9L0H: JR14a bound mouse C3aR

Structure of JR14a bound mouse C3aR in complex with Go. Determined by electron microscopy at 3.57 Å resolution. Released 26 Nov 2025.

Method: Electron microscopy
Resolution: 3.57 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,091
Mol. weight: 161.12 kDa
Ligands: A1D9A
Released: 26 Nov 2025

Explore 9L0H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9L0H contains 37 α-helices and 72 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	9-29	21
β-strand	34	1	2
β-strand	38-40	3	2
α-helix	46-52	7
β-strand	186-192	7	2
β-strand	195-201	7	2
α-helix	209-211	3
α-helix	213-216	4
β-strand	222-227	6	2
α-helix	246-255	10
α-helix	258-260	3
β-strand	265-270	6	2
α-helix	272-280	9
α-helix	297-310	14
β-strand	319	1	2
β-strand	322-323	2	2
α-helix	331-351	21

Chain B: 5 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-34	5
α-helix	38-39	2
β-strand	49-51	3	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100	1	5
β-strand	104-105	2	6
β-strand	111-114	4	6
β-strand	116	1	5
β-strand	120-125	6	6
β-strand	134-140	7	6
α-helix	145	1
β-strand	146-153	8	7
β-strand	156-161	6	7
β-strand	165-168	4	7
β-strand	178-180	3	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	217-222	6	8
β-strand	232-234	3	9
β-strand	240-244	5	9
β-strand	250-254	5	9
β-strand	259-264	6	9
α-helix	272	1
β-strand	273-275	3	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304-307	4	10
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain E: 14 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	21-50	30
α-helix	59-84	26
β-strand	88	1	1
α-helix	92-125	34
α-helix	129-132	4
α-helix	136-159	24
β-strand	170	1	1
α-helix	324-334	11
α-helix	335-339	5
α-helix	340-356	17
α-helix	367-395	29
α-helix	401-407	7
α-helix	408-410	3
α-helix	414-424	11
α-helix	425-429	5
α-helix	433-443	11

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-22	14
α-helix	30-43	14
α-helix	56-58	3

Chain H: 6 helices, 32 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	11
β-strand	6-7	2	12
β-strand	11-12	2	13
β-strand	17-23	7	12
β-strand	25	1	11
α-helix	29-31	3
β-strand	33-37	5	14
β-strand	39	1	15
β-strand	45	1	15
β-strand	49-51	3	14
α-helix	53-55	3
β-strand	58-60	3	14
β-strand	69-71	3	12
β-strand	78-84	7	12
α-helix	88-90	3
β-strand	92-94	3	16
β-strand	95-99	5	14
β-strand	111	1	14
β-strand	115-117	3	16
β-strand	118-119	2	13
α-helix	138-139	2
β-strand	141	1	17
β-strand	146	1	18
β-strand	155-159	5	19
β-strand	160	1	17
β-strand	166	1	20
β-strand	172	1	20
β-strand	174-179	6	21
β-strand	186-190	5	21
β-strand	194-195	2	21
β-strand	204-208	5	19
β-strand	211-216	6	19
α-helix	221-223	3
β-strand	226-231	6	21
α-helix	237	1
β-strand	239	1	21
β-strand	243	1	21
β-strand	244	1	18

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Muscarinic acetylcholine receptor M4,C3a anaphylatoxin chemotactic receptor	E	protein	533	Homo sapiens, Mus musculus	O09047 (AlphaFold model), P08173 (AlphaFold model)
Guanine nucleotide-binding protein G(o) subunit alpha	A	protein	250	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Antibody fragment - ScFv16	H	protein	248	Mus musculus

Sequence of entity 1 (E), FASTA

>9L0H_1 Muscarinic acetylcholine receptor M4,C3a anaphylatoxin chemotactic receptor (chains E)
MGKTIIALSYIFCLVFADYKDDDDAANFTPVNGSSGNQSVRLVTSSSLEVLFQGPGSESF
DADTNSTDLHSRPLFQPQDIASMVILGLTCLLGLLGNGLVLWVAGVKMKTTVNTVWFLHL
TLADFLCCLSLPFSLAHLILQGHWPYGLFLCKLIPSIIILNMFASVFLLTAISLDRCLIV
HKPIWCQNHRNVRTAFAICGCVWVVAFVMCVPVFVYRDLFIMDNRSICRYNFDSSRSYDY
WDYVYKLSLPESNSTDNSTAQLTGHMNDRSAPSSVQARDYFWTVTTALQSQPFLTSPEDS
FSLDSANQQPHYGGKPPNVLTAAVPSGFPVEDRKSNTLNADAFLSAHTELFPTASSGHLY
PYDFQGDYVDQFTYDNHVPTPLMAITITRLVVGFLVPFFIMVICYSLIVFRMRKTNFTKS
RNKTFRVAVAVVTVFFICWTPYHLVGVLLLITDPESSLGEAVMSWDHMSIALASANSCFN
PFLYALLGKDFRKKARQSIKGILEAAFSEELTHSTNCTQDKASSKRNNMSTDV

Sequence of entity 2 (A), FASTA

>9L0H_2 Guanine nucleotide-binding protein G(o) subunit alpha (chains A)
MGHHHHHHENLYFQGTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGADNSGKST
IVKQMKIIHGGSGGSGGTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIF
CVDLSDYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPL
TICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNAQVIFDAVTDIII
ANNLRGCGLY

Sequence of entity 3 (B), FASTA

>9L0H_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9L0H_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (H), FASTA

>9L0H_5 Antibody fragment - ScFv16 (chains H)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELK

Ligands and cofactors

ID	Name	Formula	Copies
A1D9A	(2~{S})-5-[bis(azanyl)methylideneamino]-2-[[5-[bis(4-chlorophenyl)methyl]-3-met…	C25 H26 Cl2 N4 O3 S	1

Primary citation

Molecular fingerprints of a convergent mechanism orchestrating diverse ligand recognition and species-specific pharmacology at the complement anaphylatoxin receptors. Mishra, S., Yadav, M.K., Dalal, A. et al. bioRxiv (2025). DOI 10.1101/2025.05.26.656101 · PubMed

Other PDB entries of the same protein (UniProt O09047 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9KV6 2.88 Å, Structure of mouse TLQP21 bound to mouse C3aR in complex with Go
9KV8 3.17 Å, Structure of human TLQP21 bound to mouse C3aR in complex with Go
9KZ8 3.3 Å, Structure of EP67 bound mouse C3aR in complex with Go
9UMJ 3.38 Å, Structure of EP54 bound mouse C3aR in complex with Go
9KZK 3.54 Å, Structure of mouse C3a bound mouse C3aR in complex with Go

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