POU domain, class 5, transcription factor 1 (Pou5f1) is a 352-residue protein from Mus musculus. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P20263.
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The mean pLDDT of this model is 65.0 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 31% |
| 70 to 90 | Confident: backbone generally right | 7% |
| 50 to 70 | Low: treat with caution | 22% |
| Below 50 | Very low: often disordered regions | 40% |
What pLDDT means and how to read it
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1690859, PubMed:17525163, PubMed:1967980, PubMed:1972777, PubMed:23376973). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:15863505, PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:18687992, PubMed:1972777, PubMed:19740739, PubMed:23376973, PubMed:29153991, PubMed:32703285)
Interacts with PKM. Interacts with WWP2 (By similarity). Interacts with UBE2I and ZSCAN10 (PubMed:17496161, PubMed:19740739). Interacts with PCGF1 (By similarity). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (PubMed:18662995). Interacts with ZNF322 (PubMed:24550733). Interacts with MAPK8 and MAPK9; the…
Cytoplasm, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 8BX1 | X-ray | 2.5 Å | A=131-282 |
| 3L1P | X-ray | 2.8 Å | A/B=131-282 |
| 8BX2 | X-ray | 3.14 Å | A=131-282 |
| 6HT5 | X-ray | 3.45 Å | E=131-282 |
| 1OCP | NMR | A=217-282 |
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