Histone H3-like centromeric protein CSE4 (CSE4) is a 229-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P36012.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 71.8 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 35% |
| 70 to 90 | Confident: backbone generally right | 21% |
| 50 to 70 | Low: treat with caution | 15% |
| Below 50 | Very low: often disordered regions | 28% |
What pLDDT means and how to read it
Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation
Component of centromeric nucleosomes, where DNA is wrapped around a histone octamer core (PubMed:14581449, PubMed:32004465). The octamer contains two molecules each of H2A, H2B, CSE4/CENPA and H4 assembled in one CSE4-H4 heterotetramer and two H2A-H2B heterodimers (PubMed:14581449, PubMed:32004465). Interacts with the inner kinetochore (PubMed:14581449). Interacts with the central kinetochore…
Nucleus, Chromosome, centromere
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 8T0P | X-ray | 1.73 Å | C=32-49 |
| 6UPH | EM | 2.7 Å | A/E=1-229 |
| 7ON1 | EM | 3.35 Å | a/e=1-229 |
| 8OW0 | EM | 3.4 Å | a/e=1-229 |
| 8OW1 | EM | 3.7 Å | a/e=1-229 |
| 6QLD | EM | 4.15 Å | a=137-226, e=112-226 |
| 2L5A | NMR | A=151-228 | |
| 2LY8 | NMR | A=152-212 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.