| β-strand | 764-768 | 5 | 9 |
| α-helix | 774-776 | 3 | |
| β-strand | 778 | 1 | 8 |
| α-helix | 784-792 | 9 | |
| α-helix | 793-797 | 5 | |
| α-helix | 801-812 | 12 | |
| α-helix | 817-819 | 3 | |
| α-helix | 828-833 | 6 | |
| β-strand | 835 | 1 | 4 |
| α-helix | 840-845 | 6 | |
| β-strand | 847-849 | 3 | 10 |
| α-helix | 858-869 | 12 | |
| α-helix | 872-883 | 12 | |
| α-helix | 893-895 | 3 | |
| β-strand | 897-898 | 2 | 11 |
| β-strand | 901-902 | 2 | 11 |
| β-strand | 905-908 | 4 | 10 |
| β-strand | 918-920 | 3 | 10 |
| β-strand | 925-927 | 3 | 10 |
| α-helix | 934-946 | 13 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like | A | protein | 959 | Homo sapiens | Q96PU5 (AlphaFold model) |
>9GIM_1 Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like (chains A) GLGSMATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENR ELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLP TEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEV VDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNN IRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPA SPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPP SVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHL IEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQ SFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEE RIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPN RFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMF NPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFY KMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSE IMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLG DVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAEL YGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
Structural basis of ubiquitin ligase Nedd4-2 autoinhibition and regulation by calcium and 14-3-3 proteins. Janosev, M., Kosek, D., Tekel, A. et al. Nat Commun (2025) 16:4875-4875. DOI 10.1038/s41467-025-60207-4 · PubMed
Other PDB entries of the same protein (UniProt Q96PU5 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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