9T88: ESG-2-37-bound D3 dopamine receptor with mini-Go

Cryo-EM structure of ESG-2-37-bound D3 dopamine receptor with mini-Go. Determined by electron microscopy at 2.74 Å resolution. Released 9 Sept 2026.

Method: Electron microscopy
Resolution: 2.74 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,732
Mol. weight: 163.44 kDa
Ligands: A1JUH
Released: 9 Sept 2026

Explore 9T88 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9T88 contains 36 α-helices and 60 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	5-29	25
β-strand	31-38	8	8
α-helix	44-46	3
β-strand	52-53	2	8
β-strand	68-73	6	8
β-strand	78-83	6	8
α-helix	91-93	3
α-helix	95-98	4
β-strand	103-109	7	8
α-helix	116-128	13
α-helix	130-132	3
β-strand	136-142	7	8
α-helix	144-150	7
α-helix	156-158	3
α-helix	169-182	14
β-strand	191-195	5	8
α-helix	203-223	21

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	29-32	4
β-strand	45-50	6	1
β-strand	57-62	6	2
β-strand	68-73	6	2
β-strand	77-82	6	2
β-strand	87-93	7	2
β-strand	102-104	3	3
β-strand	110-113	4	3
β-strand	115	1	4
β-strand	120-124	5	3
β-strand	133-138	6	3
β-strand	145-152	8	4
β-strand	155-160	6	4
β-strand	164-169	6	4
β-strand	174-180	7	4
β-strand	186-191	6	5
β-strand	197-202	6	5
β-strand	206-211	6	5
β-strand	216-222	7	5
β-strand	228-233	6	6
β-strand	239-244	6	6
β-strand	249-253	5	6
β-strand	258-263	6	6
β-strand	272-277	6	7
β-strand	283-288	6	7
β-strand	293-297	5	7
β-strand	303-307	5	7
β-strand	314-319	6	1
β-strand	326-330	5	1
β-strand	335-339	5	1

Chain C: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3
α-helix	56-58	3

Chain R: 13 helices, 0 β-strands

Element	Residues	Length
α-helix	30-56	27
α-helix	63-81	19
α-helix	83-92	10
α-helix	100-133	34
α-helix	135-138	4
α-helix	144-169	26
α-helix	186-193	8
α-helix	194-198	5
α-helix	199-225	27
α-helix	322-354	33
α-helix	359-361	3
α-helix	362-384	23
α-helix	388-398	11

Chain S: 6 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	5-8	4	9
β-strand	11-12	2	10
β-strand	19-25	7	9
α-helix	30-32	3
β-strand	34-40	7	10
β-strand	46-52	7	10
α-helix	54-56	3
β-strand	59-61	3	10
β-strand	69-74	6	9
β-strand	79-84	6	9
β-strand	93-100	8	10
β-strand	112	1	10
β-strand	116-119	4	10
α-helix	138-140	3
β-strand	141-142	2	11
β-strand	147-149	3	12
β-strand	156-162	7	11
β-strand	167	1	13
β-strand	173	1	13
β-strand	175-180	6	12
β-strand	187-191	5	12
β-strand	195-196	2	12
α-helix	197	1
β-strand	204-209	6	11
β-strand	212-217	6	11
β-strand	226-232	7	12
α-helix	239	1
β-strand	240	1	12
α-helix	241	1
β-strand	244-247	4	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	366	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
D(3) dopamine receptor,Lgbit	R	protein	573	Homo sapiens	P35462 (AlphaFold model)
Engineered miniGo,Guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding…	A	protein	226	Homo sapiens	P09471 (AlphaFold model)
scFv16	S	protein	256	Mus musculus

Sequence of entity 1 (B), FASTA

>9T88_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWR
LFKKIS

Sequence of entity 2 (C), FASTA

>9T88_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (R), FASTA

>9T88_3 D(3) dopamine receptor,Lgbit (chains R)
MASLSQLSGHLNYTCGAENSTGASQARPHAYYALSYCALILAIVFGNGLVCMAVLKERAL
QTTTNYLVVSLAVADLLVATLVMPWVVYLEVTGGVWNFSRICCDVFVTLDVMMCTASILN
LCAISIDRYTAVVMPVHYQHGTGQSSCRRVALMITAVWVLAFAVSCPLLFGFNTTGDPTV
CSISNPDFVIYSSVVSFYLPFGVTVLVYARIYVVLKQRRRKRILTRQNSQCNSVRPGFPQ
QTLSPDPAHLELKRYYSICQDTALGGPGFQERGGELKREEKTRNSLSPTIAPKLSLEVRK
LSNGRLSTSLKLGPLQPRGVPLREKKATQMVAIVLGAFIVCWLPFFLTHVLNTHCQTCHV
SPELYSATTWLGYVNSALNPVIYTTFNIEFRKAFLKILSCGSSGGGGSGGGGSSGVFTLE
DFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEG
LSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKK
ITVTGTLWNGNKIIDERLITPDGSMLFRVTINS

Sequence of entity 4 (A), FASTA

>9T88_4 Engineered miniGo,Guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding protein G(o) subunit alpha (chains A)
MTVSAEDKAAAERSKMIEKNLKEDGISAAKDVKLLLLGADNSGKSTIVKQMKIIHGGSGG
SGGTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVDLSDYNRMHESL
MLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDAAAYIQAQF
ESKNRSPNKEIYCHMTCATDTNNAQVIFDAVTDIIIANNLRGCGLY

Sequence of entity 5 (S), FASTA

>9T88_5 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ

Ligands and cofactors

ID	Name	Formula	Copies
A1JUH	~{N}-[4-[2-[4-(2-fluoranyl-3-methoxy-phenyl)piperazin-1-yl]ethyl]cyclohexyl]-3-…	C23 H36 F N3 O3	1

Primary citation

The structure of the dopamine D 3 receptor bound to cariprazine reveals principles for partial agonists with designed pharmacology. Hadas Yardeni, E., Kiss, D.J., Sanchez, J. et al. Sci Adv (2026) 12. DOI 10.1126/sciadv.aef7411 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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