Histone H1.4 (H1-4) is a 219-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P10412.
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The mean pLDDT of this model is 64.8 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 31% |
| 70 to 90 | Confident: backbone generally right | 2% |
| 50 to 70 | Low: treat with caution | 35% |
| Below 50 | Very low: often disordered regions | 32% |
What pLDDT means and how to read it
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed:35581345, PubMed:40240600). Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers and promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex (PubMed:35581345, PubMed:40240600, PubMed:40516528). Ability to associate with nucleosomes and compact chromatin depends on linker DNA length and trajectory (PubMed:35581345). Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (PubMed:40240600)
Associates with nucleosomes, promoting condensation into higher-order structured chromatin
Nucleus, Chromosome
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3TZD | X-ray | 1.81 Å | T=19-36 |
| 6H8P | X-ray | 1.98 Å | C/D=18-32 |
| 5JJZ | X-ray | 2.0 Å | B=21-32 |
| 7K5Y | EM | 2.76 Å | U=1-219 |
| 8H1T | EM | 3.0 Å | K=1-219 |
| 7K63 | EM | 3.03 Å | U=1-35, U=111-219 |
| 8VG2 | EM | 3.04 Å | U=1-219 |
| 9DDE | EM | 3.2 Å | O=1-219 |
| 7PF5 | EM | 3.8 Å | u=2-219 |
| 7PF3 | EM | 4.0 Å | s=2-219 |
| 7PF6 | EM | 4.0 Å | U=2-219 |
| 7PFX | EM | 4.3 Å | S=2-219 |
| 7PFD | EM | 4.4 Å | U=2-219 |
| 7PFE | EM | 4.4 Å | u=2-219 |
| 7PFV | EM | 4.4 Å | U=2-219 |
| 7PFU | EM | 5.0 Å | S/U=2-219 |
| 7PEX | EM | 5.1 Å | u=2-219 |
| 7PF2 | EM | 5.1 Å | U=2-219 |
| 7PFW | EM | 5.2 Å | u=2-219 |
| 7PFC | EM | 6.4 Å | U=2-219 |
Showing 20 of 26 experimental structures (best resolution first).
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