9MOU: Actin, alpha cardiac muscle 1
Structure of native murine cardiac thin filament variant I79N in troponin T at pCa=5.8 in Ca2+-bound partially activated state (upper strand). Determined by electron microscopy at 5.6 Å resolution. Released 4 Jun 2025.
- Method
- Electron microscopy
- Resolution
- 5.6 Å
- Organism
- Mus musculus
- Chains
- 15
- Atoms
- 28,660
- Mol. weight
- 540.74 kDa
- Ligands
- CA, MG, ADP
- Released
- 4 Jun 2025
Explore 9MOU in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9MOU contains 174 α-helices and 149 β-strands across 15 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains A, B, C, D, E, F and G: 22 helices, 21 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-7 | 2 | |
| β-strand | 8 | 1 | 1 |
| β-strand | 11 | 1 | 2 |
| β-strand | 17-19 | 3 | 2 |
| β-strand | 21 | 1 | 1 |
| β-strand | 29-31 | 3 | 2 |
| β-strand | 35-38 | 4 | 3 |
| β-strand | 53-54 | 2 | 3 |
| α-helix | 55-59 | 5 | |
| α-helix | 62-64 | 3 | |
| β-strand | 65-68 | 4 | 3 |
| β-strand | 71 | 1 | 4 |
| β-strand | 76 | 1 | 4 |
| α-helix | 79-88 | 10 | |
| α-helix | 89-94 | 6 | |
| α-helix | 98-100 | 3 | |
| β-strand | 103-107 | 5 | 1 |
| α-helix | 113-125 | 13 | |
| β-strand | 131-136 | 6 | 1 |
| α-helix | 137-144 | 8 | |
| β-strand | 150-155 | 6 | 5 |
| β-strand | 160-166 | 7 | 5 |
| β-strand | 169-170 | 2 | 5 |
| α-helix | 172-174 | 3 | |
| β-strand | 176-178 | 3 | 5 |
| α-helix | 182-192 | 11 | |
| α-helix | 193-195 | 3 | |
| α-helix | 205-216 | 12 | |
| α-helix | 223-230 | 8 | |
| β-strand | 238-242 | 5 | 6 |
| β-strand | 245-250 | 6 | 6 |
| α-helix | 258-261 | 4 | |
| α-helix | 274-283 | 10 | |
| α-helix | 290-295 | 6 | |
| β-strand | 297-300 | 4 | 5 |
| α-helix | 302-305 | 4 | |
| α-helix | 309-320 | 12 | |
| β-strand | 329-330 | 2 | 5 |
| α-helix | 338-347 | 10 | |
| α-helix | 351-354 | 4 | |
| β-strand | 357-358 | 2 | 1 |
| α-helix | 359-365 | 7 | |
| α-helix | 367-369 | 3 | |
Chain H: 9 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-10 | 8 | |
| α-helix | 15-28 | 14 | |
| α-helix | 39-46 | 8 | |
| α-helix | 55-62 | 8 | |
| α-helix | 74-84 | 11 | |
| α-helix | 94-104 | 11 | |
| β-strand | 112 | 1 | 43 |
| α-helix | 114-124 | 11 | |
| α-helix | 130-140 | 11 | |
| β-strand | 148 | 1 | 43 |
| α-helix | 150-156 | 7 | |
Chain I: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 44-80 | 37 | |
| α-helix | 91-136 | 46 | |
| α-helix | 139-141 | 3 | |
| α-helix | 152-160 | 9 | |
Chain J: 2 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 201-215 | 15 | |
| α-helix | 226-271 | 46 | |
Chain K: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 87-174 | 88 | |
Chains L and M: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 65-282 | 218 | |
Chains N and O: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-54 | 52 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Actin, alpha cardiac muscle 1 | A, B, C, D, E, F, G | protein | 377 | Mus musculus | P68033 (AlphaFold model) |
| Troponin C, slow skeletal and cardiac muscles | H | protein | 161 | Mus musculus | P19123 (AlphaFold model) |
| Troponin I, cardiac muscle | I | protein | 211 | Mus musculus | P48787 (AlphaFold model) |
| Isoform 6 of Troponin T, cardiac muscle | J, K | protein | 288 | Mus musculus | P45379 (AlphaFold model) |
| Tropomyosin alpha-1 chain | L, M, N, O | protein | 284 | Mus musculus | P58771 |
Sequence of entity 1 (A, B, C, D, E, F, G), FASTA
>9MOU_1 Actin, alpha cardiac muscle 1 (chains A, B, C, D, E, F, G)
MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
LSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS
KQEYDEAGPSIVHRKCF
Sequence of entity 2 (H), FASTA
>9MOU_2 Troponin C, slow skeletal and cardiac muscles (chains H)
MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEM
IDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKMM
LQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE
Sequence of entity 3 (I), FASTA
>9MOU_3 Troponin I, cardiac muscle (chains I)
MADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQ
EMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVT
KNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKE
DIEKENREVGDWRKNIDALSGMEGRKKKFEG
Sequence of entity 4 (J, K), FASTA
>9MOU_4 Isoform 6 of Troponin T, cardiac muscle (chains J, K)
MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPM
EESKPKPRSFMPNLVPPKNPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEEL
VSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSN
MMHFGGYIQKQAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSI
YNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK
Sequence of entity 5 (L, M, N, O), FASTA
>9MOU_5 Tropomyosin alpha-1 chain (chains L, M, N, O)
MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKY
SEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAA
DESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAE
ERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAE
FAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 3 |
| MG | Magnesium ion | Mg | 7 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 7 |
Primary citation
The role of the troponin T interactions with actin in regulation of cardiac thin filament revealed by the troponin T pathogenic variant Ile79Asn. Risi, C.M., Landim-Vieira, M., Belknap, B. et al. J Mol Cell Cardiol (2025) 204:55-67. DOI 10.1016/j.yjmcc.2025.05.005 · PubMed
Other PDB entries of the same protein (UniProt P68033 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8ZBN 3.02 Å, Mouse MYH6 R404Q left ventricle ATM complex
- 8ZIU 3.54 Å, Mouse MYH6 R404Q left ventricle actin and myosin complex
- 9E2E 4.0 Å, The structure of the junction region of the wild-type murine native cardiac thin…
- 8ZBK 4.28 Å, Mouse left ventricle ATM complex
- 8ZIP 4.9 Å, Mouse left ventricle actin and myosin complex
- 9MOW 4.9 Å, Structure of native murine cardiac thin filament variant I79N in troponin T at pCa=5.8…
- 9MOP 5.0 Å, Structure of native murine cardiac thin filament variant I79N in troponin T at pCa=5.8…
- 9MOM 5.1 Å, Structure of native murine cardiac thin filament at pCa=5.8 in Ca2+-bound partially…
- 9MO7 5.2 Å, Structure of native murine cardiac thin filament at pCa=5.8 in Ca2+-bound fully…
- 9MO8 5.2 Å, Structure of native murine cardiac thin filament at pCa=5.8 in Ca2+-free state (upper…
- 9MOL 5.2 Å, Structure of native murine cardiac thin filament at pCa=5.8 in Ca2+-free tilted state…
- 9MON 5.2 Å, Structure of native murine cardiac thin filament at pCa=5.8 in Ca2+-bound fully…
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