9P0L: Composite map of CXCL10-CXCR3-Gi-scFv16

Composite map of CXCL10-CXCR3-Gi-scFv16. Determined by electron microscopy at 3.1 Å resolution. Released 29 Apr 2026.

Method: Electron microscopy
Resolution: 3.1 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 9,384
Mol. weight: 203.77 kDa
Released: 29 Apr 2026

Explore 9P0L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9P0L contains 41 α-helices and 69 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	46-79	34
α-helix	84-86	3
α-helix	87-104	18
α-helix	106-114	9
α-helix	121-153	33
α-helix	156-162	7
α-helix	167-189	23
β-strand	190-195	6	1
β-strand	200-205	6	1
α-helix	209-219	11
α-helix	220-225	6
α-helix	226-244	19
α-helix	249-282	34
α-helix	287-288	2
α-helix	290-314	25
α-helix	315-320	6
α-helix	323-335	13

Chain B: 2 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	15	1	2
α-helix	21-23	3
β-strand	24-30	7	2
β-strand	33	1	3
β-strand	36	1	3
β-strand	40-45	6	2
β-strand	51-54	4	2
α-helix	59-68	10

Chain C: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	33-40	8	4
α-helix	47-50	4
β-strand	185-191	7	4
β-strand	194-200	7	4
α-helix	208-210	3
α-helix	212-217	6
β-strand	220-226	7	4
α-helix	242-254	13
β-strand	263-269	7	4
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	4
α-helix	330-350	21

Chain D: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-24	20
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	120-125	6	7
β-strand	134-140	7	7
β-strand	146-151	6	8
β-strand	156-161	6	8
β-strand	165-170	6	8
β-strand	175-181	7	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	208-212	5	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	259-264	6	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	294-298	5	11
β-strand	303-308	6	11
β-strand	317-320	4	5
β-strand	327-330	4	5
β-strand	336-339	4	5

Chain E: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-24	16
α-helix	30-43	14
α-helix	49-51	3
α-helix	56-58	3

Chain F: 8 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	6-10	5	12
β-strand	13-15	3	13
β-strand	20-28	9	12
α-helix	32-34	3
β-strand	36-42	7	13
β-strand	48-54	7	13
α-helix	56-58	3
β-strand	61-63	3	13
β-strand	71-76	6	12
α-helix	77-79	3
β-strand	81-87	7	12
α-helix	91-93	3
β-strand	95-102	8	13
β-strand	113-114	2	13
β-strand	116	1	12
β-strand	118-122	5	13
β-strand	141-143	3	14
β-strand	146	1	15
α-helix	147-149	3
β-strand	150	1	16
β-strand	156-162	7	14
β-strand	167	1	17
β-strand	173	1	17
β-strand	175-180	6	15
β-strand	186-191	6	15
β-strand	195-196	2	15
α-helix	197	1
β-strand	204-208	5	14
β-strand	212-217	6	14
α-helix	222-224	3
β-strand	226-232	7	15
α-helix	238	1
β-strand	239-240	2	15
β-strand	244-246	3	15
β-strand	248	1	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
C-X-C chemokine receptor type 3,GFP-like fluorescent chromoprotein FP506, related	A	protein	684	Homo sapiens	P49682 (AlphaFold model), U6GSR1 (AlphaFold model)
C-X-C motif chemokine 10	B	protein	77	Homo sapiens	P02778 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	C	protein	376	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	D	protein	357	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E	protein	71	Homo sapiens	P59768
scFv16	F	protein	269	Mus musculus

Sequence of entity 1 (A), FASTA

>9P0L_1 C-X-C chemokine receptor type 3,GFP-like fluorescent chromoprotein FP506, related (chains A)
MKTIIALSYIFCLVFADYKDDDDKGSENLYFQSGSMVLEVSDHQVLNDAEVAALLENFSS
SYDYGENESDSCCTSPPCPQDFSLNFDRAFLPALYSLLFLLGLLGNGAVAAVLLSRRTAL
SSTDTFLLHLAVADTLLVLTLPLWAVDAAVQWVFGSGLCKVAGALFNINFYAGALLLACI
SFDRYLNIVHATQLYRRGPPARVTLTCLAVWGLCLLFALPDFIFLSAHHDERLNATHCQY
NFPQVGRTALRVLQLVAGFLLPLLVMAYCYAHILAVLLVSRGQRRLRAMRLVVVAVVAFA
LCWTPYHLVVLVDILMDLGALARNCGRESRVDVAKSVTSGLGYMHCCLNPLLYAFVGVKF
RERMWMLLLRLGCPNQRGLQRQPSSSRRDSSWSETSEASYSGLGNSLEVLFQGPMVSKGE
ELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLG
YGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
KGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQN
TPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSAWSHP
QFEKGGGSGGGSGGSAWSHPQFEK

Sequence of entity 2 (B), FASTA

>9P0L_2 C-X-C motif chemokine 10 (chains B)
VPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKA
IKNLLKAVSKERSKRSP

Sequence of entity 3 (C), FASTA

>9P0L_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains C)
MKKHHHHHHHHHHENLYFQGGSMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLL
LGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDS
ARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLND
LDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGV
TAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKI
KKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDA
VTDVIIKNNLKDCGLF

Sequence of entity 4 (D), FASTA

>9P0L_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
MHHHHHHHHGENLYFQGSSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (E), FASTA

>9P0L_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (F), FASTA

>9P0L_6 scFv16 (chains F)
AGSDVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGT
IYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTL
TVSSGGGSGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQ
RPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTF
GAGTKLELKAAAGNSLVPRGSHHHHHHHH

Primary citation

Molecular basis of CXC chemokine receptor 3 ligand multispecificity. Bouyssou, A., Sun, D., Zhou, T. et al. Sci Adv (2026) 12:eadz3767-eadz3767. DOI 10.1126/sciadv.adz3767 · PubMed

Other PDB entries of the same protein (UniProt P49682 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8HNM 2.94 Å, CXCR3-DNGi complex activated by VUF11222
9P0M 2.95 Å, Composite map of CXCL11-CXCR3-Gi-scFv16
8HNL 2.98 Å, CXCR3-DNGi complex activated by PS372424
8K2W 3.0 Å, Structure of CXCR3 complexed with antagonist AMG487
8HNK 3.01 Å, CXCR3-DNGi complex activated by CXCL11
8XYK 3.03 Å, Structure of CXCR3 in complex with VUF10661 and Go (Full map)
8Y0N 3.07 Å, Structure of CXCR3 in complex with VUF11418 and Go (Full map)
8XYI 3.16 Å, Structure of CXCR3 in complex with VUF10661 (Receptor-ligand focused map)
8K2X 3.2 Å, CXCR3-DNGi complex activated by CXCL10
8XXZ 3.3 Å, Structure of CXCR3 in the apo-state (Full map)
9P0K 3.5 Å, Composite map of CXCL9-CXCR3-Gi-scFv16
8Y0H 3.53 Å, Structure of CXCR3 in complex with VUF11418 (Receptor-ligand focused map)

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