9IV2: PDB entry 9IV2

Identification, structure and agonist design of an androgen membrane receptor. Determined by electron microscopy at 3.53 Å resolution. Released 12 Feb 2025.

Method: Electron microscopy
Resolution: 3.53 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,732
Mol. weight: 153.5 kDa
Ligands: DHT
Released: 12 Feb 2025

Explore 9IV2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9IV2 contains 30 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	18-37	20
β-strand	41-45	5	9
α-helix	53-57	5
β-strand	207-214	8	9
β-strand	217-224	8	9
α-helix	234-238	5
β-strand	243-245	3	9
α-helix	267-277	11
β-strand	286-288	3	9
α-helix	294-301	8
α-helix	308-311	4
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-360	2	9
α-helix	371-390	20

Chain B: 7 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-10	7
α-helix	13-25	13
α-helix	30-33	4
α-helix	38-39	2
β-strand	49-51	3	1
β-strand	58-63	6	2
β-strand	70-74	5	2
β-strand	78-82	5	2
β-strand	89-94	6	2
β-strand	102-105	4	3
β-strand	111-115	5	3
β-strand	120-121	2	4
β-strand	122-125	4	3
β-strand	134-135	2	3
β-strand	139-140	2	4
β-strand	148-151	4	5
β-strand	156-160	5	5
β-strand	166-170	5	5
α-helix	172-174	3
β-strand	176-180	5	5
α-helix	186	1
β-strand	187-191	5	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	221	1	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	307-308	2	8
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	335-339	5	1

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	6-23	18
α-helix	30-43	14

Chain R: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	567-590	24
α-helix	600-623	24
α-helix	630-661	32
α-helix	672-680	9
α-helix	682-693	12
α-helix	706-707	2
α-helix	714-742	29
α-helix	757-769	13
α-helix	775-780	6
α-helix	789-796	8
α-helix	798-808	11
α-helix	811-826	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	344	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Gs protein alpha subunit	A	protein	361	Homo sapiens	P63092 (AlphaFold model)
Adhesion G-protein coupled receptor D1	R	protein	598	Homo sapiens	Q6QNK2 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>9IV2_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
GSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (G), FASTA

>9IV2_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (A), FASTA

>9IV2_3 Gs protein alpha subunit (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 4 (R), FASTA

>9IV2_4 Adhesion G-protein coupled receptor D1 (chains R)
HPIITNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPG
WIALSEDSAVVLSLIDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSS
HYRFPAHGQSFIQIPHEAFHRHAWSTVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLL
FATSHLISLEVSPPPTLSQNLSGSPLITVHLKHRLTRKQHSEATNSSNRVFVYCAFLDFS
SGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELARGHQVALSSISYVGCSL
SVLCLVATLVTFAVLSSVSTIRNQRYHIHANLSFAVLVAQVLLLISFRLEPGTTPCQVMA
VLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHRYYYGMGWGFPLLICIISLSFAMD
SYGTSNNCWLSLASGAIWAFVAPALFVIVVNIGILIAVTRVISQISADNYKIHGDPSAFK
LTAKAVAVLLPILGTSWVFGVLAVNGCAVVFQYMFATLNSLQGLFIFLFHCLLNSEVRAA
FKHKTKVWSLTSSSARTSNAKPFHSDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV

Ligands and cofactors

ID	Name	Formula	Copies
DHT	5-alpha-dihydrotestosterone	C19 H30 O2	1

Primary citation

Identification, structure, and agonist design of an androgen membrane receptor. Yang, Z., Ping, Y.Q., Wang, M.W. et al. Cell (2025) 188:1589. DOI 10.1016/j.cell.2025.01.006 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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