Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) is a 205-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q13257.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 93.8 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 90% |
| 70 to 90 | Confident: backbone generally right | 5% |
| 50 to 70 | Low: treat with caution | 2% |
| Below 50 | Very low: often disordered regions | 2% |
What pLDDT means and how to read it
Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle (PubMed:10700282, PubMed:11804586, PubMed:15024386, PubMed:29162720, PubMed:27509861). The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint (PubMed:15020684, PubMed:27509861). The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition (PubMed:27509861). When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby…
Monomer and homodimer (PubMed:18022367, PubMed:18318601). Component of the mitotic checkpoint complex (MCC), composed of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3; within the complex interacts with CDC20 (PubMed:29162720, PubMed:27509861). The MCC associates with CDC20-APC/C to form the CDC20-APC/C-MCC complex; within the complex BUB1B/BUBR1 interacts with both the CDC20 in MCC and the CDC20 in…
Nucleus, Chromosome, centromere, kinetochore, Cytoplasm, Cytoplasm, cytoskeleton, spindle pole
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2VFX | X-ray | 1.95 Å | A/B/C/D/E/F/G/H/I/J/K/L=1-205 |
| 1GO4 | X-ray | 2.05 Å | A/B/C/D=1-205 |
| 2QYF | X-ray | 2.3 Å | A/C=1-205 |
| 2V64 | X-ray | 2.9 Å | A/C/D/E/F/H=2-205 |
| 6TLJ | EM | 3.8 Å | Z=1-205 |
| 3GMH | X-ray | 3.95 Å | A/B/C/D/E/F/G/H/I/J/K/L=11-205 |
| 5LCW | EM | 4.0 Å | Z=1-205 |
| 6F0X | EM | 4.6 Å | Z=1-205 |
| 5KHU | EM | 4.8 Å | T=1-205 |
| 1DUJ | NMR | A=11-195 | |
| 1KLQ | NMR | A=11-205 | |
| 1S2H | NMR | A=1-205 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.