9PUD: MOR-Gi1 complex protein

Cryo-EM structure of MOR-Gi1 complex protein bound with DAMGO. Determined by electron microscopy at 3.0 Å resolution. Released 27 May 2026.

Method: Electron microscopy
Resolution: 3.0 Å
Organism: Homo sapiens
Chains: 10
Atoms: 14,284
Mol. weight: 262.69 kDa
Ligands: CLR
Released: 27 May 2026

Explore 9PUD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9PUD contains 62 α-helices and 76 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and C: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	10-30	21
β-strand	33-39	7	1
α-helix	46-52	7
β-strand	185-190	6	1
β-strand	195-200	6	1
α-helix	208-211	4
β-strand	220-226	7	1
α-helix	227-231	5
α-helix	232-234	3
α-helix	242-254	13
β-strand	263-269	7	1
α-helix	271-280	10
α-helix	296-309	14
β-strand	319-322	4	1
α-helix	331-350	20

Chains B and D: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	189-192	4	6
β-strand	198-202	5	6
β-strand	208-212	5	6
β-strand	218-220	3	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250	1	7
β-strand	253-254	2	7
β-strand	259-260	2	7
β-strand	264	1	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	317-320	4	2
β-strand	327-330	4	2
β-strand	336-339	4	2

Chains E and G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	6-23	18
α-helix	30-43	14
α-helix	53-55	3
α-helix	56-58	3

Chain F: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	68-94	27
α-helix	95-99	5
α-helix	104-120	17
α-helix	123-132	10
α-helix	138-172	35
α-helix	174-180	7
α-helix	183-196	14
α-helix	198-207	10
β-strand	208-213	6	18
β-strand	216-221	6	18
α-helix	227-238	12
α-helix	239-244	6
α-helix	245-261	17
α-helix	265-267	3
α-helix	273-305	33
α-helix	314-334	21
α-helix	335-340	6

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	68-94	27
α-helix	95-99	5
α-helix	104-120	17
α-helix	123-132	10
α-helix	138-172	35
α-helix	174-180	7
α-helix	183-196	14
α-helix	198-207	10
β-strand	208-213	6	9
β-strand	216-221	6	9
α-helix	227-238	12
α-helix	239-244	6
α-helix	245-261	17
α-helix	265-267	3
α-helix	273-305	33
α-helix	314-335	22
α-helix	336-340	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1	A, C	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B, D	protein	342	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E, G	protein	80	Homo sapiens	P59768 (AlphaFold model)
Mu-type opioid receptor	F, R	protein	388	Homo sapiens	P35372 (AlphaFold model)
Damgo	H, S	protein	5	Homo sapiens

Sequence of entity 1 (A, C), FASTA

>9PUD_1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A, C)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 2 (B, D), FASTA

>9PUD_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B, D)
MSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKI
YAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLD
NICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTT
TFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNG
NAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWD
ALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (E, G), FASTA

>9PUD_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E, G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSA

Sequence of entity 4 (F, R), FASTA

>9PUD_4 Mu-type opioid receptor (chains F, R)
MDSSAAPTNASNCTDALAYSSCSPAPSPGSWVNLSHLDGNLSDPCGPNRTDLGGRDSLCP
PTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALAT
STLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIWTLCTMSVDRYIAVCHPVKALDF
RTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFI
FAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHI
YVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSNI
EQQNSTRIRQNTRDHPSTANTVDRTNHQ

Sequence of entity 5 (H, S), FASTA

>9PUD_5 DAMGO (chains H, S)
YAGFX

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	4

Primary citation

cryo-EM structure of MOR-Gi1 complex protein bound with DAMGO. Zhang, H., Wacker, D., Zilberg, G. et al. To be published.

Other PDB entries of the same protein (UniProt P63096 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
3UMR 2.04 Å, Crystal structure of the G202D mutant of human G-alpha-i1
9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
2OM2 2.2 Å, Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14
8YN9 2.3 Å, Cryo-EM structure of histamine H4 receptor in complex with histamine and Gi
9HYI 2.3 Å, DP81-bound serotonin 5-HT1A receptor - Gi Protein Complex
9O36 2.3 Å, CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
8XXV 2.33 Å, Cryo-EM Structure of the Prostaglandin D2 Receptor 2-indomethacin Coupled to G Protein
3UMS 2.34 Å, Crystal structure of the G202A mutant of human G-alpha-i1
3ONW 2.38 Å, Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…

Browse structure collections

About this viewer

MolViewer shows 9PUD directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.