1H3O: Human TAF4-TAF12 (TAFII135-TAFII20) Complex

Crystal Structure of the Human TAF4-TAF12 (TAFII135-TAFII20) Complex. Determined by X-ray diffraction at 2.3 Å resolution. Released 26 Sept 2002.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: HOMO SAPIENS
Chains: 4
Atoms: 2,172
Mol. weight: 35.74 kDa
Released: 26 Sept 2002

Explore 1H3O in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1H3O contains 13 α-helices and 4 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	873-885	13
β-strand	891-892	2	1
α-helix	896-916	21

Chain B: 5 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	60-70	11
α-helix	75-77	3
α-helix	78-105	28
β-strand	110-111	2	1
α-helix	113-119	7
α-helix	120-124	5

Chain C: 2 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	873-887	15
β-strand	891-892	2	2
α-helix	895-916	22

Chain D: 4 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	60-68	9
α-helix	78-105	28
β-strand	110-111	2	2
α-helix	113-119	7
α-helix	120-124	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Transcription initiation factor tfiid 135 kda subunit	A, C	protein	75	HOMO SAPIENS	O00268 (AlphaFold model)
Transcription initiation factor tfiid 20/15 kda subunits	B, D	protein	76	HOMO SAPIENS	Q16514 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>1H3O_1 TRANSCRIPTION INITIATION FACTOR TFIID 135 KDA SUBUNIT (chains A, C)
MFLLQAPLQRRILEIGKKHGITELHPDVVSYVSHATQQRLQNLVEKISETAQQKNFSYKD
DDRYEQASDVRAQLK

Sequence of entity 2 (B, D), FASTA

>1H3O_2 TRANSCRIPTION INITIATION FACTOR TFIID 20/15 KDA SUBUNITS (chains B, D)
GSHMVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLE
VKDVQLHLERQWNMWI

Primary citation

Crystal Structure of a Subcomplex of Human Transcription Factor TFIID Formed by TATA Binding Protein-Associated Factors Htaf4 (Htaf(II)135) and Htaf12 (Htaf(II)20). Werten, S., Mitschler, A., Romier, C. et al. J Biol Chem (2002) 277:45502. DOI 10.1074/JBC.M206587200 · PubMed

Other PDB entries of the same protein (UniProt O00268 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2P6V 2.0 Å, Structure of TAFH domain of the human TAF4 subunit of TFIID
7EGG 2.77 Å, TFIID lobe B subcomplex
7EGF 3.16 Å, TFIID lobe A subcomplex
7EGB 3.3 Å, TFIID-based holo PIC on SCP promoter
7EG9 3.7 Å, TFIID-based intermediate PIC on SCP promoter
7EGC 3.9 Å, p53-bound TFIID-based holo PIC on HDM2 promoter
7ENA 4.07 Å, TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED)
7EGA 4.1 Å, TFIID-based intermediate PIC on PUMA promoter
7ENC 4.13 Å, TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)
8GXS 4.16 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
6MZC 4.5 Å, Human TFIID BC core
7EDX 4.5 Å, p53-bound TFIID-based core PIC on HDM2 promoter

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