9VEI: Human KCNQ1-KCNE1-CaM complex with PIP2

structure of human KCNQ1-KCNE1-CaM complex with PIP2. Determined by electron microscopy at 3.9 Å resolution. Released 20 Aug 2025.

Method: Electron microscopy
Resolution: 3.9 Å
Organism: Homo sapiens
Chains: 12
Atoms: 15,940
Mol. weight: 383.07 kDa
Ligands: CA, PT5
Released: 20 Aug 2025

Explore 9VEI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9VEI contains 120 α-helices and 8 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, D, G and J: 19 helices, 0 β-strands

Element	Residues	Length
α-helix	105-114	10
α-helix	119-145	27
α-helix	149-151	3
α-helix	155-176	22
α-helix	177-179	3
α-helix	182-184	3
α-helix	186-193	8
α-helix	197-216	20
α-helix	226-237	12
α-helix	238-241	4
α-helix	246-257	12
α-helix	259-284	26
α-helix	299-310	12
α-helix	323-336	14
α-helix	338-340	3
α-helix	342-382	41
α-helix	507-533	27
α-helix	552-560	9
α-helix	588-619	32

Chains B, E, H and K: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	10-18	9
α-helix	30-40	11
α-helix	46-56	11
α-helix	69-73	5
α-helix	80-91	12
β-strand	101-102	2	1
α-helix	103-112	10
α-helix	119-129	11
β-strand	136-137	2	1
α-helix	139-146	8

Chains C, F, I and L: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	42-67	26
α-helix	69-70	2
α-helix	76-79	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Potassium voltage-gated channel subfamily KQT member 1	A, D, G, J	protein	546	Homo sapiens	P51787 (AlphaFold model)
Calmodulin-1	B, E, H, K	protein	149	Homo sapiens	P0DP23 (AlphaFold model)
Potassium voltage-gated channel subfamily E member 1	C, F, I, L	protein	129	Homo sapiens	P15382 (AlphaFold model)

Sequence of entity 1 (A, D, G, J), FASTA

>9VEI_1 Potassium voltage-gated channel subfamily KQT member 1 (chains A, D, G, J)
MASDLGPRPPVSLDPRVSIYSTRRPVLARTHVQGRVYNFLERPTGWKCFVYHFAVFLIVL
VCLIFSVLSTCEQYAALATGTLFWMEIVLVVFFGTEYVVRLWSAGCRSKYVGLWGRLRFA
RKPISIIDLIVVVASMVVLCVGSKGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSV
VFIHRQELITTLYIGFLGLIFSSYFVYLAEKDAVNESGRVEFGSYADALWWGVVTVTTIG
YGDKVPQTWVGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAASL
IQTAWRCYAAENPDSSTWKIYIRKAPRSHTLLSPSPKPKKSVVVKKKKFKLDKDNGVTPG
EKMLTVPHITCDPPEERRLDHFSVDGYDSSVRKSPTLLEVSMPHFMRTNSFAEDLDLEGE
TLLTPITHISQLREHHRATIKVIRRMQYFVAKKKFQQARKPYDVRDVIEQYSQGHLNLMV
RIKELQRRLDQSIGKPSLFISVSEKSKDRGSNTIGARLNRVEDKVTQLDQRLALITDMLH
QLLSLH

Sequence of entity 2 (B, E, H, K), FASTA

>9VEI_2 Calmodulin-1 (chains B, E, H, K)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Sequence of entity 3 (C, F, I, L), FASTA

>9VEI_3 Potassium voltage-gated channel subfamily E member 1 (chains C, F, I, L)
MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSSDGCLEALYVLMVLGFFGFFTLG
IMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYRSCYVVENHLAIEQPNT
HLPETKPSP

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	8
PT5	[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4…	C47 H85 O19 P3	8

Primary citation

Mechanisms of KCNQ1 gating modulation by KCNE1/3 for cell-specific function. Cui, C., Zhao, L., Kermani, A.A. et al. Cell Res (2025) 35:876-886. DOI 10.1038/s41422-025-01152-1 · PubMed

Other PDB entries of the same protein (UniProt P51787 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3HFE 1.7 Å, A trimeric form of the Kv7.1 A domain Tail
3BJ4 2.0 Å, The KCNQ1 (Kv7.1) C-terminus, a multi-tiered scaffold for subunit assembly and protein…
7VVH 2.3 Å, Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease…
3HFC 2.45 Å, A trimeric form of the Kv7.1 A domain Tail, L602M/L606M mutant Semet
7XNN 2.5 Å, human KCNQ1-CaM-ML277-PIP2 complex in state B
9UJ4 2.59 Å, Structure of human KCNQ1-CaM complex
7XNK 2.6 Å, human KCNQ1-CaM in complex with ML277
7VUO 2.68 Å, Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease…
9VEC 2.7 Å, structure of human KCNQ1-KCNE1-CaM complex
4V0C 2.86 Å, Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
8SIK 2.9 Å, KCNQ1 with voltage sensor in the up conformation
9U7F 2.9 Å, structure of human KCNQ1-KCNE1-CaM complex

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