9PA2: PCP bound mu-opioid receptor

PCP bound mu-opioid receptor in complex with Gi1. Determined by electron microscopy at 2.86 Å resolution. Released 1 Jul 2026.

Method: Electron microscopy
Resolution: 2.86 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,673
Mol. weight: 147.78 kDa
Ligands: 1PC
Released: 1 Jul 2026

Explore 9PA2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9PA2 contains 34 α-helices and 64 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34	1	1
β-strand	37-40	4	1
α-helix	44-53	10
β-strand	185-191	7	1
β-strand	194-200	7	1
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	1
α-helix	227-229	3
α-helix	232	1
β-strand	233	1	2
α-helix	234	1
β-strand	241	1	2
α-helix	244-254	11
β-strand	263-269	7	1
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	1
α-helix	328-351	24

Chain B: 5 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
α-helix	95	1
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187	1	7
β-strand	191-192	2	8
β-strand	198-202	5	8
β-strand	203	1	7
β-strand	207-212	6	8
β-strand	218-223	6	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
α-helix	272	1
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304-308	5	10
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	27-29	3
α-helix	30-43	14

Chain E: 3 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11-12	2	12
β-strand	17-25	9	11
α-helix	29-31	3
β-strand	33-39	7	13
β-strand	45-51	7	13
α-helix	53-55	3
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-84	7	11
β-strand	92-99	8	13
β-strand	110-111	2	13
β-strand	115-117	3	13
β-strand	118-119	2	12
β-strand	142	1	14
β-strand	147-149	3	15
β-strand	156-161	6	14
β-strand	167	1	16
β-strand	173	1	16
β-strand	175-180	6	15
β-strand	187-191	5	15
β-strand	195-196	2	15
α-helix	197	1
β-strand	204-209	6	14
β-strand	212-217	6	14
β-strand	226-232	7	15
β-strand	240	1	15
β-strand	244-247	4	15

Chain R: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	68-97	30
α-helix	104-132	29
α-helix	139-172	34
α-helix	174-180	7
α-helix	183-206	24
α-helix	229-239	11
α-helix	240-244	5
α-helix	245-263	19
α-helix	271-307	37
α-helix	314-340	27
α-helix	343-349	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Mu-type opioid receptor	R	protein	309	Homo sapiens	P35372 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
ScFv16 protein	E	protein	251	Mus musculus

Sequence of entity 1 (R), FASTA

>9PA2_1 Mu-type opioid receptor (chains R)
GRDSLCPPTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLA
LADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIWTLCTMSVDRYIAVCH
PVKALDFRTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENL
LKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIV
CWTPIHIYVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFC
IPTSSNIEQ

Sequence of entity 2 (A), FASTA

>9PA2_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (B), FASTA

>9PA2_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
GSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>9PA2_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (E), FASTA

>9PA2_5 ScFv16 protein (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAA

Ligands and cofactors

ID	Name	Formula	Copies
1PC	1-(phenyl-1-cyclohexyl)piperidine	C17 H25 N	1

Primary citation

Structural basis of opioid receptor activation by PCP and ketamine. Jiang, Q., Han, J., Fine, E.J. et al. Nat Struct Mol Biol (2026) 33:1341-1353. DOI 10.1038/s41594-026-01839-y · PubMed

Other PDB entries of the same protein (UniProt P35372 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8Y72 2.65 Å, positive allosteric modulator(BMS986122)-bound mu-opioid receptor-Gi complex
9PY4 2.78 Å, Primed-state loperamide-mu opioid receptor-Gi GDPbS complex (rebound)
8EFO 2.8 Å, PZM21-bound mu-opioid receptor-Gi complex
9WST 2.8 Å, Cryo-EM structure of DAMGO-muOR-Gz-scFv16 complex
8Y73 2.84 Å, positive allosteric modulator(MPAM-15)-bound mu-opioid receptor-Gi complex
9PA5 2.92 Å, (S)-ketamine bound mu-opioid receptor in complex with Gi1
23SO 2.95 Å, Cryo-EM structure of the cebranopadol-mu opioid receptor-Gi complex
9VJT 2.97 Å, Cryo-EM structure of MST-OR complex
8K9K 2.98 Å, Full agonist-bound mu-type opioid receptor-G protein complex
10TM 3.0 Å, Cryo-EM structure of MOR bound with DAMGO
9PUD 3.0 Å, Cryo-EM structure of MOR-Gi1 complex protein bound with DAMGO
9PXY 3.0 Å, Primed-state naloxone-mu opioid receptor-Gi GDPbS complex (rebound)

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