9IV6: HGPR4-Gs complex in pH7.0

Cryo-EM structure of hGPR4-Gs complex in pH7.0. Determined by electron microscopy at 2.71 Å resolution. Released 30 Jul 2025.

Method: Electron microscopy
Resolution: 2.71 Å
Organisms: Homo sapiens, synthetic construct
Chains: 5
Atoms: 8,594
Mol. weight: 160.83 kDa
Released: 30 Jul 2025

Explore 9IV6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9IV6 contains 31 α-helices and 66 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-38	25
β-strand	40-47	8	2
α-helix	53-63	11
β-strand	217-218	2	3
β-strand	221-224	4	2
β-strand	227-232	6	2
β-strand	233-234	2	3
α-helix	244-248	5
β-strand	253-259	7	2
α-helix	266-277	12
β-strand	286-292	7	2
α-helix	294-303	10
α-helix	313-315	3
α-helix	332-351	20
β-strand	359-363	5	2
α-helix	371-390	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	9-24	16
α-helix	30-34	5
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	88-94	7	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	121-125	5	6
β-strand	134-139	6	6
β-strand	146-153	8	7
β-strand	156-161	6	7
β-strand	166-170	5	7
β-strand	175-180	6	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	207-212	6	8
β-strand	218-223	6	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
α-helix	272	1
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304-308	5	10
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-339	4	4

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	14-23	10
α-helix	30-43	14
α-helix	53-55	3

Chain R: 14 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	20-46	27
α-helix	53-77	25
β-strand	82	1	1
α-helix	89-119	31
α-helix	126-128	3
α-helix	131-148	18
α-helix	151-153	3
β-strand	158-160	3	1
β-strand	166-168	3	1
α-helix	178-186	9
α-helix	187-191	5
α-helix	192-210	19
α-helix	216-253	38
α-helix	259-274	16
α-helix	276-283	8
α-helix	284-288	5
α-helix	291-303	13

Chain S: 3 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	12	1	12
β-strand	17-25	9	11
α-helix	29-31	3
β-strand	33-39	7	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-84	7	11
β-strand	92-99	8	13
β-strand	110-111	2	13
β-strand	115-117	3	13
β-strand	119	1	12
β-strand	140-141	2	14
β-strand	147	1	15
β-strand	155	1	16
β-strand	158-159	2	16
β-strand	160-161	2	14
β-strand	166	1	17
β-strand	172	1	17
β-strand	174-179	6	18
β-strand	186-190	5	18
β-strand	194-195	2	18
α-helix	196	1
β-strand	203-207	5	16
β-strand	212-216	5	16
β-strand	226-231	6	18
α-helix	237-238	2
β-strand	243-244	2	18
β-strand	245	1	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
G-protein coupled receptor 4	R	protein	362	Homo sapiens	P46093 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	361	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	377	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	59	Homo sapiens	P59768 (AlphaFold model)
scFv16	S	protein	285	synthetic construct

Sequence of entity 1 (R), FASTA

>9IV6_1 G-protein coupled receptor 4 (chains R)
MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLS
IADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVA
HPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAW
MNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPY
HVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKA
LHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPP
AQ

Sequence of entity 2 (A), FASTA

>9IV6_2 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 3 (B), FASTA

>9IV6_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSGVSGWRLFKKIS

Sequence of entity 4 (G), FASTA

>9IV6_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
NTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFRE

Sequence of entity 5 (S), FASTA

>9IV6_5 scFv16 (chains S)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSAGGGGSGGGGSGGGGSADIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLEL

Primary citation

Cryo-EM structure of hGPR4-Gs complex in pH7.0. Zhong, Y.N., Guo, L.L. To be published.

Other PDB entries of the same protein (UniProt P46093 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9JCO 2.36 Å, Cryo-EM structure of the proton-sensing GPCR (GPR4)-Gs protein complex at pH 6.5
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
8Z9P 2.5 Å, Cryo-EM structure of human GPR4-Gi complex
9JCP 2.55 Å, Cryo-EM structure of the proton-sensing GPCR (GPR4)-Gq protein complex at pH 7.4
9JCQ 2.59 Å, Cryo-EM structure of the proton-sensing GPCR (GPR4)-Gs protein complex at pH 7.4
9LMP 2.65 Å, Cryo-EM structure of antagonist-bounded inactive human GPR4
9JFV 2.67 Å, Cryo-EM structure of GPR4 complexed with miniGs/q in pH6.8
8Z3Q 2.76 Å, Cryo-EM structure of the hGPR4-Gs complex in pH7.6
9BIP 2.8 Å, Human proton sensing receptor GPR4 in complex with miniGs
9LGM 2.84 Å, Cryo-EM structure of GPR4 complexed with Gs in pH8.0
9JFX 2.87 Å, Cryo-EM structure of GPR4 complexed with miniGs/q in pH7.5
8Z3M 2.9 Å, Cryo-EM structure of the hGPR4-Gq complex in pH6.5

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