9GUU: 30S mRNA delivery complex
30S mRNA delivery complex (consensus). Determined by electron microscopy at 2.5 Å resolution. Released 20 Nov 2024.
- Method
- Electron microscopy
- Resolution
- 2.5 Å
- Organisms
- Escherichia coli K-12, synthetic construct
- Chains
- 24
- Atoms
- 55,336
- Mol. weight
- 891.32 kDa
- Ligands
- MG
- Released
- 20 Nov 2024
Explore 9GUU in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9GUU contains 115 α-helices and 121 β-strands across 21 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain B: 4 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-15 | 11 | |
| β-strand | 23-31 | 9 | 1 |
| β-strand | 35-39 | 5 | 1 |
| β-strand | 46-49 | 4 | 1 |
| α-helix | 50-53 | 4 | |
| β-strand | 67-72 | 6 | 1 |
| β-strand | 84-85 | 2 | 1 |
| α-helix | 86-104 | 19 | |
| β-strand | 108 | 1 | 2 |
| β-strand | 111-113 | 3 | 3 |
| β-strand | 114-115 | 2 | 4 |
| β-strand | 119-123 | 5 | 4 |
| β-strand | 128-132 | 5 | 4 |
| β-strand | 148-151 | 4 | 3 |
| β-strand | 154 | 1 | 2 |
| β-strand | 156 | 1 | 5 |
| β-strand | 160 | 1 | 6 |
| α-helix | 161-163 | 3 | |
| β-strand | 165 | 1 | 6 |
| β-strand | 166-167 | 2 | 4 |
| β-strand | 168 | 1 | 5 |
Chain C: 11 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-12 | 7 | |
| β-strand | 16-19 | 4 | 7 |
| α-helix | 25-27 | 3 | |
| β-strand | 33-35 | 3 | 7 |
| β-strand | 38-40 | 3 | 7 |
| α-helix | 43-62 | 20 | |
| β-strand | 67-70 | 4 | 8 |
| α-helix | 77-86 | 10 | |
| β-strand | 90-92 | 3 | 8 |
| α-helix | 95-97 | 3 | |
| α-helix | 104-118 | 15 | |
| α-helix | 119-124 | 6 | |
| α-helix | 132-148 | 17 | |
| α-helix | 150-153 | 4 | |
| β-strand | 160-164 | 5 | 8 |
| α-helix | 170-179 | 10 | |
| β-strand | 183-187 | 5 | 8 |
| β-strand | 200-201 | 2 | 8 |
| α-helix | 207-226 | 20 | |
Chain D: 8 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-6 | 3 | |
| α-helix | 7-11 | 5 | |
| β-strand | 12 | 1 | 9 |
| β-strand | 16 | 1 | 9 |
| β-strand | 20 | 1 | 10 |
| α-helix | 29-46 | 18 | |
| β-strand | 52-58 | 7 | 10 |
| β-strand | 64-70 | 7 | 10 |
| α-helix | 73-77 | 5 | |
| α-helix | 82-95 | 14 | |
| β-strand | 99-105 | 7 | 10 |
| α-helix | 113-125 | 13 | |
| α-helix | 130-144 | 15 | |
| β-strand | 148-154 | 7 | 11 |
| α-helix | 157-159 | 3 | |
| β-strand | 165-171 | 7 | 11 |
| β-strand | 180-191 | 12 | 11 |
| β-strand | 194-206 | 13 | 11 |
Chain E: 15 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-15 | 8 | |
| α-helix | 28-31 | 4 | |
| α-helix | 37 | 1 | |
| α-helix | 46-48 | 3 | |
| α-helix | 50-65 | 16 | |
| α-helix | 69-81 | 13 | |
| α-helix | 86-96 | 11 | |
| β-strand | 97 | 1 | 12 |
| α-helix | 98-105 | 8 | |
| α-helix | 111-119 | 9 | |
| β-strand | 123-125 | 3 | 13 |
| β-strand | 128-129 | 2 | 13 |
| α-helix | 135 | 1 | |
| β-strand | 136 | 1 | 12 |
| α-helix | 137-138 | 2 | |
| β-strand | 141-145 | 5 | 13 |
| α-helix | 153-164 | 12 | |
| α-helix | 166-168 | 3 | |
| β-strand | 171-174 | 4 | 13 |
| β-strand | 179-182 | 4 | 13 |
| α-helix | 185-187 | 3 | |
| α-helix | 198-204 | 7 | |
Chain F: 6 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 12-22 | 11 | 14 |
| β-strand | 31-40 | 10 | 14 |
| β-strand | 45-53 | 9 | 14 |
| α-helix | 56-68 | 13 | |
| β-strand | 72-73 | 2 | 14 |
| β-strand | 77 | 1 | 15 |
| β-strand | 80 | 1 | 15 |
| β-strand | 85-88 | 4 | 16 |
| β-strand | 92-97 | 6 | 16 |
| α-helix | 99-100 | 2 | |
| β-strand | 105-106 | 2 | 16 |
| α-helix | 110-118 | 9 | |
| β-strand | 122 | 1 | 15 |
| β-strand | 123-129 | 7 | 16 |
| α-helix | 133-145 | 13 | |
| α-helix | 150-157 | 8 | |
| α-helix | 161-164 | 4 | |
Chain G: 3 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-10 | 9 | 17 |
| α-helix | 12-17 | 6 | |
| α-helix | 18-31 | 14 | |
| β-strand | 35-40 | 6 | 17 |
| β-strand | 44 | 1 | 17 |
| β-strand | 51 | 1 | 18 |
| β-strand | 54 | 1 | 18 |
| β-strand | 58 | 1 | 17 |
| β-strand | 61-66 | 6 | 17 |
| α-helix | 68-80 | 13 | |
| β-strand | 84-92 | 9 | 17 |
Chain H: 10 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-11 | 3 | |
| α-helix | 13-15 | 3 | |
| α-helix | 21-30 | 10 | |
| α-helix | 36-54 | 19 | |
| α-helix | 58-69 | 12 | |
| β-strand | 73-81 | 9 | 19 |
| β-strand | 83-90 | 8 | 19 |
| α-helix | 91-92 | 2 | |
| α-helix | 93-110 | 18 | |
| α-helix | 116-129 | 14 | |
| α-helix | 133-147 | 15 | |
| α-helix | 149-151 | 3 | |
Chain I: 4 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-19 | 14 | |
| β-strand | 24-28 | 5 | 20 |
| α-helix | 31-43 | 13 | |
| β-strand | 46-52 | 7 | 20 |
| β-strand | 58-63 | 6 | 20 |
| β-strand | 65 | 1 | 21 |
| β-strand | 70 | 1 | 21 |
| β-strand | 75-77 | 3 | 22 |
| β-strand | 80 | 1 | 23 |
| β-strand | 83 | 1 | 23 |
| β-strand | 86-87 | 2 | 22 |
| α-helix | 89-91 | 3 | |
| β-strand | 101-106 | 6 | 22 |
| β-strand | 109-112 | 4 | 22 |
| α-helix | 113-119 | 7 | |
| β-strand | 123-129 | 7 | 22 |
13 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| 16S ribosomal RNA | A | RNA | 1541 | Escherichia coli K-12 | |
| 30S ribosomal protein S1 | B | protein | 557 | Escherichia coli K-12 | P0AG67 (AlphaFold model) |
| 30S ribosomal protein S2 | C | protein | 241 | Escherichia coli K-12 | P0A7V0 (AlphaFold model) |
| Small ribosomal subunit protein uS3 | D | protein | 233 | Escherichia coli K-12 | P0A7V3 (AlphaFold model) |
| Small ribosomal subunit protein uS4 | E | protein | 206 | Escherichia coli K-12 | P0A7V8 (AlphaFold model) |
| 30S ribosomal protein S5 | F | protein | 156 | Escherichia coli K-12 | P0A7W1 |
| Small ribosomal subunit protein bS6 | G | protein | 131 | Escherichia coli K-12 | P02358 |
| 30S ribosomal protein S7 | H | protein | 156 | Escherichia coli K-12 | P02359 |
| 30S ribosomal protein S8 | I | protein | 130 | Escherichia coli K-12 | P0A7W7 |
| 30S ribosomal protein S9 | J | protein | 130 | Escherichia coli K-12 | P0A7X3 |
| 30S ribosomal protein S10 | K | protein | 103 | Escherichia coli K-12 | P0A7R5 |
| 30S ribosomal protein S11 | L | protein | 129 | Escherichia coli K-12 | P0A7R9 |
12 more molecules are not listed.
Sequence of entity 1 (A), FASTA
>9GUU_1 16S ribosomal RNA (chains A)
AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAA
GUCGAACGGUAACAGGAAGAAGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAA
UGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAAACGGUAGCUAAUACCGCAU
AACGUCGCAAGACCAAAGAGGGGUACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUG
GGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGA
GGAUGACCAGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGG
GGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCCGCGUGUAUGAAGAAGGCCU
UCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUU
GACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAG
GGUGCAAGCGUUAAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCA
GAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAUACUGGCAAGCUUGAGUCUC
GUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACC
GGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCA
AACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCC
CUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCGCCUGGGGAGUACGGCCGCA
AGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAU
UCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAG
AAUGUGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGA
AAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGC
CGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUC
AUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCG
ACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAAC
UCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGU
UCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGU
AGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAA
CAAGGUAACCGUAGGGGAACCUGCGGUUGGAUCACCUCCUU
Sequence of entity 2 (B), FASTA
>9GUU_2 30S ribosomal protein S1 (chains B)
MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKSESAIPAEQFKNAQGEL
EIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAYEDAETVTGVINGKVKGGF
TVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLDQKRNNVVVSRRAVIESENSA
ERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLLHITDMAWKRVKHPSEIVNVGDE
ITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPEGTKLTGRVTNLTDYGCFVEIEEGV
EGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLDIDEERRRISLGLKQCKANPWQQFAET
HNKGDRVEGKIKSITDFGIFIGLDGGIDGLVHLSDISWNVAGEEAVREYKKGDEIAAVVL
QVDAERERISLGVKQLAEDPFNNWVALNKKGAIVTGKVTAVDAKGATVELADGVEGYLRA
SEASRDRVEDATLVLSVGDEVEAKFTGVDRKNRAISLSVRAKDEADEKDAIATVNKQEDA
NFSNNAMAEAFKAAKGE
Sequence of entity 3 (C), FASTA
>9GUU_3 30S ribosomal protein S2 (chains C)
MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKI
ASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQ
SQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFVIDADHEHIAIKEANNLG
IPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEA
E
Sequence of entity 4 (D), FASTA
>9GUU_4 Small ribosomal subunit protein uS3 (chains D)
MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERP
AKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSI
TSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAEIARTEWYREGRVPLHTLRA
DIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK
Sequence of entity 5 (E), FASTA
>9GUU_5 Small ribosomal subunit protein uS4 (chains E)
MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQK
VRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSH
KAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEG
TFKRKPERSDLSADINEHLIVELYSK
Sequence of entity 6 (F), FASTA
>9GUU_6 30S ribosomal protein S5 (chains F)
ELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARR
NMINVALNNGTLQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYG
STNPINVVRATIDGLENMNSPEMVAAKRGKSVEEIL
Sequence of entity 7 (G), FASTA
>9GUU_7 Small ribosomal subunit protein bS6 (chains G)
MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYV
LMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANET
ADDADAGDSEE
Sequence of entity 8 (H), FASTA
>9GUU_8 30S ribosomal protein S7 (chains H)
MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELE
AFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRL
ANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW
Sequence of entity 9 (I), FASTA
>9GUU_9 30S ribosomal protein S8 (chains I)
MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELE
LTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAG
LGGEIICYVA
Sequence of entity 10 (J), FASTA
>9GUU_10 30S ribosomal protein S9 (chains J)
MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEK
LDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRK
ARRRPQFSKR
Sequence of entity 11 (K), FASTA
>9GUU_11 30S ribosomal protein S10 (chains K)
MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKD
ARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISLG
Sequence of entity 12 (L), FASTA
>9GUU_12 30S ribosomal protein S11 (chains L)
MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTP
FAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNG
CRPPKKRRV
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| MG | Magnesium ion | Mg | 120 |
Primary citation
Molecular basis of mRNA delivery to the bacterial ribosome. Webster, M.W., Chauvier, A., Rahil, H. et al. Science (2024) 386:eado8476-eado8476. DOI 10.1126/science.ado8476 · PubMed
Other PDB entries of the same protein (UniProt P0AG67 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 2BH8 1.9 Å, Combinatorial Protein 1b11
- 9IOT 2.7 Å, Cryo-EM structure of Escherichia coli hibernating ribosome with RNase I mutant
- 9GUP 2.8 Å, 30S mRNA delivery complex (open head)
- 9GUT 2.8 Å, 30S mRNA delivery complex (bS1 resolved)
- 4Q7J 2.9 Å, Complex structure of viral RNA polymerase
- 6H4N 3.0 Å, Structure of a hibernating 100S ribosome reveals an inactive conformation of the…
- 9GUV 3.0 Å, 30S mRNA delivery complex (closed-head)
- 6X7K 3.1 Å, Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3…
- 8URY 3.1 Å, Escherichia coli transcription-translation coupled complex class B (TTC-B) containing…
- 9GUQ 3.1 Å, 30S PIC (Pre-Initiation complex)
- 9GUW 3.1 Å, 30S-TEC (TEC in expressome position) Inactive state 2
- 6X6T 3.2 Å, Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1…
Browse structure collections
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