7PF2: Histone H3.2

Nucleosome stack of the 4x187 nucleosome array containing H1. Determined by electron microscopy at 5.1 Å resolution. Released 3 Aug 2022.

Method: Electron microscopy
Resolution: 5.1 Å
Organisms: Homo sapiens, synthetic construct
Chains: 19
Atoms: 26,610
Mol. weight: 712.14 kDa
Released: 3 Aug 2022

Explore 7PF2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7PF2 contains 76 α-helices and 43 β-strands across 17 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	45-56	12
α-helix	64-78	15
β-strand	83-84	2	11
α-helix	86-113	28
β-strand	118-119	2	12
α-helix	121-131	11

Chain B: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	25-28	4
α-helix	31-40	10
β-strand	45-46	2	12
α-helix	50-75	26
β-strand	80-81	2	11
α-helix	83-92	10
β-strand	96-98	3	13

Chain C: 6 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	11-13	3
α-helix	17-20	4
α-helix	27-35	9
β-strand	42-43	2	14
α-helix	46-72	27
β-strand	77-78	2	15
α-helix	80-88	9
α-helix	91-97	7
β-strand	101-102	2	16

Chains D and R: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	53-54	2	15
α-helix	56-83	28
β-strand	88-89	2	14
α-helix	91-101	11
α-helix	104-122	19

Chain E: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	41-42	2
α-helix	45-56	12
α-helix	64-76	13
β-strand	83-84	2	17
α-helix	86-113	28
β-strand	118-119	2	18
α-helix	121-131	11

Chain F: 3 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	31-41	11
β-strand	45-46	2	18
α-helix	48-75	28
β-strand	80-81	2	17
α-helix	83-93	11
β-strand	97-98	2	16

Chain G: 6 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	12-14	3
α-helix	17-21	5
α-helix	27-35	9
β-strand	42-43	2	19
α-helix	46-72	27
β-strand	77-78	2	20
α-helix	80-88	9
α-helix	91-96	6
β-strand	100-102	3	13

Chain H: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	53-54	2	20
α-helix	56-83	28
β-strand	88-89	2	19
α-helix	91-100	10
α-helix	104-122	19

8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Histone H3.2	A, E, K, O	protein	136	Homo sapiens	Q71DI3 (AlphaFold model)
Histone H4	B, F, L, P	protein	103	Homo sapiens	P62805 (AlphaFold model)
Histone H2A type 1-B/E	C, G, M, Q	protein	147	Homo sapiens	P04908 (AlphaFold model)
Histone H2B type 1-K	D, H, N, R	protein	126	Homo sapiens	O60814 (AlphaFold model)
Histone H1.4	U	protein	218	Homo sapiens	P10412
DNA (541-mer)	I	DNA	748	synthetic construct
DNA (541-mer)	J	DNA	748	synthetic construct

Sequence of entity 1 (A, E, K, O), FASTA

>7PF2_1 Histone H3.2 (chains A, E, K, O)
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLAAIHAKRVTI
MPKDIQLARRIRGERA

Sequence of entity 2 (B, F, L, P), FASTA

>7PF2_2 Histone H4 (chains B, F, L, P)
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Sequence of entity 3 (C, G, M, Q), FASTA

>7PF2_3 Histone H2A type 1-B/E (chains C, G, M, Q)
HHHHHHENLYFQSNAPWMSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSER
VGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTI
AQGGVLPNIQAVLLPKKTESHHKAKGK

Sequence of entity 4 (D, H, N, R), FASTA

>7PF2_4 Histone H2B type 1-K (chains D, H, N, R)
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSAK

Sequence of entity 5 (U), FASTA

>7PF2_5 Histone H1.4 (chains U)
SETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAASKERSGVSLAA
LKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAK
KAGAAKAKKPAGAAKKPKKATGAATPKKSAKKTPKKAKKPAAAAGAKKAKSPKKAKAAKP
KKAPKSPAKAKAVKPKAAKPKTAKPKAAKPKKAAAKKK

Sequence of entity 6 (I), FASTA

>7PF2_6 DNA (541-MER) (chains I)
ATCTCTCGCGCACTGGCCGCCTGGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAG
ACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAGG
GGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCTGTCATGTAAGTATTA
AGGTAACCCGTCTCGCGCACTGGCCGCCTGGAGAATCCCGGTGCCGAGGCCGCTCAATTG
GTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACC
GCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCTGTCATGTA
AGTATTAAGGTAACCCGTCTCGCGCACTGGCCGCCTGGAGAATCCCGGTGCCGAGGCCGC
TCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGT
TTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATACATCCTG
TCATGTAAGTATTAAGGTAACCCGTCTCGCGCACTGGCCGCCTGGAGAATCCCGGTGCCG
AGGCCGCTCAATTGGTCGTAGACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCC
CCCGCGTTTTAACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATA
CATCCTGTCATGTAAGTATTAAGGTGAT

Sequence of entity 7 (J), FASTA

>7PF2_7 DNA (541-MER) (chains J)
ATCACCTTAATACTTACATGACAGGATGTATATATCTGACACGTGCCTGGAGACTAGGGA
GTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTA
GAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTCCAGGCGGCCAGTGCGC
GAGACGGGTTACCTTAATACTTACATGACAGGATGTATATATCTGACACGTGCCTGGAGA
CTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCGTTTAAGC
GGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTCCAGGCGGCC
AGTGCGCGAGACGGGTTACCTTAATACTTACATGACAGGATGTATATATCTGACACGTGC
CTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGCG
TTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGATTCTCCA
GGCGGCCAGTGCGCGAGACGGGTTACCTTAATACTTACATGACAGGATGTATATATCTGA
CACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGT
ACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTGAGCGGCCTCGGCACCGGGA
TTCTCCAGGCGGCCAGTGCGCGAGAGAT

Primary citation

Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory. Dombrowski, M., Engeholm, M., Dienemann, C. et al. Nat Struct Mol Biol (2022) 29:493-501. DOI 10.1038/s41594-022-00768-w · PubMed

Other PDB entries of the same protein (UniProt Q71DI3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2X4W 1.5 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
4MZG 1.7 Å, Crystal structure of human Spindlin1 bound to histone H3K4me3 peptide
2X4Y 1.7 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
2X4X 1.85 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
4OUC 1.9 Å, Structure of human haspin in complex with histone H3 substrate
5VAC 1.95 Å, Crystal Structure of ATXR5 SET domain in complex with K36me3 histone H3 peptide
6ACE 1.98 Å, histone lysine desuccinylase Sirt5 in complex with succinyl peptide H3K122
7UVA 1.98 Å, Crystal structure of KDM2A histone demethylase catalytic domain in complex with an H3C36…
5B0Z 1.99 Å, The crystal structure of the nucleosome containing H3.2, at 1.98 A resolution
3R93 2.06 Å, Crystal structure of the chromo domain of M-phase phosphoprotein 8 bound to H3K9Me3…
4MZF 2.1 Å, Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide
4MZH 2.2 Å, Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2s) peptide

Browse structure collections

About this viewer

MolViewer shows 7PF2 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.