DDK kinase regulatory subunit DBF4 (DBF4) is a 704-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P32325.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 59.8 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 16% |
| 70 to 90 | Confident: backbone generally right | 24% |
| 50 to 70 | Low: treat with caution | 14% |
| Below 50 | Very low: often disordered regions | 45% |
What pLDDT means and how to read it
Regulatory subunit of the CDC7-DBF4 kinase, also called DBF4-dependent kinase (DDK), which is involved in cell cycle regulation of premitotic and premeiotic chromosome replication and in chromosome segregation. DDK plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. DBF4 recruits the catalytic subunit CDC7 to MCM2 and to origins of replication. DDK also has postreplicative functions in meiosis. DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar…
Heterodimer with CDC7 to form the DBF4-dependent kinase (DDK) complex. Interacts (via PBD-binding motif) with CDC5 (via POLO box domains). Interacts (via N-terminus) with ORC2, ORC3 and RAD53. Binds to ARS1 origin DNA
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3OQ4 | X-ray | 2.4 Å | A/B/C/D/E=120-250 |
| 5T2S | X-ray | 2.4 Å | A/C=105-220 |
| 5T2F | X-ray | 2.66 Å | A/B/C/D=105-220 |
| 3QBZ | X-ray | 2.69 Å | A=66-221 |
| 3OQ0 | X-ray | 2.7 Å | A/B/C/D/E/F/G/H/I/J=120-250 |
| 7V3V | EM | 2.9 Å | I=1-704 |
| 7PT6 | EM | 3.2 Å | 9/I=1-704 |
| 7P5Z | EM | 3.3 Å | G=1-704 |
| 6MF6 | X-ray | 3.4 Å | C/D=76-96 |
| 7PT7 | EM | 3.8 Å | 9=1-704 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.