Muscarinic acetylcholine receptor M2 (CHRM2) is a 466-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P08172.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 72.1 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 51% |
| 70 to 90 | Confident: backbone generally right | 8% |
| 50 to 70 | Low: treat with caution | 9% |
| Below 50 | Very low: often disordered regions | 32% |
What pLDDT means and how to read it
Muscarinic receptor for acetylcholine, a neurotransmitter found in the brain, neuromuscular junctions and the autonomic ganglia (PubMed:24256733, PubMed:3443095, PubMed:36690613). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:36690613). CHRM2 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G proteins and mediates signaling by inhibiting adenylate cyclase activity (PubMed:36690613)
Interacts (when phosphorylated) with ARRB1 and ARRB2; the interaction is associated with internalization of the receptor and short-term desensitization to the ligand (PubMed:38175886, PubMed:7822302). Interacts with RACK1; the interaction regulates CHRM2 internalization (PubMed:20976005)
Cell membrane, Postsynaptic cell membrane
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5ZKC | X-ray | 2.3 Å | A=10-217, A=377-466 |
| 5YC8 | X-ray | 2.5 Å | A=10-217, A=377-466 |
| 5ZK3 | X-ray | 2.6 Å | A=10-217, A=377-466 |
| 8J8R | EM | 2.9 Å | G/U/V=300-317 |
| 5ZKB | X-ray | 2.95 Å | A=10-217, A=377-466 |
| 3UON | X-ray | 3.0 Å | A=1-217, A=377-466 |
| 5ZK8 | X-ray | 3.0 Å | A=10-217, A=377-466 |
| 8JAF | EM | 3.1 Å | V=307-313 |
| 7T94 | EM | 3.16 Å | A=4-232, A=368-466 |
| 8J97 | EM | 3.2 Å | V=305-313 |
| 7T8X | EM | 3.21 Å | A=4-232, A=368-466 |
| 7T96 | EM | 3.22 Å | A=4-232, A=368-466 |
| 7T90 | EM | 3.32 Å | A=4-232, A=368-466 |
| 4MQS | X-ray | 3.5 Å | A=1-466 |
| 6OIK | EM | 3.6 Å | R=3-466 |
| 4MQT | X-ray | 3.7 Å | A=1-466 |
| 6U1N | EM | 4.0 Å | R=2-466 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.