9RLG: LolCDE complex with LolB lipoprotein

Name: PDB 9RLG: LolCDE complex with LolB lipoprotein
Published: 2026-01-21
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: protein transport

LolCDE complex with LolB lipoprotein. Determined by electron microscopy at 3.09 Å resolution. Released 21 Jan 2026.

Method: Electron microscopy
Resolution: 3.09 Å
Organism: Escherichia coli K-12
Chains: 5
Atoms: 11,215
Mol. weight: 167.82 kDa
Ligands: Z41, PLM
Released: 21 Jan 2026

Explore 9RLG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9RLG contains 55 α-helices and 72 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 19 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	5-14	10
α-helix	21-27	7
α-helix	29-55	27
α-helix	56-60	5
β-strand	66-67	2	1
β-strand	70	1	2
β-strand	75	1	3
α-helix	82-84	3
β-strand	90-96	7	1
β-strand	98-104	7	4
β-strand	109-117	9	4
α-helix	126-128	3
β-strand	129-130	2	4
α-helix	134-136	3
β-strand	143-147	5	4
α-helix	148-154	7
β-strand	160-161	2	4
β-strand	163-165	3	4
β-strand	181-182	2	4
α-helix	184	1
β-strand	185-190	6	4
α-helix	195-198	4
β-strand	200-204	5	4
α-helix	205-211	7
α-helix	214-215	2
β-strand	219	1	3
β-strand	221-226	6	1
α-helix	235-237	3
α-helix	240-241	2
β-strand	244	1	2
β-strand	247-248	2	1
α-helix	250-292	43
α-helix	294-303	10
α-helix	307-347	41
α-helix	362-389	28
α-helix	392-395	4

Chain D: 8 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	10
β-strand	13-15	3	11
β-strand	22-25	4	11
β-strand	31-33	3	10
β-strand	37-41	5	12
α-helix	50-55	6
β-strand	65-68	4	10
β-strand	72	1	10
α-helix	73-75	3
α-helix	78-88	11
β-strand	90-93	4	12
α-helix	104-114	11
α-helix	119-133	15
α-helix	149-159	11
β-strand	166-170	5	12
α-helix	178-194	17
β-strand	198-202	5	12
α-helix	208-210	3
β-strand	214-218	5	12
β-strand	223-224	2	12

Chain E: 17 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	5-15	11
α-helix	22-58	37
β-strand	66-70	5	5
α-helix	78-86	9
β-strand	91	1	6
β-strand	96-97	2	7
β-strand	98-99	2	8
β-strand	102-105	4	9
β-strand	111-113	3	9
β-strand	114-118	5	8
α-helix	120-126	7
α-helix	129-132	4
β-strand	133	1	8
β-strand	147-151	5	8
α-helix	152-158	7
β-strand	166-170	5	9
α-helix	181-182	2
β-strand	183-187	5	9
β-strand	188-193	6	8
α-helix	198-202	5
β-strand	203-207	5	8
α-helix	208-214	7
β-strand	223-224	2	7
β-strand	225	1	5
β-strand	228	1	6
α-helix	231-233	3
α-helix	234-244	11
β-strand	249-253	5	5
α-helix	255-297	43
α-helix	299-308	10
α-helix	312-343	32
α-helix	345-356	12
α-helix	377-403	27
α-helix	407-411	5

Chain F: 8 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	6-9	4	13
β-strand	13-15	3	14
β-strand	22-25	4	14
β-strand	31-32	2	13
β-strand	37-41	5	15
α-helix	50-55	6
β-strand	65-68	4	13
β-strand	72	1	13
α-helix	73-75	3
α-helix	78-88	11
β-strand	90-92	3	15
α-helix	104-114	11
α-helix	119-133	15
α-helix	150-159	10
β-strand	166-170	5	15
α-helix	178-195	18
β-strand	198-202	5	15
α-helix	208-210	3
β-strand	214-218	5	15
β-strand	223-224	2	15

Chain L: 3 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	12-14	3	5
α-helix	16-27	12
β-strand	30-40	11	16
β-strand	46-55	10	16
β-strand	59-65	7	16
β-strand	71-78	8	16
β-strand	81-85	5	16
β-strand	91-94	4	16
α-helix	97-105	9
α-helix	111-117	7
β-strand	128-130	3	16
β-strand	136-143	8	16
β-strand	146-152	7	16
β-strand	155-156	2	17
β-strand	163-164	2	17
β-strand	166-170	5	16
β-strand	175-185	11	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lipoprotein-releasing system transmembrane protein LolC	C	protein	399	Escherichia coli K-12	P0ADC3 (AlphaFold model)
Lipoprotein-releasing system transmembrane protein LolE	E	protein	414	Escherichia coli K-12	P75958 (AlphaFold model)
Lipoprotein-releasing system ATP-binding protein LolD	D, F	protein	241	Escherichia coli K-12	P75957 (AlphaFold model)
Outer-membrane lipoprotein LolB	L	protein	227	Escherichia coli K-12	P61320 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>9RLG_1 Lipoprotein-releasing system transmembrane protein LolC (chains C)
MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTVLSVMNGFERELQNNIL
GLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVVLQSARSVAVGVMLGIDPA
QKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGDQIRVMVPSASQFTPMGRIPS
QRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGNITGWRLWLDEPLKVDSLSQQKL
PEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVAVAAFNIITSLGLMVMEKQGEVAIL
QTQGLTPRQIMMVFMVQGASAGIIGAILGAALGALLASQLNNLMPIIGVLLDGAALPVAI
EPLQVIVIALVAMAIALLSTLYPSWRAAATQPAEALRYE

Sequence of entity 2 (E), FASTA

>9RLG_2 Lipoprotein-releasing system transmembrane protein LolE (chains E)
MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVGLSAMNGFERELNNRIL
AVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTGLVESGANLRAIQVKGVNP
QQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKVKQGDWVSIMIPNSNPEHKLM
QPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSSVSGIALKMTDVFNANKLVRD
AGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVLVIGVACFNIVSTLVMAVKDKSG
DIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCGVIIGVVVSLQLTPIIEWIEKLIGHQF
LSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLASWYPARRASNIDPARVLSGQ

Sequence of entity 3 (D, F), FASTA

>9RLG_3 Lipoprotein-releasing system ATP-binding protein LolD (chains D, F)
MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSSGSGKSTLLHLLGGLDT
PTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDFTALENVAMPLLIGKKKPA
EINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARALVNNPRLVLADEPTGNLDARN
ADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEMRDGRLTAELSLMGAEGSHHHHH
H

Sequence of entity 4 (L), FASTA

>9RLG_4 Outer-membrane lipoprotein LolB (chains L)
CSVTTPKGPGKSPDSPQWRQHQQDVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRY
RLLLTNPLGSTELELNAQPGNVQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILG
LPGDATDYKLDDQYRLSEITYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKM
DNWIVKGSGENLYFQSGSAWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Ligands and cofactors

ID	Name	Formula	Copies
Z41	(2S)-3-hydroxypropane-1,2-diyl dihexadecanoate	C35 H68 O5	1
PLM	Palmitic acid	C16 H32 O2	1

Primary citation

Liganded LolCDE structures reveal a common substrate-LolE interaction guiding bacterial lipoprotein transport. Szewczyk, P., Greene, N.P., Symmons, M.F. et al. Proc Natl Acad Sci U S A (2026) 123:e2520579123-e2520579123. DOI 10.1073/pnas.2520579123 · PubMed

Other PDB entries of the same protein (UniProt P0ADC3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5NAA 1.88 Å, Lipoprotein-releasing system transmembrane protein LolC
6F3Z 2.0 Å, Complex of E. coli LolA and periplasmic domain of LolC
6F49 2.02 Å, Periplasmic domain of LolC lacking the Hook.
9RLC 2.8 Å, LolCDE complex with Lpp lipoprotein
9XRO 2.88 Å, LolCDE in complex with SMT-738_3
9RLJ 2.9 Å, LolCDE with bound ATPgammaS
9RLD 3.0 Å, LolCDE complex with Lpp lipoprotein
9RLF 3.17 Å, LolCDE complex with Pal lipoprotein
9RLE 3.18 Å, LolCDE complex with Pal lipoprotein
9RLH 3.19 Å, LolCDE complex with del 9-15 LolB lipoprotein
7ARJ 3.2 Å, LolCDE in complex with lipoprotein and AMPPNP complex undimerized form
7ARL 3.2 Å, LolCDE in complex with lipoprotein and ADP

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