9JFT: GPR65

Cryo-EM structure of GPR65 complexed with miniGs in pH6.5. Determined by electron microscopy at 3.27 Å resolution. Released 13 Aug 2025.

Method: Electron microscopy
Resolution: 3.27 Å
Organisms: Homo sapiens, Lama glama
Chains: 5
Atoms: 7,996
Mol. weight: 133.07 kDa
Released: 13 Aug 2025

Explore 9JFT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9JFT contains 27 α-helices and 51 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	12-39	28
β-strand	41-46	6	1
α-helix	53-61	9
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	234-237	4
β-strand	243-249	7	1
α-helix	256-268	13
β-strand	276-282	7	1
α-helix	284-293	10
α-helix	298-301	4
α-helix	322-341	20
β-strand	349	1	1
β-strand	352-353	2	1
α-helix	361-380	20

Chain B: 2 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	30-34	5
β-strand	47-52	6	2
β-strand	58-62	5	3
β-strand	69-74	6	3
β-strand	78	1	3
β-strand	81-83	3	3
β-strand	88-91	4	3
β-strand	94	1	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	135-139	5	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-181	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
β-strand	276-278	3	8
β-strand	284-289	6	8
β-strand	293-298	6	8
β-strand	304-307	4	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	11-12	2	10
β-strand	17-25	9	9
β-strand	34-39	6	11
β-strand	45-51	7	11
α-helix	53-55	3
β-strand	59-60	2	11
α-helix	62-64	3
β-strand	68-73	6	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-95	4	11
β-strand	98	1	11
β-strand	122-124	3	11
β-strand	125-126	2	10

Chain R: 11 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	11-44	34
α-helix	48-75	28
α-helix	84-116	33
α-helix	128-151	24
β-strand	157	1	12
β-strand	168	1	12
α-helix	178-187	10
α-helix	188-193	6
α-helix	194-212	19
α-helix	218-253	36
α-helix	264-280	17
α-helix	282-291	10
α-helix	295-298	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	249	Homo sapiens	A0A804HIH4 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	157	Lama glama
Psychosine receptor	R	protein	358	Homo sapiens	Q8IYL9 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9JFT_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILH
GGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNR
LQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDAT
PEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRDIIQR
MHLRQYELL

Sequence of entity 2 (B), FASTA

>9JFT_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>9JFT_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>9JFT_4 Nanobody 35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAMQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWV
RQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCA
RCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH

Sequence of entity 5 (R), FASTA

>9JFT_5 Psychosine receptor (chains R)
MNSTCIEEQHDLDHYLFPIVYIFVIIVSIPANIGSLCVSFLQAKKESELGIYLFSLSLSD
LLYALTLPLWIDYTWNKDNWTFSPALCKGSAFLMYMNFYSSTAFLTCIAVDRYLAVVYPL
KFFFLRTRRFALMVSLSIWILETIFNAVMLWEDETVVEYCDAEKSNFTLCYDKYPLEKWQ
INLNLFRTCTGYAIPLVTILICNRKVYQAVRHNKATENKEKKRIIKLLVSITVIFVLCFT
PFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYD
MWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLEGRPLEVLFQGPGSSGHHHHHH

Primary citation

Structural basis of stepwise proton sensing-mediated GPCR activation. Yue, X., Peng, L., Liu, S. et al. Cell Res (2025) 35:423-436. DOI 10.1038/s41422-025-01092-w · PubMed

Other PDB entries of the same protein (UniProt A0A804HIH4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BIP 2.8 Å, Human proton sensing receptor GPR4 in complex with miniGs
9GGG 3.25 Å, Cryo-EM structure of Thromboxane A2 receptor-miniGq Protein Complex bound to I-BOP
9K25 3.31 Å, Cryo-EM structure of apo-P2Y purinoceptor 2-miniGq-Nb35 complex

Browse structure collections

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