6TLJ: Anaphase-promoting complex/Cyclosome

Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the Mitotic checkpoint complex (APC/C-MCC) at 3.8 angstrom resolution. Determined by electron microscopy at 3.8 Å resolution. Released 19 Feb 2020.

Method
Electron microscopy
Resolution
3.8 Å
Organism
Homo sapiens
Chains
23
Atoms
72,305
Mol. weight
1411.71 kDa
Released
19 Feb 2020

Explore 6TLJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6TLJ contains 446 α-helices and 185 β-strands across 23 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 64 helices, 49 β-strands

ElementResiduesLengthSheet
β-strand1311
β-strand18-1922
α-helix23-286
α-helix40-456
β-strand73-7863
β-strand90-9563
β-strand99-10353
β-strand113-11863
β-strand127-13264
β-strand147-15484
β-strand159-16354
β-strand170-17124
β-strand17715
β-strand181-18226
β-strand187-18826
β-strand19115
β-strand211-21336
α-helix219-2202
β-strand221-22226
β-strand224-22527
β-strand237-23827
β-strand244-25078
β-strand255-26068
β-strand266-27278
α-helix351-3599
β-strand408-41368
β-strand430-43349
β-strand440-44459
β-strand452-45879
β-strand467-47489
β-strand478-482510
β-strand487-492610
β-strand496-500510
β-strand505-508410
β-strand51011
β-strand584-59182
β-strand594-59962
β-strand604-60852
α-helix609-6113
α-helix616-62813
α-helix632-64615
α-helix656-66611
α-helix759-77618
α-helix782-79716
α-helix801-8099
α-helix844-85310
α-helix868-88114
α-helix925-93612
α-helix941-9444
α-helix949-96315
α-helix972-9776
α-helix1021-10277
α-helix1033-10419
α-helix10461
β-strand1047111
α-helix10481
α-helix1059-107719
α-helix1081-10877
β-strand1092112
α-helix1093-10953
α-helix1101-11044
β-strand1109-1111311
β-strand1116-1118311
α-helix1133-114513
β-strand1147112
α-helix1155-11606
α-helix1169-118214
α-helix1185-11873
α-helix1190-11989
α-helix1202-121615
α-helix1222-12298
α-helix1244-12463
α-helix1247-126014
α-helix1267-127711
α-helix1291-130616
α-helix1321-133010
β-strand1352-1353213
α-helix1360-137415
α-helix1381-13855
α-helix1395-13973
α-helix1401-141414
α-helix1423-14275
α-helix1432-14365
α-helix1454-147623
α-helix1482-149918
α-helix1504-15074
α-helix1509-152618
α-helix1533-154412
α-helix1552-156615
β-strand1572114
α-helix1579-158810
α-helix1591-15933
α-helix1604-16129
β-strand1616114
β-strand1620-1623415
β-strand1628-1629215
β-strand1633-1638616
β-strand1648-1652516
α-helix1660-16623
β-strand1663-1669716
β-strand1675-1678416
α-helix1685-16928
β-strand1696-1699415
α-helix1745-17506
α-helix1751-17555
α-helix1765-178016
α-helix1787-180216
α-helix1809-182214
α-helix1845-186218
α-helix1865-18717
α-helix1881-189212
α-helix1913-19219
α-helix1928-19358
Chain B: 3 helices, 5 β-strands
ElementResiduesLengthSheet
β-strand7117
α-helix33-342
β-strand46-47218
β-strand55-56218
α-helix57-648
β-strand72119
β-strand79119
α-helix80-812
Chain C: 33 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix29-4618
α-helix49-6113
α-helix63-653
α-helix71-766
α-helix78-825
α-helix84-9613
α-helix99-1068
α-helix112-13625
α-helix151-16313
α-helix170-18112
α-helix185-19814
α-helix203-21210
α-helix216-2216
α-helix229-24113
α-helix244-25714
α-helix259-2613
α-helix263-27513
α-helix279-29214
α-helix300-30910
α-helix313-32614
α-helix331-34313
α-helix347-36014
α-helix366-37712
α-helix381-39414
α-helix399-41113
α-helix415-42814
α-helix433-44614
α-helix449-46214
α-helix469-47911
α-helix483-49917
α-helix510-52213
α-helix526-53914
α-helix544-55613
Chain D: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix9-113
Chain E: 3 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix54-9138
α-helix93-953
α-helix99-1024
Chain F: 30 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix7-1610
α-helix21-3515
α-helix38-5013
α-helix54-6310
α-helix69-8113
α-helix85-939
α-helix104-1107
α-helix111-1133
α-helix114-12714
α-helix131-14414
α-helix150-1578
α-helix166-1683
α-helix452-47726
α-helix481-4899
α-helix499-51214
α-helix515-52814
α-helix536-54611
α-helix549-56214
α-helix567-57913
α-helix583-59614
α-helix601-61313
α-helix617-63014
α-helix635-64713
α-helix651-66414
α-helix669-68113
α-helix685-69814
α-helix703-71513
α-helix719-73214
α-helix737-75014
α-helix753-76513
Chain G: 2 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix4-74
α-helix13-2311
Chain H: 30 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix7-1711
α-helix21-3515
α-helix38-5013
α-helix54-6310
α-helix69-8113
α-helix85-939
α-helix104-1107
α-helix111-1133
α-helix114-12714
α-helix131-14414
α-helix149-1579
α-helix165-1684
α-helix452-47726
α-helix481-4899
α-helix499-51214
α-helix515-52814
α-helix536-54611
α-helix549-56214
α-helix567-58014
α-helix583-59614
α-helix601-61414
α-helix617-63014
α-helix635-64713
α-helix651-66414
α-helix669-68113
α-helix685-69814
α-helix703-71513
α-helix719-73214
α-helix737-75014
α-helix753-76614

15 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Anaphase-promoting complex subunit 1Aprotein1944Homo sapiensQ9H1A4 (AlphaFold model)
Anaphase-promoting complex subunit 11Bprotein84Homo sapiensQ9NYG5 (AlphaFold model)
Cell division cycle protein 23 homologC, Pprotein597Homo sapiensQ9UJX2 (AlphaFold model)
Anaphase-promoting complex subunit 15Dprotein121Homo sapiensP60006 (AlphaFold model)
Anaphase-promoting complex subunit 16Eprotein110Homo sapiensQ96DE5
Cell division cycle protein 27 homologF, Hprotein824Homo sapiensP30260
Anaphase-promoting complex subunit CDC26G, Wprotein85Homo sapiensQ8NHZ8
Anaphase-promoting complex subunit 4Iprotein808Homo sapiensQ9UJX5
Cell division cycle protein 16 homologJ, Kprotein620Homo sapiensQ13042
Anaphase-promoting complex subunit 10Lprotein185Homo sapiensQ9UM13
Anaphase-promoting complex subunit 13Mprotein74Homo sapiensQ9BS18
Anaphase-promoting complex subunit 2Nprotein822Homo sapiensQ9UJX6

6 more molecules are not listed.

Sequence of entity 1 (A), FASTA
>6TLJ_1 Anaphase-promoting complex subunit 1 (chains A)
MSNFYEERTTMIAARDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGS
LQEVTIHEKQKESWQLRKGVSEIGEDVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVD
SPVQQALWCDFIISQDKSEKAYSSNEVEKCICILQSSCINMHSIEGKDYIASLPFQVANV
WPTKYGLLFERSASSHEVPPGSPREPLPTMFSMLHPLDEITPLVCKSGSLFGSSRVQYVV
DHAMKIVFLNTDPSIVMTYDAVQNVHSVWTLRRVKSEEENVVLKFSEQGGTPQNVATSSS
LTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAALSRAHSFALGV
HSFSGVQRFNISSHNQSPKRHSISHSPNSNSNGSFLAPETEPIVPELCIDHLWTETITNI
REKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNIPAKDAA
PVEKIDTMLVLEGSGNLVLYTGVVRVGKVFIPGLPAPSLTMSNTMPRPSTPLDGVSTPKP
LSKLLGSLDEVVLLSPVPELRDSSKLHDSLYNEDCTFQQLGTYIHSIRDPVHNRVTLELS
NGSMVRITIPEIATSELVQTCLQAIKFILPKEIAVQMLVKWYNVHSAPGGPSYHSEWNLF
VTCLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSETGSDDDWEYLLNSDYHQNVE
SHLLNRSLCLSPSEASQMKDEDFSQNLSLDSSTLLFTHIPAIFFVLHLVYEELKLNTLMG
EGICSLVELLVQLARDLKLGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGFMHHPSFFTSE
PPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYILGDESLVSDESSQYLTRIT
IAPQKLQVEQEENRFSFRHSTSVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIRDAIY
HCREQPASDWPEAVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMN
HEVMSLIWSEDLRVQDVRRLLQSAHPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMAL
PVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLNSGNIDVPPNMTSWASFHNG
VAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKG
HEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLV
YQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLN
VPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNN
RSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSIS
LSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKDFMTYLS
APNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNYGFHLAHHM
ALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLYVLAAEPRLLV
PVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDL
SKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISA
FTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRL
GRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWL
QVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHLPPIGLEGSTSFAELLFKFKQ
LKMPVRALLRLAPLLLGNPQPMVM
Sequence of entity 2 (B), FASTA
>6TLJ_2 Anaphase-promoting complex subunit 11 (chains B)
MKVKIKCWNGVATWLWVANDENCGICRMAFNGCCPDCKVPGDDCPLVWGQCSHCFHMHCI
LKWLHAQQVQQHCPMCRQEWKFKE
Sequence of entity 3 (C, P), FASTA
>6TLJ_3 Cell division cycle protein 23 homolog (chains C, P)
MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAF
SLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMY
SRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLR
KLDLVKEAIDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTE
LQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENM
DTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKL
NPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLY
YYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQL
TESEQAAQCYIKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFN
DTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP
Sequence of entity 4 (D), FASTA
>6TLJ_4 Anaphase-promoting complex subunit 15 (chains D)
MSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSIAEKDNNLVPIGKPASE
HYDDEEEEDDEDDEDSEEDSEDDEDMQDMDEMNDYNESPDDGEVNEVDMEGNEQDQDQWM
I
Sequence of entity 5 (E), FASTA
>6TLJ_5 Anaphase-promoting complex subunit 16 (chains E)
MAASSSSSSAGGVSGSSVTGSGFSVSDLAPPRKALFTYPKGAGEMLEDGSERFLCESVFS
YQVASTLKQVKHDQQVARMEKLAGLVEELEADEWRFKPIEQLLGFTPSSG
Sequence of entity 6 (F, H), FASTA
>6TLJ_6 Cell division cycle protein 27 homolog (chains F, H)
MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRL
LKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSL
LGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCL
PNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVI
SPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQN
YTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTP
QVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQ
PNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYN
CKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYS
TTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNY
AYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQK
ALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYK
SALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAID
KRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
Sequence of entity 7 (G, W), FASTA
>6TLJ_7 Anaphase-promoting complex subunit CDC26 (chains G, W)
MLRRKPTRLELKLDDIEEFENIRKDLETRKKQKEDVEVVGGSDGEGAIGLSSDPKSREQM
INDRIGYKPQPKPNNRSSQFGSLEF
Sequence of entity 8 (I), FASTA
>6TLJ_8 Anaphase-promoting complex subunit 4 (chains I)
MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPP
NENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTV
ESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGG
SSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLSVVTEVSTNGASEVSYFQLETNL
LYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEILMQMDSRLTKFVQEKNTTTSV
QDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVISHLQSGSESLL
YHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRW
LYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLK
DEDDDLVSPPNTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKP
ADVIGKSMNQAICIPLYRDTRSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCI
LRRHTDISQSVSNGLIAIKFGSFTYATTEKVRRSIYSCLDAQFYDDETVTVVLKDTVGRE
GRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQ
YVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESE
AENQQAGAAALAPEIVIKVEKLDPELDS
Sequence of entity 9 (J, K), FASTA
>6TLJ_9 Cell division cycle protein 16 homolog (chains J, K)
MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHAL
RSRKLDKLYEACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSD
WEMSQSSIKSSICLLRGKIYDALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQE
EKELLESLPLSKLCNEEQELLRFLFENKLKKYNKPSETVIPESVDGLQENLDVVVSLAER
HYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNKANELFYLSHKLVDLYPSNPV
SWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEHDQAMAAYFTA
AQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKT
AEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNAS
TYSAIGYIHSLMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKD
KLKCYDFDVHTMKTLKNIISPPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETF
EIEMNESDMMLETSMSDHST
Sequence of entity 10 (L), FASTA
>6TLJ_10 Anaphase-promoting complex subunit 10 (chains L)
MTTPNKTPPGADPKQLERTGTVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGSQ
PHLVNIQFRRKTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWI
HVPLTDNHKKPTRTFMIQIAVLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMM
YRSIR
Sequence of entity 11 (M), FASTA
>6TLJ_11 Anaphase-promoting complex subunit 13 (chains M)
MDSEVQRDGRILDLIDDAWREDKLPYEDVAIPLNELPEPEQDNGGTTESVKEQEMKWTDL
ALQYLHENVPPIGN
Sequence of entity 12 (N), FASTA
>6TLJ_12 Anaphase-promoting complex subunit 2 (chains N)
MAAAVVVAEGDSDSRPGQELLVAWNTVSTGLVPPAALGLVSSRTSGAVPPKEEELRAAVE
VLRGHGLHSVLEEWFVEVLQNDLQANISPEFWNAISQCENSADEPQCLLLLLDAFGLLES
RLDPYLRSLELLEKWTRLGLLMGTGAQGLREEVHTMLRGVLFFSTPRTFQEMIQRLYGCF
LRVYMQSKRKGEGGTDPELEGELDSRYARRRYYRLLQSPLCAGCSSDKQQCWCRQALEQF
HQLSQVLHRLSLLERVSAEAVTTTLHQVTRERMEDRCRGEYERSFLREFHKWIERVVGWL
GKVFLQDGPARPASPEAGNTLRRWRCHVQRFFYRIYASLRIEELFSIVRDFPDSRPAIED
LKYCLERTDQRQQLLVSLKAALETRLLHPGVNTCDIITLYISAIKALRVLDPSMVILEVA
CEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPASLETGQDSEDDSGEPEDW
VPDPVDADPGKSSSKRRSSDIISLLVSIYGSKDLFINEYRSLLADRLLHQFSFSPEREIR
NVELLKLRFGEAPMHFCEVMLKDMADSRRINANIREEDEKRPAEEQPPFGVYAVILSSEF
WPPFKDEKLEVPEDIRAALEAYCKKYEQLKAMRTLSWKHTLGLVTMDVELADRTLSVAVT
PVQAVILLYFQDQASWTLEELSKAVKMPVALLRRRMSVWLQQGVLREEPPGTFSVIEEER
PQDRDNMVLIDSDDESDSGMASQADQKEEELLLFWTYIQAMLTNLESLSLDRIYNMLRMF
VVTGPALAEIDLQELQGYLQKKVRDQQLVYSAGVYRLPKNCS

Primary citation

A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase. Alfieri, C., Tischer, T., Barford, D. EMBO Rep (2020) 21:e49831-e49831. DOI 10.15252/embr.201949831 · PubMed

Other PDB entries of the same protein (UniProt Q9H1A4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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