DNA topoisomerase 2-binding protein 1 (TOPBP1) is a 1522-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q92547.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 66.1 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 26% |
| 70 to 90 | Confident: backbone generally right | 30% |
| 50 to 70 | Low: treat with caution | 7% |
| Below 50 | Very low: often disordered regions | 37% |
What pLDDT means and how to read it
Scaffold protein that acts as a key protein-protein adapter in DNA replication and DNA repair (PubMed:10498869, PubMed:11395493, PubMed:11714696, PubMed:17575048, PubMed:20545769, PubMed:21777809, PubMed:26811421, PubMed:30898438, PubMed:31135337, PubMed:33592542, PubMed:35597237, PubMed:37674080). Composed of multiple BRCT domains, which specifically recognize and bind phosphorylated proteins, bringing proteins together into functional combinations (PubMed:17575048, PubMed:20545769, PubMed:21777809, PubMed:26811421, PubMed:30898438, PubMed:31135337, PubMed:35597237, PubMed:37674080). Required for DNA replication initiation but not for the formation of pre-replicative complexes or the…
Interacts (via BRCT domains 1 and 2) with (phosphorylated) MDC1; promoting TOPBP1 recruitment to DNA damage sites during mitosis (PubMed:21482717, PubMed:23891287, PubMed:30898438). Interacts (via BRCT domains 7 and 8) with (autophosphorylated) ATR; promoting activation of ATR (PubMed:21777809). Interacts (via BRCT domains 7 and 8) with (phosphorylated) POLQ; specifically binds POLQ…
Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3PD7 | X-ray | 1.26 Å | A/B=893-994 |
| 3JVE | X-ray | 1.34 Å | A=893-996 |
| 9MAT | X-ray | 1.65 Å | A=1264-1493 |
| 7CMZ | X-ray | 1.7 Å | A=1264-1493 |
| 3UEN | X-ray | 1.9 Å | A=549-746 |
| 3AL2 | X-ray | 2.0 Å | A=1264-1493 |
| 3AL3 | X-ray | 2.15 Å | A=1264-1493 |
| 3OLC | X-ray | 2.4 Å | X=1-290 |
| 9MAW | X-ray | 2.45 Å | A=1264-1491 |
| 2XNK | X-ray | 2.6 Å | A/B/C/D=1-290 |
| 3UEO | X-ray | 2.6 Å | A/B/C/D=549-746 |
| 2XNH | X-ray | 2.8 Å | A=1-287 |
| 6RML | X-ray | 2.81 Å | A/B=1-290 |
| 6RMM | X-ray | 3.53 Å | A/B/C/D=548-741 |
| 8OK2 | EM | 4.1 Å | E=331-766 |
| 1WF6 | NMR | A=326-444 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.