9P94: PAC1sR-PACAP27-Gs complex

Cryo-EM structure of the PAC1sR-PACAP27-Gs complex. Determined by electron microscopy at 3.0 Å resolution. Released 19 Nov 2025.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Lama glama
Chains: 6
Atoms: 8,733
Mol. weight: 163.55 kDa
Released: 19 Nov 2025

Explore 9P94 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9P94 contains 35 α-helices and 54 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-39	27
β-strand	41-47	7	15
α-helix	49-51	3
α-helix	53-59	7
β-strand	208-213	6	15
β-strand	218-223	6	15
α-helix	234-238	5
β-strand	243-249	7	15
α-helix	265-277	13
β-strand	286-292	7	15
α-helix	294-303	10
α-helix	313-317	5
α-helix	326-327	2
α-helix	332-351	20
β-strand	359-362	4	15
α-helix	371-390	20

Chain B: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	58-61	4	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	88-94	7	2
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-151	6	4
β-strand	156-161	6	4
β-strand	165-170	6	4
β-strand	176-181	6	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	304-308	5	7
β-strand	315	1	8
β-strand	319-320	2	1
β-strand	327-330	4	1
β-strand	331	1	8
β-strand	336-339	4	1

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-24	16
α-helix	30-42	13

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	9
β-strand	11	1	10
β-strand	18-25	8	9
α-helix	29-31	3
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	58-60	3	11
α-helix	62-64	3
β-strand	68-73	6	9
β-strand	78-83	6	9
α-helix	88-90	3
β-strand	92-95	4	11
β-strand	98	1	11
β-strand	122-124	3	11
β-strand	125	1	10

Chain P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	2-26	25

Chain R: 16 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	29-42	14
β-strand	58	1	12
β-strand	63	1	12
β-strand	72-75	4	13
α-helix	79-81	3
β-strand	114-117	4	13
β-strand	118-119	2	14
β-strand	122-123	2	14
α-helix	130-134	5
α-helix	147-177	31
α-helix	184-207	24
α-helix	208-212	5
α-helix	224-256	33
α-helix	265-290	26
α-helix	303-335	33
α-helix	347-362	16
α-helix	364-367	4
α-helix	378-382	5
α-helix	383-388	6
α-helix	392-397	6
α-helix	398-402	5
α-helix	405-417	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody35	N	protein	138	Lama glama
Pituitary adenylate cyclase-activating polypeptide	P	protein	27	Homo sapiens	P18509 (AlphaFold model)
Isoform S of Pituitary adenylate cyclase-activating polypeptide type I receptor	R	protein	462	Homo sapiens	P41586 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092

Sequence of entity 1 (B), FASTA

>9P94_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (G), FASTA

>9P94_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (N), FASTA

>9P94_3 Nanobody35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 4 (P), FASTA

>9P94_4 Pituitary adenylate cyclase-activating polypeptide (chains P)
HSDGIFTDSYSRYRKQMAVKKYLAAVL

Sequence of entity 5 (R), FASTA

>9P94_5 Isoform S of Pituitary adenylate cyclase-activating polypeptide type I receptor (chains R)
DYKDDDDLEVLFQGPAMHSDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWDNITCW
KPAHVGEMVLVSCPELFRIFNPDQDMGVVSRNCTEDGWSEPFPHYFDACGFDEYESETGD
QDYYYLSVKALYTVGYSTSLVTLTTAMVILCRFRKLHCTRNFIHMNLFVSFMLRAISVFI
KDWILYAEQDSNHCFISTVECKAVMVFFHYCVVSNYFWLFIEGLYLFTLLVETFFPERRY
FYWYTIIGWGTPTVCVTVWATLRLYFDDTGCWDMNDSTALWWVIKGPVVGSIMVNFVLFI
GIIVILVQKLQSPDMGGNESSIYLRLARSTLLLIPLFGIHYTVFAFSPENVSKRERLVFE
LGLGSFQGFVVAVLYCFLNGEVQAEIKRKWRSWKVNRYFAVDFKHRHPSLASSGVNGGTQ
LSILSKSSSQIRMSGLPADNLATPAGLEVLFQGPHHHHHHHH

Sequence of entity 6 (A), FASTA

>9P94_6 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Primary citation

Structural basis of modified ligand selectivity from N-terminal PAC1R alternative splicing. Lu, J.J., Deganutti, G., Li, M. et al. Proc Natl Acad Sci U S A (2025) 122:e2521157122-e2521157122. DOI 10.1073/pnas.2521157122 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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