9P92: PAC1nR-VIP-Gs complex

Cryo-EM structure of the PAC1nR-VIP-Gs complex. Determined by electron microscopy at 3.8 Å resolution. Released 19 Nov 2025.

Method: Electron microscopy
Resolution: 3.8 Å
Organisms: Homo sapiens, Lama glama
Chains: 6
Atoms: 8,758
Mol. weight: 165.99 kDa
Released: 19 Nov 2025

Explore 9P92 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9P92 contains 44 α-helices and 52 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	13-39	27
β-strand	41-47	7	1
α-helix	53-60	8
β-strand	208-214	7	1
β-strand	217-223	7	1
α-helix	234-238	5
β-strand	243-249	7	1
α-helix	265-278	14
α-helix	282-284	3
β-strand	287-292	6	1
α-helix	294-301	8
α-helix	313-316	4
α-helix	326-327	2
α-helix	332-350	19
β-strand	359	1	1
β-strand	362-363	2	1
α-helix	371-391	21

Chain B: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-34	5
α-helix	38-44	7
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
α-helix	84-86	3
β-strand	88-94	7	3
β-strand	102-105	4	4
β-strand	111-115	5	4
β-strand	120-125	6	4
β-strand	134-140	7	4
α-helix	145	1
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-254	6	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-23	14
α-helix	30-44	15
α-helix	45-47	3

Chain N: 4 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-11	2	10
β-strand	17-25	9	9
α-helix	29-31	3
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	58-60	3	11
α-helix	62-64	3
β-strand	69-73	5	9
α-helix	74-76	3
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	93-98	6	11
β-strand	118	1	11
β-strand	122-123	2	11
β-strand	124-125	2	10

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-27	26

Chain R: 21 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	26-32	7
α-helix	34-41	8
β-strand	58	1	12
β-strand	63	1	12
β-strand	71-77	7	13
α-helix	80-82	3
β-strand	112-119	8	13
β-strand	122-123	2	13
α-helix	124-125	2
α-helix	130-133	4
α-helix	146-176	31
α-helix	184-211	28
α-helix	224-256	33
α-helix	265-270	6
α-helix	271-275	5
α-helix	276-290	15
α-helix	303-336	34
α-helix	348-362	15
α-helix	364-366	3
α-helix	367-370	4
α-helix	380-382	3
α-helix	383-388	6
α-helix	389-391	3
α-helix	392-396	5
α-helix	397-402	6
α-helix	407-417	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody35	N	protein	138	Lama glama
Pituitary adenylate cyclase-activating polypeptide type I receptor	R	protein	483	Homo sapiens	P41586 (AlphaFold model)
Vasoactive intestinal peptide	P	protein	28	Homo sapiens	P01282

Sequence of entity 1 (A), FASTA

>9P92_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>9P92_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>9P92_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>9P92_4 Nanobody35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (R), FASTA

>9P92_5 Pituitary adenylate cyclase-activating polypeptide type I receptor (chains R)
DYKDDDDLEVLFQGPAMHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCW
KPAHVGEMVLVSCPELFRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTEDGWS
EPFPHYFDACGFDEYESETGDQDYYYLSVKALYTVGYSTSLVTLTTAMVILCRFRKLHCT
RNFIHMNLFVSFMLRAISVFIKDWILYAEQDSNHCFISTVECKAVMVFFHYCVVSNYFWL
FIEGLYLFTLLVETFFPERRYFYWYTIIGWGTPTVCVTVWATLRLYFDDTGCWDMNDSTA
LWWVIKGPVVGSIMVNFVLFIGIIVILVQKLQSPDMGGNESSIYLRLARSTLLLIPLFGI
HYTVFAFSPENVSKRERLVFELGLGSFQGFVVAVLYCFLNGEVQAEIKRKWRSWKVNRYF
AVDFKHRHPSLASSGVNGGTQLSILSKSSSQIRMSGLPADNLATPAGLEVLFQGPHHHHH
HHH

Sequence of entity 6 (P), FASTA

>9P92_6 Vasoactive intestinal peptide (chains P)
HSDAVFTDNYTRLRKQMAVKKYLNSILN

Primary citation

Structural basis of modified ligand selectivity from N-terminal PAC1R alternative splicing. Lu, J.J., Deganutti, G., Li, M. et al. Proc Natl Acad Sci U S A (2025) 122:e2521157122-e2521157122. DOI 10.1073/pnas.2521157122 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

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