7P5Z: DNA replication licensing factor MCM2

Name: PDB 7P5Z: Structure of a DNA-loaded MCM double hexamer engaged with the Dbf4-dependent kinase
Published: 2022-02-02
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

Structure of a DNA-loaded MCM double hexamer engaged with the Dbf4-dependent kinase. Determined by electron microscopy at 3.3 Å resolution. Released 2 Feb 2022.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae S288C
Chains: 16
Atoms: 67,401
Mol. weight: 1401.82 kDa
Ligands: ADP, ZN, MG, ATP
Released: 2 Feb 2022

Explore 7P5Z in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7P5Z contains 399 α-helices and 357 β-strands across 14 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 1: 16 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	17-25	9
α-helix	27-32	6
β-strand	35-41	7	101
β-strand	45-50	6	101
β-strand	73-78	6	101
α-helix	84-95	12
β-strand	108-111	4	101
β-strand	116-121	6	101
α-helix	137-157	21
β-strand	159-160	2	102
α-helix	166-168	3
β-strand	169-172	4	103
β-strand	177-180	4	103
β-strand	187-188	2	102
α-helix	249-251	3
α-helix	255-262	8
α-helix	269	1
α-helix	270-274	5
α-helix	287-291	5
α-helix	300-312	13
α-helix	325-335	11
α-helix	338-347	10
β-strand	354	1	104
α-helix	362-364	3
α-helix	443-447	5
α-helix	460-465	6

Chain 2: 29 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	194-198	5
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-245	5	1
α-helix	246-262	17
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-298	5	1
β-strand	304	1	2
α-helix	311-313	3
β-strand	316-327	12	2
β-strand	331-337	7	2
β-strand	339-341	3	3
β-strand	347	1	3
α-helix	348-350	3
β-strand	351-352	2	2
β-strand	373-375	3	3
β-strand	381-391	11	2
α-helix	394-396	3
α-helix	398	1
β-strand	405-411	7	2
α-helix	412-414	3
β-strand	423-432	10	2
β-strand	446	1	4
β-strand	449-458	10	2
α-helix	478-489	12
α-helix	493-498	6
α-helix	509-520	12
β-strand	525-526	2	5
β-strand	532-533	2	5
β-strand	539-543	5	6
α-helix	549-559	11
β-strand	563-567	5	6
β-strand	579-581	3	7
β-strand	590-592	3	7
α-helix	595-598	4
β-strand	602-607	6	6
α-helix	614-626	13
β-strand	628-633	6	8
β-strand	636-641	6	8
β-strand	644-650	7	6
α-helix	653-655	3
α-helix	663-665	3
α-helix	671-674	4
β-strand	679-683	5	6
α-helix	689-706	18
α-helix	757-759	3
α-helix	760-773	14
β-strand	777	1	9
α-helix	780-782	3
α-helix	783-800	18
α-helix	804-805	2
α-helix	807-823	17
β-strand	828	1	9
α-helix	831-847	17
α-helix	851-860	10

Chain 3: 29 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	37-51	15
β-strand	95-99	5	10
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
β-strand	154-158	5	10
α-helix	162-164	3
β-strand	165-166	2	11
α-helix	168-170	3
α-helix	173-175	3
β-strand	179-189	11	11
β-strand	195-203	9	12
β-strand	208-213	6	12
α-helix	217-219	3
α-helix	229-232	4
β-strand	234	1	13
β-strand	240	1	13
β-strand	241-243	3	12
β-strand	249-251	3	12
β-strand	253-259	7	11
α-helix	266	1
β-strand	273-279	7	11
α-helix	280-282	3
β-strand	291-301	11	11
β-strand	318-322	5	11
β-strand	325-328	4	11
α-helix	337-338	2
α-helix	341-343	3
α-helix	344-354	11
α-helix	359-366	8
α-helix	375-386	12
β-strand	391-392	2	14
β-strand	398-399	2	14
β-strand	404-409	6	15
α-helix	415-425	11
β-strand	429-433	5	15
α-helix	440-443	4
β-strand	447-449	3	16
β-strand	455-457	3	16
α-helix	461-464	4
β-strand	469-473	5	15
α-helix	475-477	3
α-helix	480-491	12
β-strand	494-499	6	17
β-strand	502-507	6	17
β-strand	511-516	6	15
α-helix	529-533	5
α-helix	537-542	6
β-strand	545-549	5	15
α-helix	555-568	14
α-helix	653-666	14
β-strand	670	1	18
α-helix	673-687	15
α-helix	699-716	18
β-strand	720	1	18
α-helix	723-737	15

Chain 4: 35 helices, 36 β-strands

Element	Residues	Length	Sheet
α-helix	162-166	5
β-strand	179	1	19
β-strand	186	1	19
α-helix	187-199	13
α-helix	225-236	12
β-strand	240-243	4	20
α-helix	247-249	3
α-helix	253-255	3
α-helix	257-263	7
α-helix	265-286	22
α-helix	294-298	5
β-strand	302-305	4	20
α-helix	308-310	3
α-helix	314-316	3
α-helix	319-321	3
β-strand	325-335	11	21
α-helix	336-338	3
β-strand	339-349	11	21
β-strand	355-359	5	21
β-strand	361	1	22
β-strand	364-367	4	22
β-strand	370	1	23
β-strand	379	1	23
β-strand	382-384	3	21
α-helix	386-388	3
β-strand	390-401	12	21
β-strand	414-420	7	21
α-helix	421-423	3
β-strand	432-439	8	21
β-strand	442	1	24
β-strand	445	1	25
β-strand	453-454	2	25
β-strand	456	1	26
β-strand	458	1	24
β-strand	461-467	7	21
α-helix	474-476	3
α-helix	478-480	3
α-helix	482-491	10
α-helix	503-514	12
α-helix	518-525	8
α-helix	534-545	12
β-strand	550-551	2	27
β-strand	557-558	2	27
β-strand	564-568	5	28
α-helix	576-584	9
β-strand	588-592	5	28
α-helix	598-602	5
β-strand	603-607	5	29
β-strand	614-618	5	29
α-helix	620-623	4
β-strand	628-632	5	28
α-helix	634-636	3
α-helix	639-643	5
α-helix	645-651	7
β-strand	653-658	6	30
β-strand	661-666	6	30
β-strand	670-674	5	28
α-helix	688-691	4
α-helix	696-699	4
β-strand	704-708	5	28
α-helix	714-726	13
α-helix	744-757	14
β-strand	761	1	31
α-helix	764-779	16
α-helix	795-810	16
α-helix	811-813	3
β-strand	816	1	31
α-helix	819-833	15
α-helix	835-837	3
β-strand	838	1	32
β-strand	845	1	32

Chain 5: 34 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	33
α-helix	18-20	3
α-helix	24-37	14
α-helix	45-56	12
β-strand	60-64	5	34
α-helix	65-71	7
α-helix	74-81	8
α-helix	83-107	25
α-helix	134-135	2
β-strand	136-140	5	34
α-helix	148-150	3
α-helix	153-155	3
β-strand	159-169	11	35
β-strand	173-183	11	35
β-strand	189-193	5	35
α-helix	220-225	6
α-helix	232-234	3
β-strand	241-243	3	35
α-helix	245-247	3
β-strand	249-259	11	35
α-helix	262-264	3
α-helix	265-266	2
α-helix	270-272	3
β-strand	273-279	7	35
α-helix	280-282	3
β-strand	291-299	9	35
β-strand	323	1	2
β-strand	327-335	9	35
α-helix	351-362	12
α-helix	366-373	8
α-helix	382-393	12
β-strand	398-399	2	36
β-strand	405-406	2	36
β-strand	412-416	5	37
α-helix	424-432	9
β-strand	436-440	5	37
β-strand	444	1	38
β-strand	447	1	38
β-strand	452-455	4	39
β-strand	462-466	5	39
α-helix	468-471	4
β-strand	475-480	6	37
α-helix	487-499	13
β-strand	503-505	3	40
β-strand	510-512	3	40
β-strand	517-523	7	37
α-helix	526-528	3
α-helix	536-539	4
α-helix	544-548	5
β-strand	552-556	5	37
α-helix	562-577	16
α-helix	579-582	4
α-helix	585-588	4
α-helix	596-609	14
α-helix	616-639	24
α-helix	650-665	16
α-helix	674-687	14
α-helix	688-693	6
α-helix	695-697	3

Chain 6: 29 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	101-103	3
α-helix	106-121	16
α-helix	135-146	12
β-strand	150-154	5	41
α-helix	155-159	5
α-helix	162-164	3
α-helix	165-172	8
α-helix	174-192	19
β-strand	264-268	5	41
α-helix	276-278	3
α-helix	281-283	3
β-strand	287-297	11	4
β-strand	301-311	11	4
β-strand	317-322	6	4
β-strand	344-346	3	4
α-helix	348-350	3
β-strand	352-362	11	4
α-helix	365-367	3
α-helix	368-369	2
α-helix	373-375	3
β-strand	376-382	7	4
α-helix	383-385	3
β-strand	394-405	12	4
α-helix	407-409	3
β-strand	418-421	4	22
β-strand	446-447	2	22
β-strand	450-461	12	4
α-helix	512-520	9
α-helix	525-532	8
α-helix	541-547	7
β-strand	557-558	2	42
β-strand	564-565	2	42
β-strand	571-574	4	43
α-helix	582-591	10
β-strand	595-599	5	43
β-strand	603	1	30
β-strand	610-613	4	44
β-strand	622-625	4	44
β-strand	635-639	5	43
α-helix	641-643	3
α-helix	646-657	12
β-strand	660-665	6	45
β-strand	668-673	6	45
β-strand	677-682	6	43
α-helix	695-698	4
α-helix	703-706	4
β-strand	711-714	4	43
α-helix	721-737	17
α-helix	738-740	3
α-helix	748-758	11
β-strand	764	1	46
α-helix	767-784	18
α-helix	797-814	18
β-strand	818	1	46
α-helix	821-833	13

Chain 7: 35 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	12
α-helix	14-27	14
α-helix	62-71	10
β-strand	77-81	5	47
α-helix	82-94	13
α-helix	102-108	7
α-helix	110-122	13
α-helix	138-164	27
α-helix	179-188	10
α-helix	194-196	3
β-strand	201-205	5	47
α-helix	209-215	7
β-strand	225	1	26
α-helix	232-234	3
β-strand	238-248	11	26
α-helix	249-251	3
β-strand	252-261	10	26
β-strand	267-272	6	26
β-strand	277-278	2	25
β-strand	283	1	48
α-helix	287-291	5
α-helix	295-296	2
β-strand	297	1	48
α-helix	298	1
β-strand	299-300	2	26
β-strand	306-316	11	26
α-helix	319-321	3
α-helix	323	1
β-strand	330-336	7	26
α-helix	337-339	3
β-strand	348-358	11	26
α-helix	364-366	3
β-strand	371	1	12
β-strand	373-383	11	26
α-helix	395-407	13
α-helix	410-415	6
α-helix	426-437	12
β-strand	442-445	4	49
β-strand	448-450	3	49
β-strand	456-460	5	50
α-helix	466-476	11
β-strand	481-484	4	50
α-helix	485-487	3
β-strand	488	1	17
α-helix	490-492	3
β-strand	495-499	5	51
β-strand	506-510	5	51
α-helix	512-515	4
β-strand	520-524	5	50
α-helix	526-528	3
α-helix	531-543	13
β-strand	545-550	6	52
β-strand	553-558	6	52
β-strand	562-567	6	50
α-helix	588-593	6
β-strand	596-600	5	50
α-helix	606-621	16
α-helix	632-634	3
α-helix	635-645	11
β-strand	651	1	53
α-helix	654-676	23
α-helix	686-703	18
β-strand	707	1	53
α-helix	710-722	13
α-helix	725-728	4

Chain A: 27 helices, 25 β-strands

Element	Residues	Length	Sheet
α-helix	194-198	5
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-245	5	54
α-helix	246-262	17
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-298	5	54
β-strand	304	1	55
α-helix	311-313	3
β-strand	316-327	12	55
β-strand	331-341	11	55
β-strand	347-352	6	55
β-strand	373-375	3	55
β-strand	381-391	11	55
α-helix	398	1
β-strand	405-411	7	55
α-helix	412-414	3
β-strand	423-432	10	55
β-strand	446	1	56
β-strand	449-458	10	55
α-helix	478-489	12
α-helix	493-498	6
α-helix	509-520	12
β-strand	525-526	2	57
β-strand	532-533	2	57
β-strand	539-543	5	58
α-helix	549-559	11
β-strand	563-567	5	58
β-strand	579-582	4	59
β-strand	589-592	4	59
α-helix	595-598	4
β-strand	602-607	6	58
α-helix	609-611	3
α-helix	614-626	13
β-strand	628-633	6	60
β-strand	636-641	6	60
β-strand	644-650	7	58
α-helix	653-655	3
α-helix	663-665	3
α-helix	671-674	4
β-strand	679-683	5	58
α-helix	689-706	18
α-helix	757-759	3
α-helix	760-773	14
β-strand	777	1	61
α-helix	783-800	18
α-helix	804-805	2
α-helix	807-823	17
β-strand	828	1	61
α-helix	831-847	17
α-helix	851-860	10

6 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2, A	protein	868	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3, B	protein	1006	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4, C	protein	933	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5, D	protein	775	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6, E	protein	1017	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P53091
DNA replication licensing factor MCM7	7, F	protein	845	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P38132
Cell division control protein 7	1	protein	507	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P06243
DDK kinase regulatory subunit DBF4	G	protein	738	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P32325
DNA (53-mer)	X	DNA	53	Saccharomyces cerevisiae S288C
DNA (53-mer)	Y	DNA	53	Saccharomyces cerevisiae S288C

Sequence of entity 1 (2, A), FASTA

>7P5Z_1 DNA replication licensing factor MCM2 (chains 2, A)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3, B), FASTA

>7P5Z_2 DNA replication licensing factor MCM3 (chains 3, B)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV

Sequence of entity 3 (4, C), FASTA

>7P5Z_3 DNA replication licensing factor MCM4 (chains 4, C)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5, D), FASTA

>7P5Z_4 Minichromosome maintenance protein 5 (chains 5, D)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6, E), FASTA

>7P5Z_5 DNA replication licensing factor MCM6 (chains 6, E)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7, F), FASTA

>7P5Z_6 DNA replication licensing factor MCM7 (chains 7, F)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (1), FASTA

>7P5Z_7 Cell division control protein 7 (chains 1)
MTSKTKNIDDIPPEIKEEMIQLYHDLPGIENEYKLIDKIGEGTFSSVYKAKDITGKITKK
FASHFWNYGSNYVALKKIYVTSSPQRIYNELNLLYIMTGSSRVAPLCDAKRVRDQVIAVL
PYYPHEEFRTFYRDLPIKGIKKYIWELLRALKFVHSKGIIHRDIKPTNFLFNLELGRGVL
VDFGLAEAQMDYKSMISSQNDYDNYANTNHDGGYSMRNHEQFCPCIMRNQYSPNSHNQTP
PMVTIQNGKVVHLNNVNGVDLTKGYPKNETRRIKRANRAGTRGFRAPEVLMKCGAQSTKI
DIWSVGVILLSLLGRRFPMFQSLDDADSLLELCTIFGWKELRKCAALHGLGFEASGLIWD
KPNGYSNGLKEFVYDLLNKECTIGTFPEYSVAFETFGFLQQELHDRMSIEPQLPDPKTNM
DAVDAYELKKYQEEIWSDHYWCFQVLEQCFEMDPQKRSSAEDLLKTPFFNELNENTYLLD
GESTDEDDVVSSSEADLLDKDVLLISE

Sequence of entity 8 (G), FASTA

>7P5Z_8 DDK kinase regulatory subunit DBF4 (chains G)
KRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMVSPTKMIIASPAKETDTNLKHNNGI
AASTTAAGHLNVFSNDNNCNNNNTTESFPKKRSLEALEAQQQQHLHEKKRARIERARSIE
GAVQVSKGTGLKNVEPRVTPKELLEWQTNWKKIMKRDSRIYFDITDDVEMNTYNKSKMDK
RRDLLKRGFLTLGAQITQFFDTTVTIVITRRSVENIYLLKDTDILSRAKKNYMKVWSYEK
AARFLKNLDVDLDHLSKTKSASLAAPTLSNLLHNEKLYGPTDRDPRTKRDDIHYFKYPHV
YLYDLWQTWAPIITLEWKPQELTNLDELPYPILKIGSFGRCPFIGDRNYDESSYKRVVKR
YSRDKANKKYALQLRALFQYHADTLLNTSSVNDQTKNLIFIPHTCNDSTKSFKKWMQEKA
KNFEKTELKKTDDSAVQDVRNEHADQTDEKNSILLNETETKEPPLKEEKENKQSIAEESN
KYPQRKELAATPKLNHPVLATFARQETEEVPDDLCTLKTKSRQAFEIKASGAHQSNDVAT
SFGNGLGPTRASVMSKNMKSLSRLMVDRKLGVKQTNGNNKNYTATIATTAETSKENRHRL
DFNALKKDEAPSKETGKDSAVHLETNRKPQNFPKVATKSVSADSKVHNDIKITTTESPTA
SKKSTSTNVTLHFNAQTAQTAQPVKKETVKNSGYCENCRVKYESLEQHIVSEKHLSFAEN
DLNFEAIDSLIENLRFQI

Sequence of entity 9 (X), FASTA

>7P5Z_9 DNA (53-MER) (chains X)
GCATGCATGCGCATGCATGCATGCATGCTGCATGCATGCATGCGCATGCATGC

Sequence of entity 10 (Y), FASTA

>7P5Z_10 DNA (53-MER) (chains Y)
GCATGCATGCGCATGCATGCATGCAGCATGCATGCATGCATGCGCATGCATGC

Ligands and cofactors

ID	Name	Formula	Copies
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	8
ZN	Zinc ion	Zn	11
MG	Magnesium ion	Mg	10
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2

Primary citation

Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase. Greiwe, J.F., Miller, T.C.R., Locke, J. et al. Nat Struct Mol Biol (2022) 29:10-20. DOI 10.1038/s41594-021-00698-z · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

Browse structure collections

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