1H2V: Human nuclear cap-binding-complex

Structure of the human nuclear cap-binding-complex (CBC). Determined by X-ray diffraction at 2.0 Å resolution. Released 17 Oct 2002.

Method: X-ray diffraction
Resolution: 2.0 Å
Organism: HOMO SAPIENS
Chains: 2
Atoms: 6,961
Mol. weight: 107.69 kDa
Released: 17 Oct 2002

Explore 1H2V in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1H2V contains 53 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 50 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	30-36	7
α-helix	46-59	14
α-helix	61-78	18
α-helix	80-82	3
α-helix	83-94	12
α-helix	98-117	20
α-helix	121-136	16
β-strand	140	1	1
α-helix	142-154	13
α-helix	155-157	3
α-helix	163-174	12
α-helix	177-204	28
α-helix	211-214	4
β-strand	217	1	2
β-strand	227	1	3
α-helix	228-241	14
α-helix	252-256	5
α-helix	262-264	3
β-strand	266	1	1
α-helix	267-269	3
α-helix	273-276	4
α-helix	283-285	3
β-strand	287	1	3
α-helix	294-296	3
α-helix	302-305	4
α-helix	309-325	17
α-helix	329-337	9
α-helix	347-359	13
α-helix	369-382	14
α-helix	387-400	14
α-helix	402-404	3
β-strand	405	1	2
α-helix	407-421	15
α-helix	430-436	7
α-helix	444-458	15
α-helix	462-466	5
α-helix	471-476	6
α-helix	478-480	3
α-helix	498-509	12
α-helix	514-520	7
α-helix	521-523	3
α-helix	541-554	14
α-helix	559-568	10
α-helix	570-576	7
α-helix	580-594	15
α-helix	598-610	13
α-helix	616-623	8
α-helix	626-628	3
α-helix	635-661	27
α-helix	693-731	39
α-helix	738-753	16
α-helix	755-758	4
α-helix	759-761	3
α-helix	762-764	3
α-helix	765-769	5
α-helix	776-787	12

Chain Z: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	41-45	5	4
α-helix	53-60	8
α-helix	61-63	3
β-strand	66-73	8	4
β-strand	80-88	9	4
α-helix	91-100	10
β-strand	105-106	2	5
β-strand	109-110	2	5
β-strand	112-116	5	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
80 kda nuclear cap binding protein	C	protein	771	HOMO SAPIENS	Q09161 (AlphaFold model)
20 kda nuclear cap binding protein	Z	protein	156	HOMO SAPIENS	P52298 (AlphaFold model)

Sequence of entity 1 (C), FASTA

>1H2V_1 80 KDA NUCLEAR CAP BINDING PROTEIN (chains C)
KTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLL
PEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHV
IAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANT
ESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSI
LCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLH
CIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIEL
CKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLSQ
DPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPSNPTCIYKYGDESSNSLPGHS
VALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSF
SHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAV
ANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDR
SSDRKDGVLEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPW
YKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA

Sequence of entity 2 (Z), FASTA

>1H2V_2 20 KDA NUCLEAR CAP BINDING PROTEIN (chains Z)
MSGGLLKALRSDSYVELSQYRDQHFRGDNEEQEKLLKKSCTLYVGNLSFYTTEEQIYELF
SKSGDIKKIIMGLDKMKKTACGFCFVEYYSRADAENAMRYINGTRLDDRIIRTDWDAGFK
EGRQYGRGRSGGQVRDEYRQDYDAGRGGYGKLAQNQ

Primary citation

Large-Scale Induced Fit Recognition of an M(7)Gpppg CAP Analogue by the Human Nuclear CAP-Binding Complex. Mazza, C., Segref, A., Mattaj, I.W. et al. EMBO J (2002) 21:5548. DOI 10.1093/EMBOJ/CDF538 · PubMed

Other PDB entries of the same protein (UniProt Q09161 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1H6K 2.0 Å, nuclear Cap Binding Complex
1H2T 2.1 Å, Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue…
1N52 2.11 Å, Cap Binding Complex
3FEY 2.2 Å, Crystal structure of the CBC-importin alpha complex.
1H2U 2.4 Å, Structure of the human nuclear cap-binding-complex (CBC) in complex with a cap analogue…
9HFL 2.62 Å, Cryo-EM structure of the human snRNA export complex comprising CBC-PHAX-CRM1-RanGTP and…
6D0Y 2.68 Å, X-ray Crystal Structure of PGC-1beta C-terminus bound to the CBP80-CBP20 Cap Binding…
1N54 2.72 Å, Cap Binding Complex m7GpppG free
5OOB 2.79 Å, Complex of human nuclear cap-binding complex with M7GTP and nelf-E C-terminal peptide
5OO6 2.8 Å, Complex of human nuclear cap-binding complex with ARS2 C-terminal peptide
8BY6 3.19 Å, Structure of the human nuclear cap-binding complex bound to NCBP3(560-620) and…
8SRR 3.22 Å, Cryo-EM structure of the CBC-ALYREF complex

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