9P0K: Composite map of CXCL9-CXCR3-Gi-scFv16

Composite map of CXCL9-CXCR3-Gi-scFv16. Determined by electron microscopy at 3.5 Å resolution. Released 29 Apr 2026.

Method: Electron microscopy
Resolution: 3.5 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 9,430
Mol. weight: 206.86 kDa
Released: 29 Apr 2026

Explore 9P0K in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9P0K contains 37 α-helices and 70 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	46-80	35
α-helix	87-114	28
α-helix	120-153	34
α-helix	156-162	7
α-helix	167-188	22
β-strand	190-193	4	1
β-strand	202-205	4	1
α-helix	209-218	10
α-helix	219-225	7
α-helix	226-244	19
α-helix	249-282	34
α-helix	290-315	26
α-helix	316-320	5
α-helix	323-334	12

Chain B: 2 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	21-23	3
β-strand	24-30	7	2
β-strand	40-45	6	2
β-strand	50-52	3	2
α-helix	58-71	14

Chain C: 12 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	3
α-helix	46-53	8
β-strand	185-187	3	3
β-strand	190	1	4
β-strand	195	1	4
β-strand	196-200	5	3
α-helix	208-211	4
α-helix	212-215	4
β-strand	220-224	5	3
β-strand	226	1	5
α-helix	228-230	3
α-helix	242-254	13
α-helix	257-259	3
β-strand	263-268	6	3
β-strand	269	1	5
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-323	5	3
α-helix	324-326	3
α-helix	330-350	21

Chain D: 3 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	43	1	6
β-strand	47-51	5	7
β-strand	58-63	6	8
β-strand	69-74	6	8
β-strand	78-83	6	8
β-strand	88-94	7	8
β-strand	100-105	6	9
β-strand	111-116	6	9
β-strand	121-125	5	9
β-strand	134-139	6	9
β-strand	146-151	6	10
β-strand	156-161	6	10
β-strand	166-170	5	10
β-strand	175-180	6	10
β-strand	187-192	6	11
β-strand	198-203	6	11
β-strand	207-212	6	11
β-strand	217-222	6	11
β-strand	229-234	6	12
β-strand	240-245	6	12
β-strand	250-254	5	12
β-strand	259-264	6	12
β-strand	277-278	2	6
β-strand	284-288	5	6
β-strand	294-298	5	6
β-strand	304-307	4	6
β-strand	315-320	6	7
β-strand	327-331	5	7
β-strand	336-339	4	7

Chain E: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-24	16
α-helix	30-45	16

Chain F: 6 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	6-10	5	13
β-strand	14-15	2	14
β-strand	22-28	7	13
α-helix	32-34	3
β-strand	36-42	7	15
β-strand	48-54	7	15
α-helix	56-58	3
β-strand	61-63	3	15
β-strand	71-76	6	13
α-helix	77-79	3
β-strand	81-86	6	13
α-helix	91-93	3
β-strand	95-102	8	15
β-strand	113-114	2	15
β-strand	118-120	3	15
β-strand	121-122	2	14
β-strand	141-143	3	16
β-strand	150	1	17
β-strand	156-162	7	16
β-strand	167	1	18
β-strand	173	1	18
β-strand	175-180	6	19
β-strand	187-192	6	19
β-strand	195-196	2	19
β-strand	204-208	5	16
β-strand	212-217	6	16
α-helix	222-224	3
β-strand	227-232	6	19
α-helix	238	1
β-strand	239-240	2	19
β-strand	244-245	2	19
β-strand	248	1	17

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
C-X-C chemokine receptor type 3,GFP-like fluorescent chromoprotein FP506, related	A	protein	684	Homo sapiens	P49682 (AlphaFold model), U6GSR1 (AlphaFold model)
C-X-C motif chemokine 9	B	protein	103	Homo sapiens	Q07325 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	C	protein	376	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	D	protein	357	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E	protein	71	Homo sapiens	P59768
scFV16	F	protein	269	Mus musculus

Sequence of entity 1 (A), FASTA

>9P0K_1 C-X-C chemokine receptor type 3,GFP-like fluorescent chromoprotein FP506, related (chains A)
MKTIIALSYIFCLVFADYKDDDDKGSENLYFQSGSMVLEVSDHQVLNDAEVAALLENFSS
SYDYGENESDSCCTSPPCPQDFSLNFDRAFLPALYSLLFLLGLLGNGAVAAVLLSRRTAL
SSTDTFLLHLAVADTLLVLTLPLWAVDAAVQWVFGSGLCKVAGALFNINFYAGALLLACI
SFDRYLNIVHATQLYRRGPPARVTLTCLAVWGLCLLFALPDFIFLSAHHDERLNATHCQY
NFPQVGRTALRVLQLVAGFLLPLLVMAYCYAHILAVLLVSRGQRRLRAMRLVVVAVVAFA
LCWTPYHLVVLVDILMDLGALARNCGRESRVDVAKSVTSGLGYMHCCLNPLLYAFVGVKF
RERMWMLLLRLGCPNQRGLQRQPSSSRRDSSWSETSEASYSGLGNSLEVLFQGPMVSKGE
ELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLG
YGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
KGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQN
TPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSAWSHP
QFEKGGGSGGGSGGSAWSHPQFEK

Sequence of entity 2 (B), FASTA

>9P0K_2 C-X-C motif chemokine 9 (chains B)
TPVVRKGRCSCISTNQGTIHLQSLKDLKQFAPSPSCEKIEIIATLKNGVQTCLNPDSADV
KELIKKWEKQVSQKKKQKNGKKHQKKKVLKVRKSQRSRQKKTT

Sequence of entity 3 (C), FASTA

>9P0K_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains C)
MKKHHHHHHHHHHENLYFQGGSMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLL
LGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDS
ARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLND
LDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGV
TAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKI
KKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDA
VTDVIIKNNLKDCGLF

Sequence of entity 4 (D), FASTA

>9P0K_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
MHHHHHHHHGENLYFQGSSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (E), FASTA

>9P0K_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (F), FASTA

>9P0K_6 scFV16 (chains F)
AGSDVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGT
IYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTL
TVSSGGGSGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQ
RPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTF
GAGTKLELKAAAGNSLVPRGSHHHHHHHH

Primary citation

Molecular basis of CXC chemokine receptor 3 ligand multispecificity. Bouyssou, A., Sun, D., Zhou, T. et al. Sci Adv (2026) 12:eadz3767-eadz3767. DOI 10.1126/sciadv.adz3767 · PubMed

Other PDB entries of the same protein (UniProt P49682 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8HNM 2.94 Å, CXCR3-DNGi complex activated by VUF11222
9P0M 2.95 Å, Composite map of CXCL11-CXCR3-Gi-scFv16
8HNL 2.98 Å, CXCR3-DNGi complex activated by PS372424
8K2W 3.0 Å, Structure of CXCR3 complexed with antagonist AMG487
8HNK 3.01 Å, CXCR3-DNGi complex activated by CXCL11
8XYK 3.03 Å, Structure of CXCR3 in complex with VUF10661 and Go (Full map)
8Y0N 3.07 Å, Structure of CXCR3 in complex with VUF11418 and Go (Full map)
9P0L 3.1 Å, Composite map of CXCL10-CXCR3-Gi-scFv16
8XYI 3.16 Å, Structure of CXCR3 in complex with VUF10661 (Receptor-ligand focused map)
8K2X 3.2 Å, CXCR3-DNGi complex activated by CXCL10
8XXZ 3.3 Å, Structure of CXCR3 in the apo-state (Full map)
8Y0H 3.53 Å, Structure of CXCR3 in complex with VUF11418 (Receptor-ligand focused map)

Browse structure collections

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