9M0R: Neuropeptide FF receptor 1 complex with NPVF

Structure of neuropeptide FF receptor 1 complex with NPVF. Determined by electron microscopy at 2.47 Å resolution. Released 23 Jul 2025.

Method: Electron microscopy
Resolution: 2.47 Å
Organisms: Rattus norvegicus, Bos taurus, synthetic construct
Chains: 6
Atoms: 9,020
Mol. weight: 156.14 kDa
Released: 23 Jul 2025

Explore 9M0R in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9M0R contains 39 α-helices and 69 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	8
α-helix	46-53	8
β-strand	185-190	6	8
β-strand	195-200	6	8
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	8
α-helix	227-230	4
α-helix	232	1
β-strand	233	1	9
α-helix	234	1
β-strand	241	1	9
α-helix	242-254	13
β-strand	263-269	7	8
α-helix	271-277	7
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-323	5	8
α-helix	328-350	23

Chain B: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	2-24	23
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-40	3
β-strand	45-51	7	1
β-strand	60-63	4	2
β-strand	69-73	5	2
β-strand	78-83	6	2
β-strand	88-94	7	2
α-helix	95	1
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-151	6	4
β-strand	156-161	6	4
β-strand	165-170	6	4
β-strand	175-181	7	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	303-308	6	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-341	6	1

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	53-55	3

Chain R: 16 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	31-33	3	10
α-helix	40-69	30
α-helix	77-104	28
α-helix	114-146	33
α-helix	155-171	17
α-helix	175-177	3
β-strand	180-181	2	11
β-strand	184-185	2	12
β-strand	188-190	3	10
β-strand	196	1	10
β-strand	200-201	2	12
β-strand	204-205	2	11
α-helix	211-221	11
α-helix	222-226	5
α-helix	227-243	17
α-helix	265-281	17
α-helix	284-291	8
α-helix	292-296	5
α-helix	300-305	6
α-helix	306-310	5
α-helix	311-326	16
α-helix	327-331	5
α-helix	335-338	4

Chain S: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	13
β-strand	10-12	3	14
β-strand	17-25	9	13
α-helix	29-31	3
β-strand	33-39	7	14
β-strand	45-51	7	14
β-strand	58-60	3	14
β-strand	68-73	6	13
β-strand	78-84	7	13
α-helix	88-90	3
β-strand	92-99	8	14
β-strand	110-111	2	14
β-strand	115-119	5	14
β-strand	140-141	2	15
β-strand	146-148	3	16
β-strand	151	1	17
β-strand	153	1	17
β-strand	155-161	7	15
β-strand	166	1	18
β-strand	172	1	18
β-strand	174-179	6	16
β-strand	186-190	5	16
β-strand	194-195	2	16
α-helix	196	1
β-strand	203-207	5	15
β-strand	211-216	6	15
β-strand	225-231	7	16
β-strand	239	1	16
β-strand	243-246	4	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	366	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
neuropeptide VF	L	protein	9	synthetic construct
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Neuropeptide FF receptor 1	R	protein	363	Homo sapiens	Q9GZQ6 (AlphaFold model)
scFv16	S	protein	247	synthetic construct

Sequence of entity 1 (B), FASTA

>9M0R_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSG

Sequence of entity 2 (G), FASTA

>9M0R_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (L), FASTA

>9M0R_3 neuropeptide VF (chains L)
VPNLPQRFX

Sequence of entity 4 (A), FASTA

>9M0R_4 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 5 (R), FASTA

>9M0R_5 Neuropeptide FF receptor 1 (chains R)
MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVG
NTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSG
LVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTL
TVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIA
RKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSA
PQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAY
SER

Sequence of entity 6 (S), FASTA

>9M0R_6 scFv16 (chains S)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Primary citation

Structural basis of peptide recognition and modulation for neuropeptide FF receptors. Li, X., Zhang, H., Hu, W. et al. Cell Rep (2025) 44:116160-116160. DOI 10.1016/j.celrep.2025.116160 · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF
7DUQ 2.5 Å, Cryo-EM structure of the compound 2 and GLP-1-bound human GLP-1 receptor-Gs complex

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