9WNF: 5HT2AR-miniGq heterotrimer

5HT2AR-miniGq heterotrimer in complex with a selective agonist IHCH-2330. Determined by electron microscopy at 3.13 Å resolution. Released 9 Sept 2026.

Method: Electron microscopy
Resolution: 3.13 Å
Organism: Homo sapiens
Chains: 4
Atoms: 7,232
Mol. weight: 140.66 kDa
Ligands: A1EXY
Released: 9 Sept 2026

Explore 9WNF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9WNF contains 33 α-helices and 62 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	81-100	20
α-helix	108-136	29
α-helix	145-178	34
α-helix	180-186	7
α-helix	189-207	19
α-helix	209-213	5
α-helix	218-220	3
β-strand	222-223	2	1
β-strand	226-227	2	1
α-helix	232-239	8
α-helix	240-244	5
α-helix	245-260	16
α-helix	316-347	32
α-helix	356-381	26
α-helix	385-391	7

Chain B: 13 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	10-23	14
α-helix	30-43	14
α-helix	45-47	3
α-helix	87-110	24
β-strand	114-117	4	2
β-strand	150-156	7	2
β-strand	159-165	7	2
α-helix	176-180	5
β-strand	185-186	2	2
β-strand	189-191	3	3
α-helix	195-197	3
α-helix	199-209	11
α-helix	212-214	3
β-strand	218-219	2	2
β-strand	222-224	3	3
α-helix	226-235	10
α-helix	240-243	4
α-helix	245-249	5
α-helix	264-282	19
β-strand	291	1	2
β-strand	294-295	2	3
α-helix	303-322	20

Chain C: 5 helices, 33 β-strands

Element	Residues	Length	Sheet
α-helix	7-24	18
α-helix	30-34	5
α-helix	38-41	4
β-strand	43	1	4
β-strand	47-50	4	5
β-strand	52	1	6
β-strand	54	1	6
β-strand	58-63	6	7
β-strand	69-74	6	7
β-strand	78-83	6	7
β-strand	88-94	7	7
α-helix	95	1
β-strand	103-105	3	8
β-strand	111-114	4	8
β-strand	120-125	6	8
β-strand	134-140	7	8
β-strand	146-153	8	9
β-strand	156-161	6	9
β-strand	165-170	6	9
β-strand	175-181	7	9
β-strand	187	1	10
β-strand	191-192	2	10
β-strand	198-203	6	10
β-strand	207-212	6	10
β-strand	218-223	6	10
β-strand	229-234	6	11
β-strand	240-245	6	11
β-strand	249-254	6	11
β-strand	259-265	7	11
α-helix	271-272	2
β-strand	273-278	6	12
β-strand	284-289	6	12
β-strand	294-298	5	12
β-strand	304	1	12
β-strand	306	1	4
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	336-339	4	5

Chain D: 2 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	13
α-helix	12-13	2
β-strand	18-25	8	13
β-strand	33-39	7	14
β-strand	45-51	7	14
α-helix	53-55	3
β-strand	58-60	3	14
β-strand	68-73	6	13
β-strand	78-83	6	13
β-strand	93-99	7	14
β-strand	110-111	2	14
β-strand	155-160	6	15
β-strand	175-179	5	14
β-strand	186-190	5	14
β-strand	194-195	2	14
β-strand	205-208	4	15
β-strand	211-216	6	15
β-strand	226-230	5	14
β-strand	239	1	14
β-strand	243-244	2	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
5-hydroxytryptamine receptor 2A	A	protein	346	Homo sapiens	P28223 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,Guanine nucleotide-binding…	B	protein	326	Homo sapiens	P59768 (AlphaFold model), Q5JWF2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	340	Homo sapiens	P62873 (AlphaFold model)
single-chain variable fragment 16 (scFv16)	D	protein	258	Homo sapiens

Sequence of entity 1 (A), FASTA

>9WNF_1 5-hydroxytryptamine receptor 2A (chains A)
GPGSGSGLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAI
ADMLLGFLVMPVSMLTILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQ
NPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIG
SFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLASFSFLPQSSLSSEKLFQRS
IHREPGSYTGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNEDVI
GALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKK

Sequence of entity 2 (B), FASTA

>9WNF_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (chains B)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSAMGCTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLG
ADNSGKSTIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCF
NDVTAIIFVVDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKI
EDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVD
TENARRIFAAVKDTILQLNLREYNLV

Sequence of entity 3 (C), FASTA

>9WNF_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
GSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (D), FASTA

>9WNF_4 single-chain variable fragment 16 (scFv16) (chains D)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAENLYFQG

Ligands and cofactors

ID	Name	Formula	Copies
A1EXY	3-[2-[2-[2,5-dimethoxy-4-(trifluoromethyl)phenyl]ethyl-methyl-amino]ethyl]-2-me…	C23 H26 F3 N3 O3	1

Primary citation

Mechanistic Basis for 5-HT 2A R Over 5-HT 2B R Activation. Tang, L., Ji, X., Wang, Y. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-77659-x · PubMed

Other PDB entries of the same protein (UniProt P28223 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7WC8 2.45 Å, Crystal structure of serotonin 2A receptor in complex with lumateperone
7WC9 2.5 Å, Crystal structure of serotonin 2A receptor in complex with non-hallucinogenic…
9AS8 2.54 Å, Global reconstruction of 5-HT2AR bound to psilocin in complex with a mini-Gq protein and…
7WC6 2.6 Å, Crystal structure of serotonin 2A receptor in complex with LSD
7WC7 2.6 Å, Crystal structure of serotonin 2A receptor in complex with lisuride
9LL8 2.62 Å, Psilocin-bound Serotonin 2A (5-HT2A) receptor-Gi complex
8JT8 2.7 Å, Crystal structure of 5-HT2AR in complex with (R)-IHCH-7179
9AS7 2.72 Å, Local refinement of 5-HT2AR bound to psilocin in complex with a mini-Gq and scFv16…
9LL9 2.76 Å, DOI-bound Serotonin 2A (5-HT2A) receptor-Gq complex
8UWL 2.8 Å, 5-HT2AR bound to Lisuride in complex with a mini-Gq protein and an active-state…
9J87 2.84 Å, Structure of Receptor
9LL7 2.84 Å, DOI-bound Serotonin 2A (5-HT2A) receptor-Gi complex

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