FACT complex subunit SPT16 (SUPT16H) is a 1047-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9Y5B9.
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The mean pLDDT of this model is 79.9 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 48% |
| 70 to 90 | Confident: backbone generally right | 31% |
| 50 to 70 | Low: treat with caution | 7% |
| Below 50 | Very low: often disordered regions | 14% |
What pLDDT means and how to read it
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of…
Interacts with MYOG (via C-terminal region) (By similarity). Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H (PubMed:10421373). Also a component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B (PubMed:11239457, PubMed:12393879). Interacts with NEK9 (PubMed:14660563). Binds to histone H2A-H2B…
Nucleus, Chromosome
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5E5B | X-ray | 1.84 Å | A=2-432 |
| 5UMU | X-ray | 1.9 Å | A/B=649-926 |
| 4Z2N | X-ray | 1.92 Å | A=644-930 |
| 8I17 | X-ray | 1.98 Å | C/F/I=926-965 |
| 5UMT | X-ray | 2.09 Å | A=1-434 |
| 5XM2 | X-ray | 2.19 Å | A/B=1-437 |
| 4Z2M | X-ray | 2.98 Å | B=644-930 |
| 9EH2 | EM | 3.1 Å | x=1-1047 |
| 9RZC | EM | 3.66 Å | k=1-1047 |
| 8YJM | X-ray | 4.15 Å | A=644-988 |
| 8YJF | X-ray | 4.4 Å | A=644-988 |
| 9GW2 | EM | 4.84 Å | j=1-1047 |
| 6UPK | EM | 4.9 Å | G=2-925 |
| 9S3G | EM | 6.4 Å | j=1-1047 |
| 9S0U | EM | 6.72 Å | k=1-1047 |
| 6UPL | EM | 7.4 Å | G=2-925 |
| 9RZE | EM | 8.53 Å | j=1-1047 |
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