9ASB: Human calcium-sensing receptor

Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq) protein in nanodiscs. Determined by electron microscopy at 3.4 Å resolution. Released 17 Apr 2024.

Method: Electron microscopy
Resolution: 3.4 Å
Organism: Homo sapiens
Chains: 5
Atoms: 18,185
Mol. weight: 286.04 kDa
Ligands: CA, PO4, TCR, NAG
Released: 17 Apr 2024

Explore 9ASB in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9ASB contains 99 α-helices and 107 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-39	6	25
α-helix	42-44	3
α-helix	46-55	10
β-strand	73-76	4	25
β-strand	79-83	5	25
β-strand	105-111	7	25
α-helix	118-130	13
α-helix	132-134	3
β-strand	139-144	6	25
α-helix	146-154	9
α-helix	160-162	3
α-helix	171-173	3
α-helix	184-202	19
β-strand	211-215	5	25
α-helix	223-242	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	26
β-strand	58-63	6	27
β-strand	69-74	6	27
β-strand	78-83	6	27
β-strand	89-94	6	27
β-strand	102-105	4	28
β-strand	111-115	5	28
β-strand	120-125	6	28
β-strand	135-140	6	28
β-strand	146-151	6	29
β-strand	156-161	6	29
β-strand	165-170	6	29
β-strand	176-181	6	29
β-strand	187-192	6	30
β-strand	198-203	6	30
β-strand	208-212	5	30
β-strand	218-222	5	30
β-strand	229-232	4	31
β-strand	240-245	6	31
β-strand	250-254	5	31
β-strand	259-264	6	31
α-helix	272	1
β-strand	273-278	6	32
β-strand	284-289	6	32
β-strand	294-298	5	32
β-strand	303-307	5	32
β-strand	315-320	6	26
β-strand	327-331	5	26
β-strand	335-339	5	26

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	30-43	14

Chain Q: 40 helices, 37 β-strands

Element	Residues	Length	Sheet
β-strand	26-28	3	1
β-strand	32-38	7	1
β-strand	41-44	4	2
α-helix	57-59	3
β-strand	60-63	4	2
α-helix	65-83	19
β-strand	93-99	7	1
α-helix	104-114	11
α-helix	116-122	7
β-strand	138-142	5	1
α-helix	147-157	11
β-strand	164-166	3	1
α-helix	172-175	4
β-strand	183-185	3	1
β-strand	188	1	3
α-helix	191-203	13
β-strand	208-214	7	4
α-helix	219-232	14
β-strand	236-243	8	4
α-helix	249-261	13
β-strand	266-270	5	4
α-helix	273-286	14
α-helix	291	1
β-strand	292-295	4	4
α-helix	308-310	3
α-helix	311-314	4
β-strand	318-321	4	4
β-strand	325	1	5
α-helix	330-335	6
β-strand	345	1	1
α-helix	348-355	8
β-strand	359	1	6
β-strand	394	1	6
α-helix	401-403	3
β-strand	414	1	5
α-helix	416-435	20
α-helix	445-447	3
α-helix	452-454	3
α-helix	457-465	9
β-strand	468-470	3	7
β-strand	476-478	3	7
β-strand	485	1	3
β-strand	489-496	8	4
β-strand	503-511	9	4
α-helix	516	1
β-strand	521-523	3	4
α-helix	525-527	3
β-strand	530	1	8
β-strand	534	1	8
α-helix	537-539	3
α-helix	544-546	3
β-strand	550-554	5	9
β-strand	563-567	5	9
α-helix	568-569	2
β-strand	572-573	2	10
β-strand	579	1	9
β-strand	583-584	2	10
β-strand	589-591	3	11
β-strand	598-600	3	11
α-helix	601	1
β-strand	602-604	3	12
α-helix	611-636	26
α-helix	641-645	5
α-helix	648-664	17
α-helix	665-667	3
α-helix	674-698	25
α-helix	703-707	5
α-helix	723-745	23
β-strand	749-753	5	12
β-strand	760-766	7	12
α-helix	770-792	23
α-helix	799-826	28
α-helix	832-849	18
α-helix	850-854	5
α-helix	855-861	7
α-helix	864-866	3
α-helix	869-872	4

Chain R: 43 helices, 36 β-strands

Element	Residues	Length	Sheet
β-strand	25-28	4	13
β-strand	32-38	7	13
β-strand	41	1	14
β-strand	44	1	15
α-helix	54-59	6
β-strand	60	1	15
β-strand	63	1	14
α-helix	65-83	19
β-strand	93-99	7	13
α-helix	104-114	11
α-helix	116-123	8
β-strand	138-142	5	13
α-helix	147-159	13
β-strand	164-166	3	13
α-helix	172-175	4
β-strand	183-185	3	13
α-helix	191-203	13
β-strand	208-214	7	16
α-helix	219-232	14
β-strand	236-243	8	16
α-helix	249-260	12
β-strand	266-270	5	16
α-helix	273-285	13
β-strand	292-295	4	16
α-helix	297-300	4
α-helix	308-310	3
α-helix	311-314	4
β-strand	318-322	5	16
α-helix	323-324	2
β-strand	325	1	17
α-helix	330-335	6
β-strand	345	1	13
α-helix	348-356	9
β-strand	359	1	18
β-strand	394	1	18
α-helix	401-403	3
β-strand	414	1	17
α-helix	416-435	20
α-helix	445-447	3
α-helix	452-454	3
α-helix	457-465	9
β-strand	468-470	3	19
β-strand	476-478	3	19
β-strand	489-496	8	16
β-strand	503-511	9	16
β-strand	521-523	3	16
α-helix	525-527	3
β-strand	530	1	20
β-strand	534	1	20
α-helix	537-539	3
α-helix	544-547	4
β-strand	550-554	5	21
α-helix	555	1
β-strand	563-567	5	21
α-helix	568-569	2
β-strand	572-573	2	22
α-helix	582	1
β-strand	583-584	2	22
α-helix	585	1
β-strand	589-591	3	23
β-strand	598-600	3	23
α-helix	601	1
β-strand	602-604	3	24
α-helix	611-636	26
α-helix	641-645	5
α-helix	648-664	17
α-helix	665-668	4
α-helix	674-706	33
α-helix	719-745	27
β-strand	749-753	5	24
β-strand	760-766	7	24
α-helix	770-793	24
α-helix	799-801	3
α-helix	804-820	17
α-helix	822-825	4
α-helix	831-849	19
α-helix	850-854	5
α-helix	855-861	7
α-helix	864-866	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 1 of Extracellular calcium-sensing receptor	Q, R	protein	911	Homo sapiens	P41180 (AlphaFold model)
Chimeric mini guanine nucleotide-binding protein G(i)(s)(q) subunit alpha	A	protein	246	Homo sapiens	A0A590UJY2 (AlphaFold model), P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	348	Homo sapiens	P62873 (AlphaFold model)
Chimeric mini guanine nucleotide-binding protein G(i)(s)(q) subunit alpha	G	protein	71	Homo sapiens	P59768

Sequence of entity 1 (Q, R), FASTA

>9ASB_1 Isoform 1 of Extracellular calcium-sensing receptor (chains Q, R)
MAFYSCCWVLLALTWHTSADYKDDDDKYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLK
SRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATL
SFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSR
LLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEE
RDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKI
WLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFW
EETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFRPLCTGDENISSVETPYIDYT
HLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNN
MGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSG
FSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSN
ENHTSCIAKEIEFLSWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSY
LLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPT
SFHRKWWGLNLQFLLVFLCTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCHEGSLMA
LGFLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKF
VSAVEVIAILAASFGLLACIFFNKIYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRS
NVSRKRSSSLG

Sequence of entity 2 (A), FASTA

>9ASB_2 Chimeric mini guanine nucleotide-binding protein G(i)(s)(q) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 3 (B), FASTA

>9ASB_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MDYKDDDDKSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLR
GHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYV
ACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIE
TGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAI
CFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDF
NCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9ASB_4 Chimeric mini guanine nucleotide-binding protein G(i)(s)(q) subunit alpha (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	6
PO4	Phosphate ion	O4 P	2
TCR	Cyclomethyltryptophan	C12 H12 N2 O2	2
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	6
A1AF7	(19R,22S,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosp…	C40 H77 O10 P	1
Y01	Cholesterol hemisuccinate	C31 H50 O4	1
9IG	3-(2-chlorophenyl)-N-[(1R)-1-(3-methoxyphenyl)ethyl]propan-1-amine	C18 H22 Cl N O	2

Primary citation

Promiscuous G-protein activation by the calcium-sensing receptor. Zuo, H., Park, J., Frangaj, A. et al. Nature (2024) 629:481-488. DOI 10.1038/s41586-024-07331-1 · PubMed

Other PDB entries of the same protein (UniProt P41180 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5FBK 2.1 Å, Crystal structure of the extracellular domain of human calcium sensing receptor
7M3G 2.5 Å, Asymmetric Activation of the Calcium Sensing Receptor Homodimer
5K5S 2.6 Å, Crystal structure of the active form of human calcium-sensing receptor extracellular…
5FBH 2.7 Å, Crystal structure of the extracellular domain of human calcium sensing receptor with…
7SIL 2.7 Å, Structure of positive allosteric modulator-bound active human calcium-sensing receptor
7SIM 2.7 Å, Structure of positive allosteric modulator-free active human calcium-sensing receptor
8WPG 2.7 Å, Human calcium-sensing receptor bound with cinacalcet in detergent
7M3F 2.8 Å, Asymmetric Activation of the Calcium Sensing Receptor Homodimer
8SZF 2.8 Å, Cryo-EM structure of cinacalcet-bound active-state human calcium-sensing receptor CaSR…
9C1P 2.8 Å, Structure of Calcium-Sensing Receptor in complex with positive allosteric modulator '6218
9C2F 2.8 Å, Structure of Calcium-Sensing Receptor in complex with positive allosteric modulator '54149
7E6T 3.0 Å, Structural insights into the activation of human calcium-sensing receptor

Browse structure collections

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