21DU: Apomorphine bound ADGRG6-Gs complex

Cryo-EM structure of the apomorphine bound ADGRG6-Gs complex. Determined by electron microscopy at 2.9 Å resolution. Released 11 Feb 2026.

Method: Electron microscopy
Resolution: 2.9 Å
Organisms: Homo sapiens, Lama glama
Chains: 5
Atoms: 8,058
Mol. weight: 160.79 kDa
Ligands: OR9
Released: 11 Feb 2026

Explore 21DU in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

21DU contains 34 α-helices and 46 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-39	7	1
α-helix	45-52	8
α-helix	53-55	3
β-strand	69-76	8	1
β-strand	79-86	8	1
α-helix	96-100	5
β-strand	105-111	7	1
α-helix	118-129	12
β-strand	138-144	7	1
α-helix	146-155	10
α-helix	160-163	4
α-helix	166-169	4
α-helix	184-203	20
β-strand	211-215	5	1
α-helix	223-242	20

Chain B: 2 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	38-39	2
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100	1	4
β-strand	104-105	2	5
β-strand	111-113	3	5
β-strand	116	1	4
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	148-151	4	6
β-strand	156-160	5	6
β-strand	165-170	6	6
β-strand	176-181	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	217-222	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	276-278	3	9
β-strand	284-287	4	9
β-strand	293-298	6	9
β-strand	303-309	7	9
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain C: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-24	16
α-helix	26-27	2
α-helix	30-43	14
α-helix	45-47	3

Chain N: 1 helix, 10 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	10
β-strand	17-25	9	10
β-strand	34-39	6	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	68-73	6	10
β-strand	78-84	7	10
α-helix	88-90	3
β-strand	92-98	7	11
β-strand	118	1	11
β-strand	122-124	3	11

Chain R: 17 helices, 0 β-strands

Element	Residues	Length
α-helix	845-847	3
α-helix	859-888	30
α-helix	896-922	27
α-helix	927-955	29
α-helix	956-960	5
α-helix	970-992	23
α-helix	995-998	4
α-helix	1018-1020	3
α-helix	1021-1026	6
α-helix	1027-1052	26
α-helix	1059-1076	18
α-helix	1080-1082	3
α-helix	1083-1086	4
α-helix	1092-1104	13
α-helix	1106-1111	6
α-helix	1112-1116	5
α-helix	1121-1124	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha	A	protein	246	Homo sapiens	A0A590UJY2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
nb35	N	protein	162	Lama glama
Adhesion G-protein coupled receptor G6	R	protein	605	Homo sapiens	Q86SQ4 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>21DU_1 Guanine nucleotide-binding protein G(s) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCRDIIQRMHL
RQYELL

Sequence of entity 2 (B), FASTA

>21DU_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>21DU_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>21DU_4 nb35 (chains N)
MGMKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNW
VRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYC
ARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA

Sequence of entity 5 (R), FASTA

>21DU_5 Adhesion G-protein coupled receptor G6 (chains R)
SNCLKEANEVANQILNLTADGQNLTSANITNIVEQVKRIVNKEENIDITLGSTLMNIFSN
ILSSSDSDLLESSSEALKTIDELAFKIDLNSTSHVNITTRNLALSVSSLLPGTNAISNFS
IGLPSNNESYFQMDFESGQVDPLASVILPPNLLENLSPEDSVLVRRAQFTFFNKTGLFQD
VGPQRKTLVSYVMACSIGNITIQNLKDPVQIKIKHTRTQEVHHPICAFWDLNKNKSFGGW
NTSGCVAHRDSDASETVCLCNHFTHFGVLMDLPRSASQLDARNTKVLTFISYIGCGISAI
FSAATLLTYVAFEKLRRDYPSKILMNLSTALLFLNLLFLLDGWITSFNVDGLCIAVAVLL
HFFLLATFTWMGLEAIHMYIALVKVFNTYIRRYILKFCIIGWGLPALVVSVVLASRNNNE
VYGKESYGKEKGDEFCWIQDPVIFYVTCAGYFGVMFFLNIAMFIVVMVQICGRNGKRSNR
TLREEVLRNLRSVVSLTFLLGMTWGFAFFAWGPLNIPFMYLFSIFNSLQGLFIFIFHCAM
KENVQKQWRQHLCCGRFRLEFLEVLFQGPWSHPQFEKGGGSGGGSGGSAWSHPQFEKDYK
DDDDK

Ligands and cofactors

ID	Name	Formula	Copies
OR9	(6aR)-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-10,11-diol	C17 H17 N O2	1

Primary citation

Extra-helical allosteric binding site of apomorphine in ADGRG6. Qiu, N., Xu, W., Xu, T. et al. Cell Discov (2026) 12:9-9. DOI 10.1038/s41421-025-00866-1 · PubMed

Other PDB entries of the same protein (UniProt A0A590UJY2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VMG 2.82 Å, Cryo-EM structure of the spermine-bound sea lamprey TAAR348-Gs complex
8K4S 2.9 Å, CryoEM structure of Gq coupled MRGPRX4 with agonist DCA-3P
9LWC 2.9 Å, Cryo-EM structure of epinephrine bound dopamine receptor 1…
9DQH 2.92 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-2825
9IZG 3.04 Å, Cryo-EM structure of LPA1-Gq complex with LPA
9WQL 3.2 Å, GRM5-Gq Complex Structure
9ASB 3.4 Å, Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq)…

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