Outer kinetochore KNL1 complex subunit KNL1 (KNL1) is a 2342-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q8NG31.
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The mean pLDDT of this model is 39.8 (very low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 1% |
| 70 to 90 | Confident: backbone generally right | 15% |
| 50 to 70 | Low: treat with caution | 2% |
| Below 50 | Very low: often disordered regions | 82% |
What pLDDT means and how to read it
Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15502821, PubMed:17981135, PubMed:18045986, PubMed:19893618, PubMed:21199919, PubMed:22000412, PubMed:22331848, PubMed:27881301, PubMed:30100357). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:18045986, PubMed:19893618, PubMed:27881301). The outer kinetochore is made up of the ten-subunit KMN network,…
Component of the KNL1 complex composed of KNL1 and ZWINT (Probable) (PubMed:24530301, PubMed:27881301, PubMed:38459127, PubMed:38459128). Part of the ten-subunit outer kinetochore KMN network that includes the KNL1, MIS12 and NDC80 complexes; a bioriented kinetochore contains approximately 150 copies of the network (PubMed:24530301, PubMed:38459127, PubMed:38459128). Interacts (via C-terminus)…
Nucleus, Chromosome, centromere, kinetochore, Cytoplasm
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3SI5 | X-ray | 2.2 Å | X/Y=234-252 |
| 4NFA | X-ray | 2.5 Å | A=2131-2337 |
| 4A1G | X-ray | 2.6 Å | E/F/G/H=176-226 |
| 4NF9 | X-ray | 2.8 Å | A/B=2117-2337 |
| 6CZO | X-ray | 2.95 Å | B/D=23-80 |
| 8PPR | EM | 3.0 Å | K=1-2342 |
| 8Q5H | EM | 4.5 Å | K=2021-2342 |
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