1SBN: Subtilisin novo

Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes. Determined by X-ray diffraction at 2.1 Å resolution. Released 31 Jan 1994.

Method: X-ray diffraction
Resolution: 2.1 Å
Organisms: Bacillus subtilis, Hirudo medicinalis
Chains: 2
Atoms: 2,781
Mol. weight: 35.78 kDa
Ligands: CA
Released: 31 Jan 1994

Explore 1SBN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1SBN contains 15 α-helices and 31 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain E: 13 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	1
α-helix	6-11	6
α-helix	13-19	7
β-strand	27-32	6	2
β-strand	44-49	6	2
β-strand	51	1	3
β-strand	54	1	3
α-helix	64-73	10
β-strand	80	1	1
β-strand	89-94	6	2
β-strand	101	1	4
α-helix	104-116	13
β-strand	121-124	4	2
β-strand	126-127	2	4
β-strand	128	1	5
α-helix	133-144	12
β-strand	148-152	5	2
β-strand	159	1	6
β-strand	161	1	6
β-strand	167	1	5
β-strand	175-180	6	2
α-helix	185	1
β-strand	186	1	2
α-helix	187	1
β-strand	196-201	6	2
β-strand	205-209	5	7
β-strand	213-217	5	7
β-strand	218	1	8
α-helix	220-235	16
α-helix	243-252	10
α-helix	254	1
β-strand	255	1	2
α-helix	256	1
α-helix	260-263	4
β-strand	267	1	2
α-helix	270-273	4

Chain I: 2 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	9	1	9
α-helix	11-13	3
β-strand	17	1	9
α-helix	18-28	11
β-strand	33-38	6	9
β-strand	43-44	2	4
β-strand	47	1	8
β-strand	51-57	7	9
β-strand	62-63	2	9
β-strand	68-69	2	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Subtilisin novo bpn'	E	protein	275	Bacillus subtilis	P00782 (AlphaFold model)
Eglin C	I	protein	70	Hirudo medicinalis	P01051 (AlphaFold model)

Sequence of entity 1 (E), FASTA

>1SBN_1 SUBTILISIN NOVO BPN' (chains E)
AQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQD
NNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMD
VINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAV
DSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPN
WTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ

Sequence of entity 2 (I), FASTA

>1SBN_2 EGLIN C (chains I)
TEFGSELKSFPEVVGKTVDQAREYFTLHYPQYNVYFLPEGSPVTRDLRYNRVRVFYNPGT
NVVNHVPHVG

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	2

Primary citation

Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes. Heinz, D.W., Priestle, J.P., Rahuel, J. et al. J Mol Biol (1991) 217:353-371. DOI 10.1016/0022-2836(91)90549-L · PubMed

Other PDB entries of the same protein (UniProt P00782 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1TO2 1.3 Å, crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59K,…
1TM5 1.45 Å, crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59A…
1LW6 1.5 Å, Crystal Structure of the Complex of Subtilisin BPN' with Chymotrypsin Inhibitor 2 at 1.5…
1V5I 1.5 Å, Crystal structure of serine protease inhibitor POIA1 in complex with subtilisin BPN'
1Y34 1.55 Å, Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60A…
8H7O 1.55 Å, Crystal structure of aqualigase
1Y1K 1.56 Å, Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 T58A…
1TM3 1.57 Å, crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59k…
1TM7 1.59 Å, crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59Y…
1SUP 1.6 Å, Subtilisin bpn' at 1.6 Å resolution: analysis of discrete disorder and comparison of…
1Y4A 1.6 Å, Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2…
7AM3 1.61 Å, Crystal structure of Peptiligase mutant - M222P

Browse structure collections

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