2A31: Trypsin

Trypsin in complex with borate. Determined by X-ray diffraction at 1.25 Å resolution. Released 4 Jul 2006.

Method: X-ray diffraction
Resolution: 1.25 Å
Organism: Sus scrofa
Chains: 1
Atoms: 2,199
Mol. weight: 24 kDa
Ligands: CA, MG, BO4, PG3
Released: 4 Jul 2006

Explore 2A31 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2A31 contains 9 α-helices and 20 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	1
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-34	5	3
β-strand	40-48	9	3
β-strand	51-54	4	3
α-helix	56-58	3
β-strand	64-67	4	3
β-strand	72	1	4
β-strand	81-90	10	3
β-strand	104-108	5	3
α-helix	111-114	4
β-strand	122	1	2
α-helix	123-124	2
α-helix	128-130	3
β-strand	135-140	6	2
β-strand	154	1	4
β-strand	156-161	6	2
β-strand	162	1	5
α-helix	163-164	2
α-helix	165-171	7
β-strand	180-182	3	2
β-strand	183	1	5
β-strand	189	1	1
β-strand	198-201	4	2
β-strand	204-215	8	2
β-strand	227-230	4	2
α-helix	231-233	3
α-helix	235-244	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Trypsin	A	protein	223	Sus scrofa	P00761 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2A31_1 Trypsin (chains A)
IVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEG
NEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISG
WGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGFLEGGKDSCQGDSGGP
VVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIAAN

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1
MG	Magnesium ion	Mg	3
BO4	Borate ion	B H4 O4	2
PG3	Guanidine-3-propanol	C4 H12 N3 O	1

Water and common crystallization additives (NA, SO4) are not listed.

Primary citation

NMR and crystallographic characterization of adventitious borate binding by trypsin. Transue, T.R., Gabel, S.A., London, R.E. Bioconjug Chem (2006) 17:300-308. DOI 10.1021/bc0502210 · PubMed

Other PDB entries of the same protein (UniProt P00761 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9PDA 1.18 Å, Structure of Porcine Trypsin Crystals Grown From PEG and Complexed With Crystallization…
1S83 1.25 Å, Porcine trypsin complexed with 4-amino propanol
9PDC 1.27 Å, Porcine Trypsin grown from PEG and Complexed with Crystallization Additives II
9PD9 1.28 Å, The Structure of Porcine Trypsin in Complex with Crystallization Additives I
1S5S 1.4 Å, Porcine trypsin complexed with guanidine-3-propanol inhibitor
9PCT 1.41 Å, Structure of Porcine Trypsin Crystals Grown from PEG Complexed with Crystallization…
1S6H 1.45 Å, Porcine trypsin complexed with guanidine-3-propanol inhibitor
1H9H 1.5 Å, Complex of eeti-II with porcine trypsin
2A32 1.5 Å, Trypsin in complex with benzene boronic acid
1FNI 1.6 Å, Crystal structure of porcine beta trypsin with 0.01% polydocanol
1MCT 1.6 Å, The refined 1.6 Å resolution crystal structure of the complex formed between porcine…
1QQU 1.63 Å, Crystal structure of porcine beta trypsin with bound acetate ion

Browse structure collections

About this viewer

MolViewer shows 2A31 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.