1LPQ: DNA topoisomerase I

Human DNA Topoisomerase I (70 Kda) In Non-Covalent Complex With A 22 Base Pair DNA Duplex Containing an 8-oxoG Lesion. Determined by X-ray diffraction at 3.14 Å resolution. Released 16 Aug 2002.

Method
X-ray diffraction
Resolution
3.14 Å
Organism
Homo sapiens
Chains
3
Atoms
5,381
Mol. weight
80.28 kDa
Released
16 Aug 2002

Explore 1LPQ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1LPQ contains 34 α-helices and 23 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 34 helices, 23 β-strands

ElementResiduesLengthSheet
α-helix205-2073
α-helix209-2124
β-strand220-22121
β-strand22612
α-helix227-2315
α-helix233-2353
β-strand240-24233
β-strand245-24733
α-helix251-26111
α-helix267-2704
α-helix272-28413
α-helix288-2914
β-strand300-30123
α-helix303-31614
α-helix321-33717
β-strand340-34341
β-strand346-34941
β-strand35014
β-strand35412
α-helix355-3584
β-strand359-36025
α-helix3721
β-strand373-37425
α-helix375-3773
β-strand383-38426
β-strand403-40426
β-strand416-41727
β-strand424-42527
β-strand42914
α-helix437-45014
α-helix455-4628
α-helix475-48410
β-strand49518
β-strand49818
β-strand50819
α-helix509-5113
β-strand512-517610
β-strand522-530910
α-helix532-5343
β-strand536-542710
α-helix545-5539
β-strand56319
α-helix570-58011
α-helix586-60520
α-helix612-62211
α-helix625-6273
α-helix648-6525
α-helix653-66210
α-helix667-6726
α-helix680-6823
α-helix684-6874
α-helix689-69911
α-helix702-7076
α-helix726-73510
α-helix740-7434
α-helix746-7516
α-helix753-7575

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*TP*(8OG)P*GP*AP*AP*AP*AP*AP*TP*TP*TP*TP*T)-3'BDNA22
5'-d(*ap*ap*ap*ap*ap*tp*tp*tp*tp*tp*cp*cp*ap*ap*gp*tp*cp*tp*tp*tp*tp*t)-3'CDNA22
DNA topoisomerase IAprotein564Homo sapiensP11387 (AlphaFold model)
Sequence of entity 1 (B), FASTA
>1LPQ_1 5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*TP*(8OG)P*GP*AP*AP*AP*AP*AP*TP*TP*TP*TP*T)-3' (chains B)
AAAAAGACTTGGAAAAATTTTT
Sequence of entity 2 (C), FASTA
>1LPQ_2 5'-D(*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*CP*AP*AP*GP*TP*CP*TP*TP*TP*TP*T)-3' (chains C)
AAAAATTTTTCCAAGTCTTTTT
Sequence of entity 3 (A), FASTA
>1LPQ_3 DNA topoisomerase I (chains A)
KWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFA
KMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSK
EEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPED
IIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDW
QKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADT
VGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPE
DDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNR
ANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTK
KVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNFLDPRITVAWCKKWGVPIE
KIYNKTQREKFAWAIDMADEDYEF

Primary citation

8-Oxoguanine rearranges the active site of human topoisomerase I. Lesher, D.T., Pommier, Y., Stewart, L. et al. Proc Natl Acad Sci U S A (2002) 99:12102-12107. DOI 10.1073/pnas.192282699 · PubMed

Other PDB entries of the same protein (UniProt P11387 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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