9K27: Prolactin-releasing peptide receptor

PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex. Determined by electron microscopy at 2.68 Å resolution. Released 29 Jan 2025.

Method: Electron microscopy
Resolution: 2.68 Å
Organism: Homo sapiens
Chains: 6
Atoms: 9,213
Mol. weight: 161.06 kDa
Released: 29 Jan 2025

Explore 9K27 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9K27 contains 32 α-helices and 66 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	57-59	3
α-helix	60-87	28
α-helix	94-120	27
β-strand	126	1	1
α-helix	131-163	33
α-helix	169-172	4
α-helix	173-195	23
β-strand	198-199	2	2
β-strand	202	1	1
β-strand	209	1	1
β-strand	212-213	2	2
α-helix	220-230	11
α-helix	231-235	5
α-helix	236-253	18
α-helix	263-303	41
α-helix	313-332	20
α-helix	333-337	5
α-helix	340-351	12

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	9-24	16
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-52	6	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	166-168	3	6
β-strand	178-180	3	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	252-254	3	8
β-strand	259-262	4	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	296-298	3	9
β-strand	303-306	4	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335-339	5	3

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-22	13
α-helix	30-43	14
α-helix	53-55	3

Chain D: 1 helix, 27 β-strands

Element	Residues	Length	Sheet
β-strand	20-24	5	10
β-strand	27-29	3	11
β-strand	33-42	10	10
α-helix	46-48	3
β-strand	50-52	3	12
β-strand	55-56	2	11
β-strand	62-63	2	11
β-strand	66	1	13
β-strand	67-68	2	12
β-strand	77	1	13
β-strand	85-90	6	10
β-strand	95-103	9	10
β-strand	109-111	3	11
β-strand	114-116	3	12
β-strand	128	1	12
β-strand	132-136	5	11
β-strand	163-165	3	14
β-strand	173-177	5	15
β-strand	183	1	16
β-strand	189	1	16
β-strand	191-196	6	14
β-strand	203-207	5	14
β-strand	211-212	2	14
β-strand	220-224	5	15
β-strand	228-233	6	15
β-strand	243-248	6	14
β-strand	256	1	14
β-strand	260-263	4	14

Chain E: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-29	23
β-strand	35-40	6	17
α-helix	46-56	11
β-strand	184-189	6	17
β-strand	194-199	6	17
α-helix	207-209	3
α-helix	211-214	4
β-strand	219-225	7	17
α-helix	226-228	3
α-helix	234-237	4
α-helix	241-246	6
β-strand	262-268	7	17
α-helix	270-276	7
α-helix	282-285	4
α-helix	298-308	11
β-strand	318-322	5	17
α-helix	330-349	20

Chain F: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	18-22	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Prolactin-releasing peptide receptor	A	protein	370	Homo sapiens	P49683 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	357	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scfv16	D	protein	261	Homo sapiens
Guanine nucleotide-binding protein G(q) subunit alpha	E	protein	353	Homo sapiens	P50148 (AlphaFold model)
Prolactin-releasing peptide PrRP31	F	protein	32	Homo sapiens	P81277

Sequence of entity 1 (A), FASTA

>9K27_1 Prolactin-releasing peptide receptor (chains A)
MASSTTRGPRVSDLFSGLPPAVTTPANQSAEASAGNGSVAGADAPAVTPFQSLQLVHQLK
GLIVLLYSVVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCTACVPLTLAY
AFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISLRLSAYAV
LAIWALSAVLALPAAVHTYHVELKPHDVRLCEEFWGSQERQRQLYAWGLLLVTYLLPLLV
ILLSYVRVSVKLRNRVVPGCVTQSQADWDRARRRRTFCLLVVIVVVFAVCWLPLHVFNLL
RDLDPHAIDPYAFGLVQLLCHWLAMSSACYNPFIYAWLHDSFREELRKLLVAWPRKIAPH
GQNMTVSVVI

Sequence of entity 2 (B), FASTA

>9K27_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>9K27_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FFEKKFFCAIL

Sequence of entity 4 (D), FASTA

>9K27_4 scfv16 (chains D)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQGPAAA

Sequence of entity 5 (E), FASTA

>9K27_5 Guanine nucleotide-binding protein G(q) subunit alpha (chains E)
MGCTLSAEDKAAVERSKMIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 6 (F), FASTA

>9K27_6 Prolactin-releasing peptide PrRP31 (chains F)
SRTHRHSMEIRTPDINPAWYASRGIRPVGRFX

Primary citation

a peptide receptor complex structure. Wu, Z., Du, Y., Jun, X. To be published.

Other PDB entries of the same protein (UniProt P49683 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9V0X 2.66 Å, Cryo-EM structure of Gq-coupled PrRPR in complex with GUB08248
8ZPT 2.96 Å, Cryo-EM structure of prolactin-releasing peptide recognition with Gq
8ZPS 2.97 Å, Cryo-EM structure of prolactin-releasing peptide recognition with Gi
9K26 3.0 Å, PrRP31 bound prolactin-releasing peptide receptor coupled with Gi protein complex

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