9PA1: PCP bound kappa-opioid receptor

PCP bound kappa-opioid receptor in complex with Gi1. Determined by electron microscopy at 2.63 Å resolution. Released 1 Jul 2026.

Method: Electron microscopy
Resolution: 2.63 Å
Organisms: Escherichia coli, Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,716
Mol. weight: 175.13 kDa
Ligands: 1PC
Released: 1 Jul 2026

Explore 9PA1 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9PA1 contains 37 α-helices and 64 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	59-86	28
α-helix	93-108	16
α-helix	112-119	8
α-helix	128-161	34
α-helix	163-169	7
α-helix	172-195	24
β-strand	198-199	2	1
β-strand	210-211	2	1
α-helix	219-231	13
α-helix	232-236	5
α-helix	237-253	17
α-helix	264-297	34
α-helix	302-304	3
α-helix	306-330	25

Chain B: 11 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	16
α-helix	46-54	9
β-strand	185-190	6	16
β-strand	195-200	6	16
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	16
α-helix	227-230	4
β-strand	233	1	17
β-strand	241	1	17
α-helix	242-254	13
α-helix	257-259	3
β-strand	263-269	7	16
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-323	5	16
α-helix	328-351	24

Chain C: 6 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-40	3
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
α-helix	186	1
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-44	15
α-helix	56-58	3

Chain E: 5 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	11-12	2	10
β-strand	18-25	8	9
α-helix	29-31	3
β-strand	33-39	7	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	69-73	5	9
β-strand	78-83	6	9
α-helix	88-90	3
β-strand	93-99	7	11
β-strand	110-111	2	11
β-strand	115-116	2	11
β-strand	118-119	2	10
β-strand	128-129	2	12
β-strand	135	1	13
β-strand	143-149	7	12
β-strand	154	1	14
β-strand	160	1	14
β-strand	162-167	6	15
β-strand	174-177	4	15
β-strand	183	1	15
α-helix	184	1
β-strand	191-196	6	12
β-strand	199-204	6	12
α-helix	209-211	3
β-strand	214-219	6	15
β-strand	228	1	12
α-helix	230	1
β-strand	231-232	2	15
β-strand	233	1	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Soluble cytochrome b562,Kappa-type opioid receptor chimera	A	protein	476	Escherichia coli, Homo sapiens	P41145 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	358	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	80	Homo sapiens	P59768 (AlphaFold model)
scFv16	E	protein	305	Mus musculus
Guanine nucleotide-binding protein G(i) subunit alpha-1	B	protein	354	Homo sapiens	P63096 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9PA1_1 Soluble cytochrome b562,Kappa-type opioid receptor chimera (chains A)
MKTIIALSYIFCLVFADYKDDDDAKLQTMHHHHHHHHHHENLYFQGGTTMADLEDNWETL
NDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVG
QIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLGSLEVLFQGPGSGSGISPAIPVII
TAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSTVYLMN
SWPFGDVLCKIVLSIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPLKAKIINICI
WLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDYSWWDLFMKICVFIFAFVIPVLII
IVCYTLMILRLKSVRLLSGSREKDRNLRRITRLVLVVVAVFVVCWTPIHIFILVEALGST
SHSTAALSSYYFCIALGYTNSSLNPILYAFLDENFKRCFRDFCFPLKMRMERQSTS

Sequence of entity 2 (C), FASTA

>9PA1_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (D), FASTA

>9PA1_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSA

Sequence of entity 4 (E), FASTA

>9PA1_4 scFv16 (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFADVQLVESGGGLVQPGGSRKLSC
SASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQ
MTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELKAAALEVLFQGPHHH
HHHHH

Sequence of entity 5 (B), FASTA

>9PA1_5 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains B)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Ligands and cofactors

ID	Name	Formula	Copies
1PC	1-(phenyl-1-cyclohexyl)piperidine	C17 H25 N	1

Primary citation

Structural basis of opioid receptor activation by PCP and ketamine. Jiang, Q., Han, J., Fine, E.J. et al. Nat Struct Mol Biol (2026) 33:1341-1353. DOI 10.1038/s41594-026-01839-y · PubMed

Other PDB entries of the same protein (UniProt P41145 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7UL2 2.4 Å, CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
9PA4 2.5 Å, (S)-ketamine bound kappa-opioid receptor in complex with Gi1
8FEG 2.54 Å, CryoEM structure of Kappa Opioid Receptor bound to a semi-peptide and Gi1
9XDR 2.58 Å, Cryo-EM structure of asimadoline-BMS-986187-bound KOR-Gi1 complex
9ZZO 2.6 Å, MP1104-bound Kappa Opioid Receptor in complex with beta-arrestin1
8DZR 2.61 Å, GR89,696 bound Kappa Opioid Receptor in complex with gustducin
8DZS 2.65 Å, GR89,696 bound Kappa Opioid Receptor in complex with Gz
7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
8DZP 2.71 Å, momSalB bound Kappa Opioid Receptor in complex with Gi1
9V6O 2.76 Å, Cryo-EM structure of human kappa opioid receptor - G protein signaling complex bound…

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