Structure-specific endonuclease subunit MUS81 (mus81) is a 604-residue protein from Danio rerio. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q7SXA9.
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The mean pLDDT of this model is 75.1 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 37% |
| 70 to 90 | Confident: backbone generally right | 34% |
| 50 to 70 | Low: treat with caution | 7% |
| Below 50 | Very low: often disordered regions | 21% |
What pLDDT means and how to read it
Catalytic subunit of two functionally distinct, structure-specific, heterodimeric DNA endonucleases MUS81-EME1 and MUS81-EME2 that are involved in the maintenance of genome stability. Both endonucleases have essentially the same substrate specificity though MUS81-EME2 is more active than its MUS81-EME1 counterpart. Both cleave 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs. MUS81-EME2 which is active during the replication of DNA is more specifically involved in replication fork processing. Replication forks frequently encounter obstacles to their passage, including DNA base lesions, DNA interstrand…
Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME1. Part of the heterodimeric DNA structure-specific endonuclease complex MUS81-EME2
Nucleus, nucleolus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 2ZIU | X-ray | 2.7 Å | A=295-604 |
| 2ZIV | X-ray | 2.7 Å | A=295-604 |
| 2ZIW | X-ray | 2.8 Å | A=295-604 |
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