1R49: DNA topoisomerase I

Human topoisomerase I (Topo70) double mutant K532R/Y723F. Determined by X-ray diffraction at 3.13 Å resolution. Released 16 Dec 2003.

Method
X-ray diffraction
Resolution
3.13 Å
Organism
Homo sapiens
Chains
3
Atoms
5,453
Mol. weight
83.68 kDa
Released
16 Dec 2003

Explore 1R49 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1R49 contains 28 α-helices and 23 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 28 helices, 23 β-strands

ElementResiduesLengthSheet
α-helix211-2122
β-strand220-22121
β-strand22612
α-helix227-2359
β-strand240-24233
β-strand245-24733
α-helix251-26212
α-helix268-2703
α-helix272-28413
α-helix288-2936
β-strand300-30123
α-helix303-31715
α-helix321-33818
β-strand340-34341
β-strand346-34941
β-strand35014
β-strand35412
α-helix355-3584
β-strand359-36025
β-strand373-37425
α-helix375-3784
β-strand383-38536
α-helix392-3954
β-strand403-40536
β-strand414-41746
α-helix4231
β-strand424-42746
β-strand42914
α-helix434-46128
α-helix464-4663
α-helix470-48415
α-helix491-4944
β-strand49517
β-strand49817
β-strand50818
α-helix509-5113
β-strand512-51549
β-strand524-53079
β-strand536-54279
α-helix545-5539
β-strand56318
α-helix570-58011
α-helix588-60316
α-helix612-62615
α-helix648-65912
α-helix662-67413
α-helix679-71032
α-helix726-73510
α-helix740-7423
α-helix746-7516
α-helix753-7575

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
5'-d(*ap*ap*ap*ap*ap*gp*ap*cp*tp*tp*ap*gp*ap*ap*ap*ap*ap*tp*tp*tp*tp*t)-3'BDNA22
5'-d(p*ap*ap*ap*ap*ap*tp*tp*tp*tp*tp*cp*tp*ap*ap*gp*tp*cp*tp*tp*tp*tp*t)-3'CDNA22
DNA topoisomerase IAprotein592Homo sapiensP11387 (AlphaFold model)
Sequence of entity 1 (B), FASTA
>1R49_1 5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*TP*AP*GP*AP*AP*AP*AP*AP*TP*TP*TP*TP*T)-3' (chains B)
AAAAAGACTTAGAAAAATTTTT
Sequence of entity 2 (C), FASTA
>1R49_2 5'-D(P*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*TP*AP*AP*GP*TP*CP*TP*TP*TP*TP*T)-3' (chains C)
AAAAATTTTTCTAAGTCTTTTT
Sequence of entity 3 (A), FASTA
>1R49_3 DNA topoisomerase I (chains A)
KKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEP
LPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNI
ITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANF
KIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWL
VSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVR
QRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGRD
SIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYN
ASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDA
KKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQ
IALGTSKLNFLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

Primary citation

The role of lysine 532 in the catalytic mechanism of human topoisomerase I. Interthal, H., Quigley, P.M., Hol, W.G. et al. J Biol Chem (2004) 279:2984-2992. DOI 10.1074/jbc.M309959200 · PubMed

Other PDB entries of the same protein (UniProt P11387 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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