2CAZ: ESCRT-I core

ESCRT-I core. Determined by X-ray diffraction at 3.6 Å resolution. Released 7 Apr 2006.

Method: X-ray diffraction
Resolution: 3.6 Å
Organism: SACCHAROMYCES CEREVISIAE
Chains: 6
Atoms: 3,602
Mol. weight: 74.66 kDa
Released: 7 Apr 2006

Explore 2CAZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2CAZ contains 18 α-helices and 0 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and D: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	326-350	25
α-helix	358-381	24

Chain B: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	31-57	27
α-helix	64-82	19
α-helix	88-93	6
α-helix	97-103	7
α-helix	111-116	6

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	141-170	30
α-helix	178-201	24

Chain E: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	33-57	25
α-helix	64-82	19
α-helix	88-93	6
α-helix	97-103	7
α-helix	111-114	4

Chain F: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	151-170	20
α-helix	178-200	23

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease	A, D	protein	82	SACCHAROMYCES CEREVISIAE	P25604 (AlphaFold model)
Vacuolar protein sorting-associated protein VPS28	B, E	protein	155	SACCHAROMYCES CEREVISIAE	Q02767 (AlphaFold model)
Protein SRN2	C, F	protein	85	SACCHAROMYCES CEREVISIAE	Q99176 (AlphaFold model)

Sequence of entity 1 (A, D), FASTA

>2CAZ_1 SUPPRESSOR PROTEIN STP22 OF TEMPERATURE-SENSITIVE ALPHA-FACTOR RECEPTOR AND ARGININE PERMEASE (chains A, D)
MSTSHVDDEDVNSIAVAKTDGLNQLYNLVAQDYALTDTIECLSRMLHRGTIPLDTFVKQG
RELARQQFLVRWHIQRITSPLS

Sequence of entity 2 (B, E), FASTA

>2CAZ_2 VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS28 (chains B, E)
MAHHHHHHMQKHNIKLNQNQDISQLFHDEVPLFDNSITSKDKEVIETLSEIYSIVITLDH
VEKAYLKDSIDDTQYTNTVDKLLKQFKVYLNSQNKEEINKHFQSIEAFCDTYNITASNAI
TRLERGIPITAEHAISTTTSAPSGDNKQSSSSDKK

Sequence of entity 3 (C, F), FASTA

>2CAZ_3 PROTEIN SRN2 (chains C, F)
MYVASWQDYHSDFSEKYGDIALKKKLEQNTKKLDEESSQLETTTRSIDSADDLDQFIKNY
LDIRTQYHLRREKLATWDKQGNLKY

Primary citation

Escrt-I Core and Escrt-II Glue Domain Structures Reveal Role for Glue in Linking to Escrt-I and Membranes. Teo, H., Gill, D.J., Sun, J. et al. Cell (2006) 125:99. DOI 10.1016/J.CELL.2006.01.047 · PubMed

Other PDB entries of the same protein (UniProt P25604 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3R3Q 1.45 Å, Crystal structure of the yeast Vps23 UEV domain
1UZX 1.85 Å, A complex of the Vps23 UEV with ubiquitin
3R42 1.87 Å, Crystal structure of the yeast vps23 UEV domain in complex with a vps27 PSDP peptide
2F6M 2.1 Å, Structure of a Vps23-C:Vps28-N subcomplex
2P22 2.7 Å, Structure of the Yeast ESCRT-I Heterotetramer Core
2F66 2.8 Å, Structure of the ESCRT-I endosomal trafficking complex

Browse structure collections

About this viewer

MolViewer shows 2CAZ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.