9N29: HM4Di(GRANPA)

hM4Di(GRANPA) in complex with mGsI and diphenhydramine. Determined by electron microscopy at 2.6 Å resolution. Released 15 Jul 2026.

Method: Electron microscopy
Resolution: 2.6 Å
Organisms: Rattus norvegicus, Homo sapiens, Lama glama
Chains: 5
Atoms: 7,861
Mol. weight: 142.52 kDa
Ligands: 2PM
Released: 15 Jul 2026

Explore 9N29 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9N29 contains 39 α-helices and 47 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	2-26	25
β-strand	28-35	8	11
α-helix	37-39	3
α-helix	41-43	3
β-strand	65-71	7	11
β-strand	74-80	7	11
α-helix	91-97	7
β-strand	100-106	7	11
α-helix	113-125	13
β-strand	133-139	7	11
α-helix	141-150	10
α-helix	155-158	4
α-helix	161-164	4
α-helix	173-174	2
α-helix	179-198	20
β-strand	206-210	5	11
α-helix	218-238	21

Chain B: 3 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	43	1	1
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	135-139	5	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	1
β-strand	284-289	6	1
β-strand	294-298	5	1
β-strand	304-308	5	1
β-strand	317-320	4	2
β-strand	327-330	4	2
β-strand	336-339	4	2

Chain G: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	6-23	18
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	8
β-strand	11-12	2	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
α-helix	62-64	3
β-strand	68-73	6	8
β-strand	78-83	6	8
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-124	3	10
β-strand	125-126	2	9

Chain R: 17 helices, 0 β-strands

Element	Residues	Length
α-helix	33-59	27
α-helix	61-63	3
α-helix	67-80	14
α-helix	81-85	5
α-helix	86-95	10
α-helix	102-135	34
α-helix	140-143	4
α-helix	146-169	24
α-helix	171-174	4
α-helix	188-190	3
α-helix	193-200	8
α-helix	201-205	5
α-helix	206-221	16
α-helix	393-419	27
α-helix	432-449	18
α-helix	450-454	5
α-helix	458-463	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Homo sapiens	P59768 (AlphaFold model)
nanobody 35	N	protein	138	Lama glama
Guanine nucleotide-binding protein Gi chimera (mGsI)	A	protein	245	Homo sapiens
Muscarinic acetylcholine receptor M4	R	protein	478	Homo sapiens	P08173 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>9N29_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (G), FASTA

>9N29_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFC

Sequence of entity 3 (N), FASTA

>9N29_3 nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 4 (A), FASTA

>9N29_4 Guanine nucleotide-binding protein Gi chimera (mGsI) (chains A)
GCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGADNSGKSTIVKQMRILHGGSG
GSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEA
LNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPG
EDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDVTDIIIKMNLR
DCGLF

Sequence of entity 5 (R), FASTA

>9N29_5 Muscarinic acetylcholine receptor M4 (chains R)
ANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNR
QLQTVNNYFLFSLACADLIIGAFVMNLYTVYIIKGYWPLGAVVCDLWLALDCVVSNASVM
NLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVP
DNQCFIQFLSNPAVTFGTAIAGFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKT
LAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSA
TQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVP
ATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPFNVMVL
VNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Ligands and cofactors

ID	Name	Formula	Copies
2PM	N-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine	C17 H21 N O	1

Primary citation

Next-generation chemogenetic inhibition using a brain-permeant non-prescription agent. Devenish, S.O., Patel, S.D., Ussingkaer, L.V. et al. Signal Transduct Target Ther (2026) 11. DOI 10.1038/s41392-026-02865-4 · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF

Browse structure collections

About this viewer

MolViewer shows 9N29 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.