9WXM: Full-length GPR15L bound GPR15-Gi complex

Cryo-EM structure of the full-length GPR15L bound GPR15-Gi complex. Determined by electron microscopy at 3.3 Å resolution. Released 19 Nov 2025.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,643
Mol. weight: 165.12 kDa
Released: 19 Nov 2025

Explore 9WXM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9WXM contains 33 α-helices and 73 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	1
α-helix	47-52	6
β-strand	185-191	7	1
β-strand	194-200	7	1
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	1
β-strand	233	1	2
β-strand	241	1	2
α-helix	242-253	12
β-strand	256	1	3
β-strand	259	1	3
β-strand	263-269	7	1
α-helix	271-278	8
α-helix	283-285	3
α-helix	297-308	12
β-strand	319-323	5	1
α-helix	328-351	24

Chain B: 3 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	4-23	20
α-helix	30-33	4
α-helix	38-42	5
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	88-94	7	5
β-strand	103-105	3	6
β-strand	111-114	4	6
β-strand	120	1	7
β-strand	123-125	3	6
β-strand	134-137	4	6
β-strand	140	1	7
β-strand	146-151	6	8
β-strand	156-161	6	8
β-strand	165-170	6	8
β-strand	176-181	6	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	207-212	6	9
β-strand	218-223	6	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	260-264	5	10
β-strand	273-276	4	11
β-strand	284	1	12
β-strand	287-289	3	11
β-strand	293-297	5	11
β-strand	298	1	12
β-strand	304-309	6	11
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-339	4	4

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	11-24	14
α-helix	30-43	14

Chain D: 3 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	13
β-strand	7	1	14
β-strand	10-12	3	15
β-strand	18	1	16
β-strand	21	1	14
β-strand	24-25	2	13
β-strand	33-35	3	17
β-strand	38-39	2	15
β-strand	45-46	2	15
β-strand	49-51	3	17
α-helix	53-55	3
β-strand	58-60	3	17
α-helix	62-64	3
β-strand	68	1	16
β-strand	71-73	3	14
β-strand	78-80	3	14
β-strand	83	1	16
β-strand	92-94	3	15
β-strand	97-99	3	17
β-strand	110-111	2	17
β-strand	115-119	5	15
β-strand	128-130	3	18
β-strand	134-136	3	19
β-strand	142-149	8	18
β-strand	154	1	20
β-strand	160	1	20
β-strand	162-167	6	21
β-strand	173-178	6	21
β-strand	182-183	2	21
α-helix	184	1
β-strand	194-195	2	18
β-strand	199-205	7	18
β-strand	214-219	6	21
β-strand	232-234	3	19

Chain L: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	45-47	3

Chain R: 15 helices, 0 β-strands

Element	Residues	Length
α-helix	35-59	25
α-helix	64-66	3
α-helix	67-80	14
α-helix	86-88	3
α-helix	104-135	32
α-helix	141-144	4
α-helix	147-164	18
α-helix	166-171	6
α-helix	190-200	11
α-helix	201-205	5
α-helix	206-227	22
α-helix	233-236	4
α-helix	237-270	34
α-helix	279-304	26
α-helix	309-317	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protein GPR15LG	L	protein	58	Homo sapiens	Q6UWK7 (AlphaFold model)
G-protein coupled receptor 15	R	protein	379	Homo sapiens	P49685 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-3	A	protein	354	Homo sapiens	P08754 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768
scFv16	D	protein	267	Mus musculus

Sequence of entity 1 (L), FASTA

>9WXM_1 Protein GPR15LG (chains L)
GKRRPAKAWSGRRTRLCCHRVPSPNSTNLKGHHVRLCKPCKLEPEPRLWVVPGALPQV

Sequence of entity 2 (R), FASTA

>9WXM_2 G-protein coupled receptor 15 (chains R)
GAPMDPEETSVYLDYYYATSPNSDIRETHSHVPYTSVFLPVFYTAVFLTGVLGNLVLMGA
LHFKPGSRRLIDIFIINLAASDFIFLVTLPLWVDKEASLGLWRTGSFLCKGSSYMISVNM
HCSVLLLTCMSVDRYLAIVWPVVSRKFRRTDCAYVVCASIWFISCLLGLPTLLSRELTLI
DDKPYCAEKKATPIKLIWSLVALIFTFFVPLLSIVTCYCCIARKLCAHYQQSGKHNKKLK
KSIKIIFIVVAAFLVSWLPFNTFKFLAIVSGLRQEHYLPSAILQLGMEVSGPLAFANSCV
NPFIYYIFDSYIRRAIVHCLCPCLKNYDFGSSTEFLEVLFQGPWSHPQFEKGGGSGGGSG
GSAWSHPQFEKDYKDDDDK

Sequence of entity 3 (A), FASTA

>9WXM_3 Guanine nucleotide-binding protein G(i) subunit alpha-3 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKCTIVKQMKIIHEDG
YSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMT
PELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVK
TTGIVETHFTFKDLYFKMFDVTAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAA
AYIQCQFEDLNRRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKECGLY

Sequence of entity 4 (B), FASTA

>9WXM_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (C), FASTA

>9WXM_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (D), FASTA

>9WXM_6 scFv16 (chains D)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAALEVLFQGPHHHHHHHH

Primary citation

Molecular insights into ligand recognition and receptor activation of GPR15. Chen, S., Han, X., Zhang, Y. et al. iScience (2025) 28:113935-113935. DOI 10.1016/j.isci.2025.113935 · PubMed

Other PDB entries of the same protein (UniProt Q6UWK7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8ZQE 2.9 Å, Cryo-EM structure of the GPR15L(C11)-bound GPR15 complex

Browse structure collections

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