9W9I: Kinesin-2 tail domain

Cryo-EM structure of the kinesin-2 tail domain in complex with KAP3 and APC. Determined by electron microscopy at 2.9 Å resolution. Released 1 Oct 2025.

Method
Electron microscopy
Resolution
2.9 Å
Organism
Mus musculus
Chains
4
Atoms
7,711
Mol. weight
202.17 kDa
Released
1 Oct 2025

Explore 9W9I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9W9I contains 70 α-helices and 6 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 2 β-strands

ElementResiduesLengthSheet
α-helix570-59728
α-helix600-6089
β-strand610-61341
β-strand618-62141
Chain B: 6 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix563-59028
α-helix593-60210
β-strand603-60642
β-strand611-61442
α-helix624-6296
α-helix641-6499
α-helix653-6553
α-helix663-6653
Chain C: 42 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix140-1434
α-helix149-16113
α-helix163-1653
α-helix166-1716
α-helix176-1838
α-helix192-1976
α-helix201-2055
α-helix210-2178
α-helix219-24426
α-helix245-2473
β-strand25413
β-strand25713
α-helix260-28829
α-helix293-3019
α-helix304-3118
α-helix317-33115
α-helix334-3418
α-helix346-3505
α-helix358-37114
α-helix375-38410
α-helix386-3938
α-helix400-41112
α-helix414-4207
α-helix428-4358
α-helix443-45210
α-helix456-4627
α-helix467-47711
α-helix481-49010
α-helix496-5005
α-helix501-5044
α-helix505-5117
α-helix521-5299
α-helix539-5446
α-helix548-5547
α-helix562-57413
α-helix580-5878
α-helix590-60011
α-helix605-61915
α-helix624-6263
α-helix627-6315
α-helix634-6407
α-helix647-66317
α-helix665-67915
α-helix681-6877
Chain D: 20 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix470-48415
α-helix490-50718
α-helix511-5188
α-helix521-5299
α-helix530-5323
α-helix536-55015
α-helix555-5639
α-helix566-57611
α-helix580-59314
α-helix598-6069
α-helix611-6144
α-helix615-6173
α-helix628-64417
α-helix648-6558
α-helix659-6657
α-helix666-6683
α-helix672-68514
α-helix690-6989
α-helix701-7077
α-helix714-72815

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Kinesin-like protein KIF3AAprotein178Mus musculusP28741 (AlphaFold model)
Kinesin-like protein KIF3B, N-terminally processedBprotein220Mus musculusQ61771 (AlphaFold model)
Kinesin-associated protein 3Cprotein693Mus musculusP70188 (AlphaFold model)
Adenomatous polyposis coli proteinDprotein680Mus musculusQ61315
Sequence of entity 1 (A), FASTA
>9W9I_1 Kinesin-like protein KIF3A (chains A)
MGSSHHHHHHSQSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQL
SRELRLQMLIIDNFIPQDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKER
DPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKMGRRKRSAKPETVIDSLLQ
Sequence of entity 2 (B), FASTA
>9W9I_2 Kinesin-like protein KIF3B, N-terminally processed (chains B)
MSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNELTRELKLKHLII
ENFIPLEEKNKIMNRSFFDDEEDHWKLHPITRLENQQMMKRPVSAVGYKRPLSQHARMSM
MIRPEPRYRAENIMLLELDMPSRTTRDYEGPAISPKVQAALDAALQDEDEIQVDASSFES
TASRKPKARPKSGRKSGSSSSSSGNPASQFYPQSRGLVPK
Sequence of entity 3 (C), FASTA
>9W9I_3 Kinesin-associated protein 3 (chains C)
MQGEDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKS
LNANTDITSLARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPP
FEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALA
RVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHYKIGALCMNIIDHELKRHELWQEE
LSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMR
NKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDL
LNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMF
AYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKD
PLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISSDEEEEFVIECLGTLANLTIPDLDWEL
VLKEYKLVPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNA
QQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDII
AEYDEEWAKKIQSEKFRWHNSQWLEMVESRQLD
Sequence of entity 4 (D), FASTA
>9W9I_4 Adenomatous polyposis coli protein (chains D)
MHHHHHHSQDSCISMRQSGCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASAALHNIIH
SQPDDKRGRREIRVLHLLEQIRAYCETCWEWQEAHEQGMDQDKNPMPAPVEHQICPAVCV
LMKLSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTNDHYSVTLRRYAGMALTNLTFGD
VANKATLCSMKGCMRALVAQLKSESEDLQQVIASVLRNLSWRADVNSKKTLREVGSVKAL
MECALEVKKESTLKSVLSALWNLSAHCTENKADICAVDGALAFLVGTLTYRSQTNTLAII
ESGGGILRNVSSLIATNEDHRQILRENNCLQTLLQHLKSHSLTIVSNACGTLWNLSARNP
KDQEALWDMGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPAKYKDANIMSPGSSLPSL
HVRKQKALEAELDAQHLSETFDNIDNLSPKASHRSKQRHKQNLYGDYAFDANRHDDSRSD
NFNTGNMTVLSPYLNTTVLPSSSSSRGSLDSSRSEKDRSLERERGIGLSAYHPTTENAGT
SSKRGLQITTTAAQIAKVMEEVSAIHTSQDDRSSASTTEFHCVADDRSAARRSSASHTHS
NTYNFTKSENSNRTCSMPYAKVEYKRSSNDSLNSVTSSDGYGKRGQMKPSVESYSEDDES
KFCSYGQYPADLAHKIHSAN

Primary citation

The hook-like adaptor and cargo-binding (HAC) domain in the kinesin-2 tail enables adaptor assembly and cargo recognition. Jiang, X., Danev, R., Ichinose, S. et al. Sci Adv (2025) 11:eady5861-eady5861. DOI 10.1126/sciadv.ady5861 · PubMed

Other PDB entries of the same protein (UniProt P28741 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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